Acta Scientific Cancer Biology (ASCB) (ISSN: 2582-4473)

Research Article Volume 6 Issue 6

Silymarin Mediates Apoptosis Through Activation of JNK/ERK Signaling Pathway in Human Colon Carcinoma Cells in Response to (60Co) Gamma Radiation

Mitu Lal1*, Saif Ahmad Khan1 and Damodar Gupta2

1Inter University Accelerator Centre (IUAC), Aruna Asafali Marg, New Delhi, India
2Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research and Development Organization (DRDO), Brig. S.K Mazumdar Marg, Timarpur, Delhi, India

*Corresponding Author: Mitu Lal, Inter University Accelerator Centre (IUAC), Aruna Asafali Marg, New Delhi, India.

Received: July 22, 2022; Published: September 18, 2022

Abstract

Colon cancer is the third most common neoplasm worldwide and one of the major causes of death. Thus, major objective of present investigation was to identify and assess a natural molecule available in dietary sources which can sensitize colon carcinoma cells following radiation exposure. HCT-15 and RKO cells were pretreated (-30min) with silymarin followed by radiation (2 and 2.5Gy) exposure and samples were collected, analyzed at different time intervals (0-48 hrs in relation to radiation and silymarin + radiation time point respectively). Expression of proteins related to ERK/ JNK and mode of cell death was measured using western blotting (γ-H2AX, PCNA, ERK/JNK). A significant activation of JNK was observed in both the cells after treatment with silymarin and radiation. In all the samples effect was higher than silymarin alone and radiation with respect to combination group. An increase in apoptosis with increase in DNA damage was found to be accompanied with decreased SOD2 levels (0-48 hr) by combination treated group. In present investigation, silymarin was found to be potent inducer of cell death through ERK/JNK pathway. Silymarin was found to sensitize cells to radiation at non-toxic concentration and sub lethal radiation dose. Silymarin is already being utilized in traditional system of medicine (homeopathy, ayurveda and Chinese system of medicine) and therefore it possesses potential utility in future for treatment of colon cancer.

Keywords: Ionizing Radiation (IR); Silymarin; JNK; ERK; PCNA

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Citation

Citation: Mitu Lal., et al. “Silymarin Mediates Apoptosis Through Activation of JNK/ERK Signaling Pathway in Human Colon Carcinoma Cells in Response to (60Co) Gamma Radiation" Acta Scientific Cancer Biology 6.6 (2022): 01-13.

Copyright

Copyright: © 2022 Mitu Lal., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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