Comparative Evaluation of The Effect of Surface Sealant on the Surface Topography and
Microbial Adhesion of Long-Term Provisional Fixed Implant Prosthesis- Invitro Study
Sivasankari S1, Divya Krishnan2*, Jayakrishnakumar S3, Vidhya J4,
Hariharan Ramakrishnan5, Vallabh Mahadevan6 and Azhagarasan NS7
1Prosthodontist, Ragas Dental College, Chennai, India
2Reader, Department of Prosthodontics and Implantology, Ragas Dental College and Hospital, Chennai, India
3Professor and HOD, Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India
4Ragas Dental College and Hospital, Chennai, India
5Professor, Department of Prosthodontics, Ragas Dental College, Chennai, India
6Professor and HOD, Review, Ragas Dental College and Hospital, Chennai, India
7Professor Emeritus, Ragas Dental College, Chennai, India
*Corresponding Author: Divya Krishnan, Department of Prosthodontics and
Implantology, Ragas Dental College and Hospital, Chennai, India.
Received: January 03, 2023; Published: January 20, 2023
Purpose: To evaluate the effect of surface sealant on the surface topography and microbial adhesion to two different long term provisional materials for fixed implant prosthesis.
Materials and Methods: Twenty samples of Heat cure PMMA resin samples (n = 10) and CAD/CAM PMMA resin samples (n = 10) of dimension 12 x 10 x 2.5 mm were prepared. One side of the test sample was considered as uncoated (side A) and opposite side was coated with NANOCOAT surface sealant and considered as e typecoated (side B). The heat cure PMMA resin samples were designated as uncoated (Group IA) and coated (Group IB); the CAD/CAM resin samples were designated as uncoated (Group IIA) and coated (Group IIB). Surface roughness of the test samples were evaluated using surface profilometer and microbial adhesion analysis was done using the spread plate pure culture technique. The results were tabulated and statistically analyzed using Wilcoxon signed ranks test, Mann Whitney U test, Paired t-test and Independent t- test using the SPSS version 2.0 software
Results: Mean surface roughness of Group IA and Group IB was 39.783 nm and 22.960 nm. Mean surface roughness of Group IIA and Group IIB was 27.2130 nm and 18.617 nm. Mean microbial adhesion of Group IA and Group IB was 371 CFU/ml and 248.10 CFU/ml. Mean microbial adhesion of Group IIA and Group IIB was 130.70 CFU/ml and 80.800 CFU/ml. Reduction in surface roughness was noticed after coating with the surface sealant in both Heat cure PMMA and CAD /CAM PMMA resin groups, decrease is more in CAD/CAM PMMA resin samples but was statistically insignificant (P > 0.05). Highly significant reduction in microbial adhesion was noticed after coating with surface sealant in both the groups with CAD/ CAM PMMA resin group showing a higher significant reduction (P < 0.001).
Conclusion: CAD/CAM PMMA resin with sealant application had significantly reduced the microbial adhesion of Streptococcus mutans.
Keywords: Polymethylmethacrylate; Sealant; Adhesion; Resin; Colony Forming Units
- Gratton DG and Aquilino SA. “Interim restorations”. Dental Clinics of North America 48 (2004): 487-497.
- Augusto MG ., et al. “Nanofilled bis-acryl composite resin materials: Is it necessary to polish?”. Journal of Prosthetic Dentistry 124 (2020): 494.
- Al-Dwairi ZN., et al. “A Comparison of the Surface Properties of CAD/CAM and Conventional Polymethylmethacrylate (PMMA)”. Journal of Prosthodontics 4 (2019): 452-457.
- Rayyan MM., et al. “Comparison of interim restorations fabricated by CAD/CAM with those fabricated manually”. Journal of Prosthetic Dentistry 114 (2015): 414-419.
- Alp G., et al. “Comparison of Flexural Strength of Different CAD/CAM PMMA-Based Polymers”. Journal of Prosthodontics 28 (2019): 491- 495.
- Sahin O., et al. “Effect of surface sealant agents on the surface roughness and color stability of denture base materials”. Journal of Prosthetic Dentistry 116 (2016): 610-616.
- Bollen CM., et al. “Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature”. Dental Materials 13 (1997): 258-269.
- Nyvad B and Fejerskov O. “Transmission electron microscopy of early microbial colonization of human enamel and root surfaces in vivo”. Scandinavian Journal of Dental Research 4 (1987): 297-307.
- Brecx M., et al. “An ultrastructural quantitative study of the significance of microbial multiplication during early dental plaque growth”. Journal of Periodontal Research 18 (1983): 177-186.
- Eick S., et al. “Adherence of Streptococcus mutans to various restorative materials in a continuous flow system”. Journal of Oral Rehabilitation 31 (2004): 278-285.
- Buergers R., et al. “Bacterial adhesion of Streptococcus mutans to provisional fixed prosthodontic material”. Journal of Prosthetic Dentistry 98 (2007): 461-469.
- Patras M., et al. “Management of provisional restorations' deficiencies: a literature review”. Journal of Esthetic and Restorative Dentistry 24 (2012): 26-38.
