Molecular Detection of HPVs and their Clinical Application in Diagnosis of Cervical Cancer
Jigarkumar Suthar1* and Rajeshkumar Patel2
1Department of Biotechnology, Hemchandracharya North Gujarat University, Patan, Gujarat, India
2Advisor, Genetics Group of Gujarat Diagnostic centre, Mehsana, Gujarat, India
*Corresponding Author: Jigarkumar Suthar, Department of Biotechnology, Hemchandracharya North Gujarat University, Patan, Gujarat, India.
February 15, 2022; Published: March 21, 2022
Context: Cervical cancer is caused by infection of carcinogenic human papilloma virus commonly known as HPV and that is very common in the world. In India, females above the age of 15 years are at the risk for developing cervical cancer. Among world records, 1,22,844 new cases and 67,477 deaths occur only in India. The present study describes the identification of HPV type by PCR and DNA sequencing, the prevalence of specific types of HPV and risk of cervical cancer in Gujarati population.
Materials and Methods: Total 89 samples were collected from the females of different age groups between 21 to 60 years for the diagnosis of the PAP test as per the criteria and transported to the lab. DNA was extracted, quantity and quality were verified. The HPV L1 gene was amplified by using sets of primers; MY09/11 and GP5+/6+. The product amplified by using GP5+/6+ was sequenced.
Results: Out of total 89 samples, two samples were found to be infected by HPV-16 and one each sample by HPV-51, 66 and 90. HPV-16, 51 and 66 are related to high risk of cervical cancer. However, HPV- 90 also carries risk of cervical cancer.
Conclusions: The present study describes the identification of specific types of HPV and risk of cervical cancer in the Indian Gujarati population. Early detection of high risk HPV types may prevent cervical cancer in the population.
Keywords: Cervical Cancer; Human Papillomavirus; PAP Test; Genital Warts; Nested PCR; L1 Gene; Genotype
- Bruni L., et al. “Human Papillomavirus and Related Diseases in India: Summary Report 27 July 2017”. ICO/IARC Information Centre on HPV and Cancer (HPV Information Centre) (2017): 02-71.
- Desai K. “Ninety percent of Ahmedabad women are unaware of cervical cancer: Survey”. DNA India newspaper of September 21, (2010).
- Gargano J., et al. “Human Papillomavirus”. In: Manual for the Surveillance of Vaccine-Preventable Diseases. Centres for disease control and prevention (2017): 1-15.
- Davies P., et al. “A report on the current status of European research on the use of human papillomavirus testing for primary cervical cancer screening”. International Journal of Cancer 118 (2006): 791-796.
- Mehta A., et al. “Study of high risk cases for early detection of cervical cancer by PAPs smear and visual inspection by lugol’s iodine method”. NHL Journal of Medical Science 2 (2013): 65-68.
- Sankaranarayanan R., et al. “HPV Screening for Cervical Cancer in Rural India”. The New England Journal of Medicine 360 (2009): 1385-1394.
- Abreu ALP., et al. “A review of methods for detect human Papillomavirus infection”. Virology Journal 9 (2012): 262.
- Shen-Gunther J and Yu X. “HPV molecular assays: defining analytical and clinical performance characteristics for cervical cytology specimens”. Gynecologic Oncology2 (2011): 263-271.
- Jacobs MV., et al. “A General Primer GP5+/GP6+ Mediated PCR-Enzyme Immunoassay Method for Rapid Detection of 14 High-Risk and 6 Low-Risk Human Papillomavirus Genotypes in Cervical Scrapings”. Journal of Clinical Microbiology3 (1997): 791-795.
- Annunziata C., et al. “Prevalence of unclassified HPV genotypes among women with abnormal cytology”. Infectious Agents and Cancer 13 (2018): 1-8.
- Schmitt M., et al. “Prevalence and viral load of 51 genital human papillomavirus types and three subtypes”. International Journal of Cancer 132 (2013): 2395-2403.
- Quiroga-Garza G., et al. “Unexpected high prevalence of HPV 90 infection in an underserved population”. Archives of Pathology and Laboratory Medicine 137 (2013): 1569-1573.
- Patel KR., et al. “Prevalence of high-risk human papillomavirus type 16 and 18 in oral and cervical cancers in population from Gujarat, West India”. Journal of Oral Pathology and Medicine 43 (2014): 293-297.
- Schiffman M and Kjaer SK. “Chapter 2: Natural history of anogenital human papillomavirus infection and neoplasia”. Journal of National Cancer Institute - Monographs 31 (2003): 14-19.
- Sowjanya AP., et al. “Prevalence and distribution of high-risk human papilloma virus (HPV) types in invasive squamous cell carcinoma of the cervix and in normal women in Andhra Pradesh, India”. BMC Infectious Diseases 5 (2005): 116.
- Sankaranarayanan R., et al. “A cluster randomised controlled trial of visual, cytology and human papillomavirus screening for cancer of the cervix in rural India”. International Journal of Cancer 116 (2005): 617-623.
- Cossellu G., et al. “Prevalence and concordance of oral and genital HPV in women positive for cervical HPV infection and in their sexual stable partners: An Italian screening study”. PLoS one 13 (2018): 1-13.
- Tawe L., et al. “Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR”. Methods X - Elsevier B.V 5 (2018): 569-578.
- Naucler P., et al. “Efficacy of HPV DNA testing with cytology triage and/or repeat HPV DNA testing in primary cervical cancer screening”. Journal of the National Cancer Institute 101 (2009): 88-99.