Acta Scientific Dental Sciences (ASDS)(ISSN: 2581-4893)

Research Article Volume 5 Issue 2

Substance P (SP) Increases the Adhesion of Oral Squamous Cell Carcinoma (OSCC) To Human Umbilical Vein Endothelial Cells (HUVEC) Through Upregulating Adhesion Molecules; Implication for Metastasis

Moustafa Elhousiny1*, Kate Miller1, Anura Ariyawadana1,2, and Alan Nimmo1*

1College of Medicine and Dentistry, James Cook University, Cairns, Australia
2Griffith Health Institute, Gold Coast Campus, Griffith University, Australia

*Corresponding Author: Moustafa Elhousiny, College of Medicine and Dentistry, James Cook University, Cairns, Australia.

Received: December 02, 2020; Published: January 18, 2021

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Abstract

Objectives: Interaction of cancer cells with endothelial cells plays an important role in metastasis. Our study hypothesised that SP might play a role in the early onset adhesion of OSCC to HUVEC cells lines via upregulating the adhesion molecule expression.

Methods: CAL 27, SCC25, DOK, H157, BICR22 (OSCC) and HUVEC cell lines were used. Adhesion molecules’ expression was measured by flowcytometry. The ECM645 adhesion assay was used for quantification of tumour-endothelial adhesion. Matrix metalloprotienease-2 (MMP-2) expression in OSCC cells was measured by total ELISA kit.

Results: Treatment with SP increased the adhesion of H157, CAL27 and DOK cells in a time-dependent manner, which was inhibited SP receptor antagonist. Monoclonal anti-adhesion antibodies inhibited the adhesion between OSCC and HUVEC. SP treatment increased (very late antigen-4) VLA-4 adhesion molecule in CAL27 and SCC25 cells and (lymphocyte function associated antigen-1) LFA-1 in BICR22. SP treatment increased MMP-2 release but it was not significant.

Conclusion: The study showed that SP could promote early onset oral cancer–endothelial cell adhesion. The study provided insights into the regulatory mechanism of this adhesion that it can occur in acute phase and in pre-cancer cells. These findings may provide potential new therapeutic targets for metastasis prevention.

Keywords: Oral Squamous Cell Carcinoma; Oral Cancer; Substance P; Tumour-endothelial Adhesion; Adhesion Molecules; NK-1R Antagonist

