Department Cancer Biology, Pakistan
*Corresponding Author: Waqar Mazhar, Department Cancer Biology, Pakistan.
Received: November 04, 2022; Published: November 28, 2022
Background: Carcinoma of the breast cancer is the most common malignancy in females. Approximately one in every nine Pakistani women is likely to suffer from breast cancer showing an incidence rate of 50/100,000 and breast cancer is growing at an alarming rate in Pakistan. The main causative element remain unknown yet primary risk factors identified are sex, age, parity, genetics, lack of child bearing, breast feeding, higher hormonal levels and individual lifestyle etc. in Indian and Asian women as compared with western women.
Methods: 80 samples were collected from MINAR. DNA isolation from blood samples then amplify BRCA1 and BRCA2 gene and then run on gel electrophoresis.
Results: The 63 (78.75%) out of 80 patients were BRCA2 positive and showed the amplification BRCA2 gene while 17 individuals were negative and not showed the amplification of both BRCA1 and BRCA2 genes, and no BRCA1 amplification (0%) was observed. The most affected female age groups were 31-40 and 41-50 had the highest risk. In this study the average mean of age 50.0 ± 8.1, BRCA2 9.0 ± 2.4 and negative 1.99 ± 0.75 was observed in 80 patients of breast cancer. The maximum patients of BRCA2 were present in 40 age while minimum were observed in 20 and above 60 age. The paired t test was applied to check the correlation between age and BRCA 1and BRCA2 patients. The results indicated that significant (<0.05) correlation was found between age group and appearance of BRCA2 in 80 breast cancer patients. The highest BRCA2 positive patients were observed in Multan (11) district and lowest were found in district Khanewal and Chechawatni (2). The present study concluded that the age is the main factor that cause the mutation in BRCA2 that leads to breast cancer in female belong to southern Punjab.
Keywords: Carcinoma; Breast Cancer; Breast Feeding
Citation: Waqar Mazhar. “Molecular Analysis of Breast Cancer BRCA1 and BRCA2 in Southern Punjab" Acta Scientific Cancer Biology 6.7 (2022): 06-09.
Copyright: © 2022 Waqar Mazhar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.