- Burns DR., et al. “Committee on Research in Fixed Prosthodontics of the Academy of Fixed Prosthodontics. A review of selected dental literature on contemporary provisional fixed prosthodontic treatment: report of the Committee on Research in Fixed Prosthodontics of the Academy of Fixed Prosthodontics”. Journal of Prosthetic Dentistry 90 (2003): 474- 497.
- Alp G., et al. “Optical properties and surface roughness of prepolymerized poly (methyl methacrylate) denture base materials”. Journal of Prosthetic Dentistry 121 (2019): 347-352.
- Berger JC., et al. “Surface roughness of denture base acrylic resins after processing and after polishing”. Journal of Prosthodontics 15 (2006): 180-186.
- Taylor R., et al. “Retention of oral microorganisms on cobalt- chromium alloy and dental acrylic resin with different surface finishes”. Journal of Prosthetic Dentistry 80 (1998): 592-597.
- Flury S., et al. “Effect of artificial toothbrushing and water storage on the surface roughness and micromechanical properties of tooth-colored CAD-CAM materials”. Journal of Prosthetic Dentistry 117 (2017): 767-774.
- Serra G., et al. “Surface morphology changes of acrylic resins during finishing and polishing phases”. Dental Press Journal of Orthodontics 18 (2013): 26-30.
- Al-Rifaiy MQ. “The effect of mechanical and chemical polishing techniques on the surface roughness of denture base acrylic resins”. Saudi Dental Journal 22 (2010): 13-17.
- Al-Kheraif AA. “The effect of mechanical and chemical polishing techniques on the surface roughness heat polymerized and visible light cure polymerized acrylic resins”. Saudi Dental Journal 26 (2014): 56-62.
- Pesun IJ., et al. “Effect of finishing and polishing procedures on the gap width between a denture base resin and two long-term, resilient denture liners”. Journal of Prosthetic Dentistry 87 (2002): 311-318.
- Rizzante FAP., et al. “Influence of resin-coating agents on the roughness and color of composite resins”. Journal of Prosthetic Dentistry 122 (2019): 332.
- Dede DO., et al. “Effect of sealant agents on the color stability and surface roughness of nanohybrid composite resins”. Journal of Prosthetic Dentistry 116 (2016): 119-128.
- Azuma A., et al. “Hydrophilic surface modification of acrylic denture base material by silica coating and its influence on Candida albicans adherence”. Journal of Research in Medical and Dental Science 59 (2012): 1-7.
- Albin-Ameer MA., et al. “Effect of Protective Coating on Surface Properties and Candida albicans Adhesion to Denture Base Materials”. Journal of Prosthodontics 29 (2020): 80-86.
- Cunha TR., et al. “Influence of incorporation of fluoroalkyl methacrylate on roughness and flexural strength of a denture base acrylic resins”. Journal of Applied Oral Science 17 (2009): 103-107.
- Kamonwanon P., et al. “SiO2-nanocomposite film coating of CAD/CAM composite resin blocks improves surface hardness and reduces susceptibility to bacterial adhesion”. Dental Materials Journal 36 (2017): 88-94.
- Lenglerdphol S. “The Effects of surface coating agents on surface microhardness of bis-acryl provisional materials”. Mahidol Dental Journal 39 (2019): 165-172.
- Fathi HM., et al. “Nanocryl Coating of PMMA Complete Denture Base Materials to Prevent Scratching”. European Journal of Prosthodontics and Restorative Dentistry 25 (2017): 116-126.
- Santos M., et al. “The effect of parylene coating on the surface roughness of PMMA after brushing”. Journal of Dentistry 41 (2013): 802-808.
- Catelan A., et al. “Influence of surface sealing on color stability and roughness of composite submitted to ultraviolet‐accelerated aging”. Journal of Investigative and Clinical Dentistry 2 (2017): e12203.
- Bagis B., et al. “Effects of protective resin coating on the surface roughness and color stability of resin-based restorative materials”. Scientific World Journal 2014 (2014): 832947.
- Perez Cdos R., et al. “Effect of a glaze/composite sealant on the 3-D surface roughness of esthetic restorative materials”. Operative Dentistry 34 (2009): 674-680.
- Ali AA., et al. “Effectiveness of Coating Acrylic Resin Dentures on Preventing Candida Adhesion”. Journal of Prosthodontics 22 (2013): 445-450.
- Dickinson GL and Leinfelder KF. “Assessing the long-term effect of a surface penetrating sealant”. Journal of the American Dental Association 124 (1993): 68-72.
- Perez Davidi M., et al. “Effect of liquid- polish coating on in vivo biofilm accumulation on provisional restorations: part 1”. Quintessence International 38 (2007): 591-596.
- Dayrell A., et al. “Effect of sealer coating on mechanical and physical properties of permanent soft lining materials”. Gerodontology 29 (2012): 401-407.
- Sesma N., et al. “Effect of denture surface glazing on denture plaque formation”. Brazilian Dental Journal 16 (2005): 129-134.