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References

  1. Warnakulasuriya S. “Global epidemiology of oral and oropharyngeal cancer”. Oral Oncology 45.4 (2009): 309-316.
  2. Ord R and BLANCHAERT R. “Current management of oral cancer: A multidisciplinary approach”. The Journal of the American Dental Association 132 (2001): 19-23.
  3. Chiang A and Massagu'e J. “Molecular basis of metastasis”. New England Journal of Medicine 359.26 (2008): 2814-2823.
  4. Lam L., et al. “Retrospective study of survival and treatment pattern in a cohort of patients with oral and oropharyngeal tongue cancers from 1987 to 2004”. Oral Oncology 43.2 (2007): 150-158.
  5. Sargeran K. “Oral Cancer in Tehran, Iran: An approach for understanding disease burden”. Helsingin yliopisto (2008).
  6. Wu Y and Zhou B. “Inflammation: a driving force speeds cancer metastasis”. Cell Cycle (Georgetown, Tex) 8.20 (2009): 3267.
  7. Entschladen F., et al. “Tumor-cell migration, invasion, and metastasis: navigation by neurotransmitters”. The Lancet Oncology 5.4 (2004): 254-258.
  8. Sawada R., et al. “Differential E-selectin-dependent adhesion efficiency in sublines of a human colon cancer exhibiting distinct metastatic potentials”. Journal of Biological Chemistry 269.2 (1994): 1425-1431.
  9. Fukami A., et al. “Macrosphelide B suppressed metastasis through inhibition of adhesion of sLe (x)/E-selectin molecules”. Biochemical and Biophysical Research Communications 291.4 (2002): 1065-1070.
  10. Wang S., et al. “Effect of an anti-CD54 (ICAM-1) monoclonal antibody (UV3) on the growth of human uveal melanoma cells transplanted heterotopically and orthotopically in SCID mice”. International Journal of Cancer 118.4 (2006): 932-941.
  11. Schlesinger M and Bendas G. “Vascular cell adhesion molecule-1 (VCAM-1)-An increasing insight into its role in tumorigenicity and metastasis”. International Journal of Cancer (2014).
  12. Brener S., et al. “A role for the substance P/NK-1 receptor complex in cell proliferation in oral squamous cell carcinoma”. Anticancer Research 29.6 (2009): 2323-2329. 
  13. Gonzalez-Moles MA., et al. “Substance P and NK-1R expression in oral precancerous epithelium”. Oncology Reports 22.6 (2009): 1325-1331.
  14. Martin TA., et al. “Cancer Invasion and Metastasis: Molecular and Cellular Perspective”. In: Madame Curie Bioscience Database Internet. Austin (TX): Landes Bioscience; 2000-2013. 
  15. Orr FW., et al. “Interactions between cancer cells and the endothelium in metastasis”. The Journal of Pathology 190.3 (2000): 310-329.
  16. Okahara H., et al. “Involvement of very late activation antigen 4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) in tumor necrosis factor α enhancement of experimental metastasis”. Cancer Research 54.12 (1994): 3233-3236.
  17. Fuster M and Esko J. “The sweet and sour of cancer: glycans as novel therapeutic targets”. Nature Reviews Cancer 5.7 (2005): 526-542.
  18. Kobayashi H., et al. “Endothelial cell adhesion molecules and cancer progression”. Current Medicinal Chemistry 14.4 (2007): 377-386.
  19. Strilic B and Offermanns S. “Intravascular Survival and Extravasation of Tumor Cells”. Cancer Cell 32.3 (2017): 282-293.
  20. Szebeni GJ., et al. “Inflammation and Cancer: Extra- and Intracellular Determinants of Tumor-Associated Macrophages as Tumor Promoters”. Mediators of Inflammation (2017): 13.
  21. Shah I., et al. “Clinical stage of oral cancer patients at the time of initial diagnosis”. Journal of Ayub Medical College, Abbottabad 22.3 (2010): 61-63.
  22. Pulte D and Brenner H. “Changes in survival in head and neck cancers in the late 20th and early 21st century: a period analysis”. The Oncologist 15.9 (2010): 994-1001.
  23. Van der Waal I. “Are we able to reduce the mortality and morbidity of oral cancer; some considerations”. Medicina Oral, Patologia Oral Y Cirugia Buccal 18.1 (2013): 33.
  24. Muñoz M., et al. “The NK-1 receptor is expressed in human melanoma and is involved in the antitumor action of the NK-1 receptor antagonist aprepitant on melanoma cell lines”. Laboratory Investigation 90.8 (2010): 1259.
  25. Nowicki M., et al. “The predicting role of Substance P in the neoplastic transformation of the hypoplastic bone marrow”. Journal of Clinical Pathology 59.9 (2006): 935-941.
  26. DeFea KA., et al. “The proliferative and antiapoptotic effects of substance P are facilitated by formation of a beta -arrestin-dependent scaffolding complex”. Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000): 11086-11091.
  27. Sun J., et al. “Substance P enhances NF-kappaβ transactivation and chemokine response in murine macrophages via ERK1/2 and p38 MAPK signaling pathways”. American Journal of Physiology Cell Physiology 294.6 (2008): C1586-1596.
  28. Lewis K. “The role of substance P in the progression and complications of secondary brain tumors 2012.
  29. Friberg S, Nystrom A. Cancer Metastases: Early Dissemination and Late Recurrences”. Cancer Growth and Metastasis 8 (2015): CGM.S31244.
  30. Rebhun RB., et al. “Constitutive expression of the alpha4 integrin correlates with tumorigenicity and lymph node metastasis of the B16 murine melanoma”. Neoplasia 12.2 (2012): 173-182.
  31. Liang S and Dong C. “Integrin VLA-4 enhances sialyl-Lewis-x/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions”. American Journal of Physiology-Cell Physiology 295.3 (2008): C701-C707.
  32. Klemke M., et al. “High affinity interaction of integrin α4β1 (VLA‐4) and vascular cell adhesion molecule 1 (VCAM‐1) enhances migration of human melanoma cells across activated endothelial cell layers”. Journal of Cellular Physiology 212.2 (2017): 368-374.
  33. Li H., et al. “HIF-1alpha-activated ANGPTL4 contributes to tumor metastasis via VCAM-1/integrin beta1 signaling in human hepatocellular carcinoma”. Hepatology 54.3 (2011): 910-919.
  34. Deryugina EI and Quigley JP. “Matrix metalloproteinases and tumor metastasis”. Cancer Metastasis Review 25.1 (2006): 9-34.
  35. Li X., et al. “Neurotransmitter substance P mediates pancreatic cancer perineural invasion via NK-1R in cancer cells”. Molecular Cancer Research: MCR 11.3 (2013): 294-302.
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Citation

Citation: Moustafa Elhousiny.,et al. “Substance P (SP) Increases the Adhesion of Oral Squamous Cell Carcinoma (OSCC) To Human Umbilical Vein Endothelial Cells (HUVEC) Through Upregulating Adhesion Molecules; Implication for Metastasis”. Acta Scientific Dental Sciences 5.2 (2021): 08-18.




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