Acta Scientific Cancer Biology (ASCB) (ISSN: 2582-4473)

Research Article Volume 5 Issue 2

Fine Needle Aspiration Cytology Findings of Breast Lesions in Female Patients Presenting with Palpable Breast Lumps at Makerere University College of Health Sciences, Kampala-Uganda

Kinalwa Sulaiman1, Abila Derrick Bary2 and Mwesigwa Boaz3*

1Department of Pathology, School of biomedical sciences, College of Health Sciences, Makerere University, Kampala, Uganda
2Faculty of Biology, Medicine and Health, University of Manchester, United Kingdom
3Cytology Society of Uganda, Kampala, Uganda

*Corresponding Author: Mwesigwa Boaz, Cytology Society of Uganda, Kampala, Uganda.

Received: January 29, 2021; Published: February 27, 2020

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Abstract

Background: Palpable breast lumps are a common manifestation encountered by physicians during clinical practice. The use of fine-needle aspiration cytology (FNAC) method has proven to be quick, simple and cost-effective in the evaluation of these lumps for benign, atypical and malignant changes. This study aims to determine the type of breast lesions diagnosed by FNAC and to determine the age-wise distribution for such lesions.

Methodology: A prospective study consisting of 291 females presenting with palpable breast lesions was carried out at Makerere College of Health Sciences (MakCHS) pathology laboratory from January 2019 to May 2019. FNAC results were grouped into tier five reporting categories as C1, C2, C3, C4 and C5 in accordance to the International Academy of Cytology (IAC).

Results: Out of the 291 cases examined, 14 (4.8%) were insufficient (C1), 192 (66%) were benign (C2), 9 (3.0%) were atypical (C3), 15 (5.2%) were suspicious of malignancy (C4) and 61 (21%) were malignant (C5). In the benign category, lesions of fibroadenoma were the most commonly diagnosed constituting 110/192 (57.3%) whereas under the malignant category lesions of invasive ductal carcinoma were the most commonly diagnosed constituting 27/61 (44.3%). The peak age group for benign lesions was 21 - 30 years whereas the peak age group for malignant lesions was 41 - 50 years.

Conclusion: Fine-needle aspiration cytology (FNAC) was found to be an effective diagnostic tool in the categorization of palpable breast lumps into benign, malignant, atypical, suspicious and inadequate categories.

Keywords: Breast Cancer; Fine Needle Aspiration Cytology; Palpable Breast Masses

Abbreviations

ADH: Atypical Ductal Hyperplasia; AUS: Atypia of Undetermined Significance; CBE: Clinical Breast Examination; FA: Fibroadenoma; FCC: Fibrocystic Changes; FNAC: Fine Needle Aspiration Cytology; IAC: International Academy of Cytology; IDC: Invasive Ductal Carcinoma; MakCHS: Makerere University College of Health Sciences; SPSS: Statistical Package for Social Sciences

Introduction

  Breast lump is the localized swelling, protuberance or mass in the breast and is one of the prime indicators of breast disorders. A lump might be a cyst, a benign tumour or a malignancy [1]. Symptom misinterpretation, misdiagnosis and late presentation at the primary health care level are reported as one of contributors to the increased of progression to breast cancer [2]. Globally, over 2 million females are diagnosed with breast cancer accounting for an excess of 626,000 deaths [3]. In the 2018, the age-standardized incidence rate and mortality rate for breast cancer in Uganda was 21.3 per 100,000 and 10.3 per 100,000 population, respectively [4]. These high mortality rates were attributed to a late stage disease diagnosis of women with breast cancer with up to 89% presenting with stage disease that was too difficult to treat [5]. The recommended approach for the early detection and diagnosis of breast lumps involves the use of triple assessment which includes; clinical breast examination (CBE), imaging (ultrasound or mammography) and fine-needle aspiration cytology [6]. Fine-needle aspiration cytology (FNAC) has been in use since the 1930s and is simple, quick and a cost-effective method of diagnosing palpable breast lumps [7]. Whereas this method offers a reliable means of diagnosis during the assessment of breast diseases, its utility in this country is restricted to a few public or private health institutions and limited data is available regarding the prevalence of breast diseases in Uganda.

