*Corresponding Author: K Chandrasekharan Nair, Professor Emeritus, Department of Prosthodontics, Sri Sankara Dental College, Akathumuri, Thiruvananthapuram, Kerala, India.

Received: May 28, 2024; Published: June 28, 2024

Abstract

Objectives: To compare and evaluate the antimicrobial effect of White vinegar, Copper water and chlorhexidine when used as immersion disinfectants for PMMA resin.

Materials and Methods: One hundred and sixty acrylic denture base resin specimens of dimensions 10 x 10 x 2 mm were made. Four groups of cleansing treatments were carried out. Group 1 - Sterilized distilled water, Group 2 - White vinegar diluted with water (1:1), Group 3 - Chlorhexidine mouth wash (2%), Group 4 – Copper water (sterile water stored in copper vessel for 24 hours). Candida albicans and Staphylococcus aureus were used as reference organisms and used for inoculating the specimens. Viability of organisms were assessed after immersing in the disinfectant media. Colony forming units were counted at the end of I hour and 6 hours. The results were statistically analyzed using one way ANOVA and Tukey’s Post hoc multiple comparisons.

Results: CFU/mL values of S. aureus obtained at the end of one hour were as follows: Gr 1- 1.20 x 104, Gr 2- 0.21 x 104, Gr 3-0.57 x 104 and Gr 4-1.05x104. At the end of six hours the values were 2.46 x 105, 0.20 x 104, 0.37 x 104 and 0.19 x 104 respectively. CFU/mL values of C. albicans obtained at the end of one hour were as follows: Gr. 1-8.43 x 104, Gr 2-0.62x104, Gr 3-1.39 x 104and Gr 4-0.08 x 104. At the end of six hours the values were 4.60 x 105, 0.13 x 104, 0.08 x 104and 0.27 x 104 respectively.

Conclusions

• Copper water which is made by storing water in copper vessel for at least six hours can be used as an immersion disinfectant medium for acrylic prostheses.

• White vinegar is a potent disinfectant medium which can be used as an overnight storage medium for dentures.

• The present study endorses the already established fact that Chlorhexidine gluconate has antimicrobial effect which can be used as an overnight storage medium for dentures.

• All the three media mentioned above have antimicrobial effect against Candida albicans and Staphylococcus aureus which were used as reference organisms.

Keywords: Denture Cleanser; Vinegar; Copper Water; Candida Albicans; Staphylococcus Aureus; Disinfectant Immersion Medium

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Citation

Citation: K Chandrasekharan Nair.,et al. “Comparative Evaluation of Denture Disinfectant Potential of White Vinegar, Copper Water and Chlorhexidine – an Invitro Study".Acta Scientific Dental Sciences 8.7 (2024): 154-158.

Copyright

Copyright: K Chandrasekharan Nair., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.