Materials and Methods: Twenty samples of Heat cure PMMA resin samples (n = 10) and CAD/CAM PMMA resin samples (n = 10) of dimension 12 x 10 x 2.5 mm were prepared. One side of the test sample was considered as uncoated (side A) and opposite side was coated with NANOCOAT surface sealant and considered as e typecoated (side B). The heat cure PMMA resin samples were designated as uncoated (Group IA) and coated (Group IB); the CAD/CAM resin samples were designated as uncoated (Group IIA) and coated (Group IIB). Surface roughness of the test samples were evaluated using surface profilometer and microbial adhesion analysis was done using the spread plate pure culture technique. The results were tabulated and statistically analyzed using Wilcoxon signed ranks test, Mann Whitney U test, Paired t-test and Independent t- test using the SPSS version 2.0 software

Results: Mean surface roughness of Group IA and Group IB was 39.783 nm and 22.960 nm. Mean surface roughness of Group IIA and Group IIB was 27.2130 nm and 18.617 nm. Mean microbial adhesion of Group IA and Group IB was 371 CFU/ml and 248.10 CFU/ml. Mean microbial adhesion of Group IIA and Group IIB was 130.70 CFU/ml and 80.800 CFU/ml. Reduction in surface roughness was noticed after coating with the surface sealant in both Heat cure PMMA and CAD /CAM PMMA resin groups, decrease is more in CAD/CAM PMMA resin samples but was statistically insignificant (P > 0.05). Highly significant reduction in microbial adhesion was noticed after coating with surface sealant in both the groups with CAD/ CAM PMMA resin group showing a higher significant reduction (P < 0.001).

Conclusion: CAD/CAM PMMA resin with sealant application had significantly reduced the microbial adhesion of Streptococcus mutans.

Keywords: Polymethylmethacrylate; Sealant; Adhesion; Resin; Colony Forming Units

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Citation

Citation: Divya Krishnan., et al. “Comparative Evaluation of The Effect of Surface Sealant on the Surface Topography and Microbial Adhesion of Long-Term Provisional Fixed Implant Prosthesis- Invitro Study".Acta Scientific Dental Sciences 7.2 (2023): 164-177.

Copyright

Copyright: © 2023 Divya Krishnan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.