Aim of the Study

  The aim of this study was to document the type of breast diseases diagnosed by FNAC at the MakCHS pathology laboratory over a period of 5 months (January - May 2019). Furthermore, this study aimed at determining the age-wise distribution of breast diseases at the pathology laboratory over the said period.

Materials and Methods

Study design

The study was a cross-sectional prospective study.

Study area

  This study was conducted at the cytology clinic of the department pathology of Makerere University College of Health Sciences (MakCHS). It is located at Mulago National Referral and Teaching Hospital in Kampala. It is one of fewest public laboratory that offers cytology services.

Study duration

The study was carried out in five months starting from January 2019 to May 2019.

Study population

The study population consisted of all female patients that presented with palpable breast lumps.

Sample size estimation

  For calculation of sample size, the formula below was used [8]: n = Z2P (1-P)/d2, where Z is the value of 1.96 (z in normal distribution curve), 𝑛 is the required sample size, p is the estimated prevalence, and d is precision error (5%). A prevalence p of 25.5% [9] was used in the estimation of sample size, n. Using, this equation, a total sample size of 291 individuals was computed.

Selection criteria Inclusion criteria
  • All female patients who presented with palpable breast lumps.
  • Patients who were able to give consent.
Exclusion criteria
  • The patients with an already known malignancy were excluded.
  • Patients who were severely sick hence couldn’t undergo FNAC procedure.
Sample collection technique, processing and reporting

  Verbal consent was taken after explanation of the procedure and its benefits to the patient. In the FNAC technique, equipment used involved glass slides, disposable gloves, fixatives (70%, 95% alcohol), swabs, 23 gauge needle and 10 ml syringes. The patient was made to lie supine on an examination couch after exposure of upper half of the body. Gloving of hands was done by the resident pathologist in order to perform a breast examination. This examination involved: an assessment of the size of the breast, overlying skin appearance, quadrants and presence or axillary lymph nodes. Thereafter the skin of the breast over the lump was disinfected with 70% alcohol in a swab.

  The lump was immobilised and stabilised between the index finger and the thumb of the left hand. A 23gauge needle attached to a 10 ml syringe was inserted into the nodule. The plunger was retracted to create a vacuum in the needle for suction. Backward and forward movements were used under constant suction with the needle moved at different depths and angles within the confines of the nodule. The process was terminated when fluid appeared in the hub of the needle. The plunger was released to prevent aspiration of the material into the syringe. The needle was removed from the nodule and syringe detached. The syringe was re-attached after withdrawing the plunger and air was used with the needle tip close to the glass slide, the sample was expressed on the slide. At least two passes were made in two different quadrants of the breast lump. With a sterile swab, pressure was applied over the FNA site for about five minutes.

  The aspirated material was smeared on a slide labelled with the patient’s laboratory number and another labelled slide was placed on the smear to evenly and thinly spread it between the two slides on pulling them apart, this made two smears per pass and therefore four smears per patient. Two slides were air dried whereas the other two were fixed immediately by immersion into 95% alcohol. The air-dried smears were stained with May-Grunwald-Giemsa (MGG) staining technique and the smears fixed in 95% alcohol were stained with Papanicolaou staining technique. The attending cytotechnologist took part in the assessment of specimen adequacy.

  The cytological diagnoses were categorized into five standardized categories in accordance to International academy of cytology reporting criterion for breast cytology; Category I (Insufficient material), Category II (Benign), Category III (Atypical), Category IV (Suspicious, probably in situ or invasive) and Category V (Malignant) [10]. The breast samples from FNAC were further sub-classified into definite breast entities.

Data entry and analysis

The data collected was entered using Epidata software and exported to the Statistical Package for Social Sciences (SPSS) version 17.0 for analysis.

