Arayombo Babatunde Elijah* and Arayombo Aramide Femi

Department of Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria

*Corresponding Author: Arayombo Babatunde Elijah, Department of Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria.

Received: July 31, 2023; Published: December 05, 2023

Abstract

This research investigated the effect of ethyl acetate fraction of lycopene (EAFL) on immunohistochemistry of Cystatin C in kidney of streptozotocin-induced diabetic wistar rats.
Diabetes Mellitus (DM) is defined as a metabolic disease characterized by chronic hyperglycemia due to absolute or relative insulin deficiency which complications affect virtually all organ systems. The prevalence of the disease has become increasingly rampant worldwide despite avertable, DM and its complications are leading causes of death globally. Thirty six adult male Wistar rats weighing between 120-160 g were used for this study. The rats were divided into 6 groups (A, B, C, D, E and F.) of 6 rats each. Group A was the normal control that received 20 ml/kg/day of citrate buffer intraperitoneally (i.p) for 30 days . Groups B, C, D and E were induced with 60 mg/kg of streptozotocin in citrate buffer intraperitoneally once daily for three days and observed for one week. Group B animals were left untreated, while Groups C, D, E, and F were treated with ethyl acetate fraction of lycopene (EAFL) at dosages of 20, 40, 60 and 20 mg/kg/day via oral route respectively for 30 days. At the end of the experimental period the rats were sacrificed through cervical dislocation. It was concluded that EAFL preserves the macro-anatomy of the kidney and reversed the chronic hyperglycemic state characterizing DM. The distortion in renal histological morphology was reversed and restoration of the histoarchitectural alteration in the treated diabetic animals were observed. Moreover, the renal cystatin C antigen antibody reactions were only accentuated in the untreated diabetic group while no immune reactivity were observed in high dose EAFL.

Keywords: Ethyl Acetate; Lycopene; Immunohistochemistry; Kidney; Diabetes; Wistar Rats

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Citation

Citation: Arayombo Babatunde Elijah and Arayombo Aramide Femi. “Effect of Ethyl Acetate Fraction of Lycopene on Immunohistochemistry of Cystatin C in Kidney of Diabetic Wistar Rats". Acta Scientific Clinical Case Reports 5.1 (2024): 03-09.

Copyright

Copyright: © 2024 Arayombo Babatunde Elijah and Arayombo Aramide Femi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.