Chitosan and Xanthan Membrane in Periosteal Injury in the Calvary of Rats
Fernando Biolcati Chiantia*, Antonio Carlos Aloise and Lydia Masako Ferreira
Consultório Odontológico, Chiantia’s Dental Clinic, Rua Dr. Sampaio Peixoto, Campinas, SP, Brazil
*Corresponding Author: Fernando Biolcati Chiantia, Consultório Odontológico, Chiantia’s Dental Clinic, Rua Dr. Sampaio Peixoto, Campinas, SP, Brazil.
Received: April 14, 2021; Published: May 18, 2021
Introduction: The periosteum is a complex structure composed of an external fibrous layer that confers structural integrity and an internal layer, which has osteogenic and repair potential. When an extensive injury occurs, which does not allow spontaneous repair of the periosteum, there is a difficulty in repairing the wound. With this, there is a search for substitute biomaterials, with reparative capacity. Research with bioresorbable polymers, of natural origin such as chitosan, alginate, gelatin, cellulose and their derivatives, combined or used individually, has been applied in tissue engineering in the development of membranes or dermal dressings.
Objective: To evaluate the membrane composed of Chitosan and Xanthan in a periosteal skin lesion in the calvaria of rats.
Method: 24 male Wistar rats, weighing an average of 360g and aged 10 weeks, were used. All rats were subjected to excision of 10 mm2 of skin and periosteum from the underlying calvaria and the covering of the lesion or not. They were distributed in 2 groups: Control group (CG), without chitosan/xanthan membrane, n = 12 and Experimental group (EG), with chitosan/xanthan membrane, n = 12. The rats were euthanized at 2, 4 and 6 weeks after surgery, and specimens of the calvaria were obtained, where macroscopic analysis of the open area was performed. In the macroscopic analysis, using an ImageJ software, the mean values of the areas of the periosteal lesion in the unhealed calvaries were obtained. For the analysis of intra-group variables between 2, 4 and 6 weeks, the Kruskal-Wallis test was used and for inter-group analysis at 2, 4 and 6 weeks, the Wilcoxon test was used. Values of p < 0.05.
Results: In the macroscopic analysis, the amount of raw area, in the 2nd week, the values expressed for the CG and EG were 93.7% and 81.3%, respectively. In the 4th and 6th weeks, the bloody area values of the EG were 52.3% and 11.3% respectively, and in the CG they were 78.8% and 69.9%.
Conclusion: The chitosan/xanthan membrane, in a periosteal skin lesion in the calvaria of rats, accelerated the healing process, being favorable when compared with the control group in the interval of 4 and 6 weeks.
Keywords: Chitosan; Xanthan Membrane; Periosteal Injury; Calvary of Rats
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