Results

  Out of 291 female patients presenting with palpable breast lesions at the MakCHS pathology laboratory, the category-wise distribution was: 14 (4.8%) insufficient (C1), 192 (66%) benign (C2), 9 (3.0%) atypical (C3), 15 (5.2%) suspicious of malignancy (C4) and 61 (21%) malignant (C5). Thus, the ratio of benign to malignant lesions was 3:1. In the benign category, fibroadenoma was the most commonly diagnosed lesion constituting 110/192 (57.3%) followed by fibrocystic changes constituting of 22/192 (11.5%). In the malignant category, invasive ductal carcinoma was the most commonly diagnosed lesion constituting 27/61 (44.3%) followed by Invasive ductal carcinoma of No Specific type (NST) constituting 14/61 (23.0%). The peak age group for benign lesions was 21 - 30 years whereas the peak for malignant lesions was 41 - 50 years. These findings were summarized in table 1-3 respectively.

IAC Classification

Category

Frequency

Insufficient

C1

14 (4.8%)

Benign

C2

192 (66%)

Atypical

C3

9 (3.0%)

Suspicious of Malignancy

C4

15 (5.2%)

Malignant

C5

61 (21%)

Total

 

291

Table 1
Category-wise distribution of cases in accordance to International Academy of Cytology.

Cytological result

Frequency (%)

Insufficient C1

n = 14

No epithelial cells or histiocytes

14 (100.0)

Benign C2

n = 192

Acute mastitis

7 (3.6)

Chronic mastitis

8 (4.2)

Cystic degeneration

8 (4.2)

Duct ectasia

4 (2.1)

Fibroadenoma (FA)

110 (57.3)

Fibroadenoma with chronic mastitis

1 (0.5)

Fibrocystic changes (FCC)

22 (11.5)

Galactocele

9 (4.7)

Lactating adenoma

1 (0.5)

Lipoma

2 (1.0)

Normal breast aspirate (Negative for Malignancy)

18 (9.4)

Tuberculous mastitis

2 (1.0)

Atypical C3

n = 9

Atypical ductal cells of undetermined significance (AUS)

1 (11.1)

Atypical ductal hyperplasia (ADH)

8 (88.9)

Suspicious of Malignancy C4

n = 15

Low- grade ductal carcinoma in situ (Nuclear grade 1)

1 (6.7)

High-grade ductal carcinoma in situ (Nuclear grade 3)

14 (93.3)

Malignant C5

n = 61

Adenocarcinoma of Breast

10 (16.4)

Invasive ductal carcinoma (IDC)

27 (44.3)

Invasive ductal carcinoma of No Specific type (NST)

14 (23.0)

Invasive ductal papilloma

9 (14.8)

Metaplastic carcinoma

1 (1.6)

Table 2
Type-specific distribution of cases as diagnosed by FNAC.

Age-group

Insufficient (C1)

Benign (C2)

Atypical (C3)

Suspicious (C4)

Malignant (C5)

Total

11 - 20

6

25

-

-

-

31

21 - 30

5

70

-

-

-

75

31 - 40

3

52

1

-

5

61

41 - 50

-

20

2

-

33

55

51 - 60

-

15

2

3

10

30

61 - 70

-

8

1

6

6

21

71 - 80

-

2

3

6

7

18

Total

14

192

9

15

61

291

Table 3
Age-wise distribution of cases according to breast categories.

Discussion

  Breast lumps are a common clinical presentation in women presenting with breast symptoms. Whether benign or malignant lumps cause anxiety and depression to the patient [11]. Nature of breast lump can be established by a triple test which includes clinical breast examination, imaging and fine-needle aspiration cytology [12]. FNAC is extremely recognized as a reliable procedure for the initial examination of palpable breast masses. It is minimally invasive, cost-effective, safe, simple, rapid and sensitive as compared to biopsy thus reduces the number of open breast biopsies [13].

  In our study, 4.8% cases had inadequate aspirates and were placed in C1 category which was in concordance with studies done by Sudrat N., et al. [14] whereas Bajwa and Tariq [15] had a slightly higher rate (13.6%) of C1 cases. An “inadequate” report was issued as due to scanty or a cellular material within the aspirates, Benign lesions under the C2 category were most commonly diagnosed with a percentage of 66% (192 cases) was congruent to the study done by Sunita., et al. [16] and by Ahmed., et al [17]. Fibroadenoma (Figure 1A and 1B) and fibrocystic changes (Figure 2A and 2B) were the most common benign lesion observed in this study similar to that found by Smita and Suyrakant [18]. In this study, atypical lesions in the category C3 comprised of 3% (9 cases) was in agreement to a study done by Sudarat., et al [19]. Smears in this category showed ductal epithelial cells benign in nature with overcrowding and mild nuclear enlargement. In the C3 category atypical ductal cells of undetermined significance (AUS) was the most diagnosed lesions followed by atypical ductal hyperplasia (ADH). This study showed 5.2% (15 cases) in suspicious for malignancy category (C4). Other studies showed variable results in C4 category, namely Rakhshinda., et al. [20] in their study found 10.3% in 464 cases and Yusuf., et al. [21] found 13.5% in 66 cases. Smears in this category showed cells. In the category C5, malignant lesions accounted for 21% (61 cases) which was in concordance to the study done by Yusuf., et al [21]. Invasive ductal carcinoma (Figure 3) was the most commonly reported lesion which was also found by Bukhari., et al [22]. There was a predominance of benign lesions in an age group of 21 - 40 years and increased malignancy in the age group of 41 - 50 years. Similar findings were found by Shirish., et al. [23] and Almobarak., et al [24].

Figure 1A: Fibroadenoma showing papillary-like pattern (Papanicolaou stain, ×50).

Figure 1B: Fibroadenoma showing cohesive groups of ductal epithelial cells. Admixed with myoepithelial cells (MGG, ×200).

Figure 2A: Fibrocystic change showing apocrine metaplasia (MGG, ×400).

Figure 2B: Fibrocystic changes showing apocrine metaplastic cells in a Honey-comb architecture (Papanicolaou stain, ×200).

Figure 3: Invasive ductal carcinoma of breast showing coarse chromatin pattern and intranuclear inclusions (MGG, ×200).

Limitations of the Study

  The occurrence of unsatisfactory (inadequate) smears in 14 cases was a limitation to this study. This was mainly due the collagenous nature of the lesions and some lesions were less than 1cm in size which caused difficulties in sampling. In future studies, this may be improved through performing FNAC under ultrasound guidance.

Conclusion and Recommendations

  This study demonstrated a predominance of benign lesions in young age (reproductive-age) and an increased malignancy in older age (post-menopausal). Fine-needle aspiration cytology (FNAC) was also found to be an effective diagnostic tool in the categorization of palpable breast lumps into benign, malignant, atypical, suspicious and inadequate categories.

Data Availability

Data is available in hard copies and can be accessed on request.

Conflict of Interest

Nil.

Funding

There was no source of funding for this study.

Ethical Approval

The permission to carry out this study and disseminate its findings was obtained from the head of department pathology of Makerere University College of Health Sciences.

Consent

Verbal consent was obtained from the study participants before they were enrolled in the study.

Authors’ Contributions

  Kinalwa Sulaiman designed the study, participated in slide preparation and interpretation collected data, managed and analyzed it. Abila Derrick Bary was responsible for data analysis, manuscript development, and reading of manuscript. Mwesigwa Boaz was responsible for drafting the manuscript. All authors have read and approved the final manuscript.

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Citation

Citation: Mwesigwa Boaz., et al. “Fine Needle Aspiration Cytology Findings of Breast Lesions in Female Patients Presenting with Palpable Breast Lumps at Makerere University College of Health Sciences, Kampala-Uganda". Acta Scientific Medical Sciences 5.3 (2021): 13-19.




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