Shanthi Kumari*, Vasavi Angeri and Dugyala Yogitha Bala

Department of Microbiology, Osmania University, India

*Corresponding Author: Shanthi Kumari, Assistant Professor, Department of Microbiology, Osmania University, Hyderabad, Telangana, India.

Received: April 15, 2024; Published: May 20, 2024

Abstract

The ocean's microbiological landscape forms a complex and integral part of its ecosystem, with burgeoning research shedding light on its significant metabolic capabilities and resilience within marine environments. Notably, pigments procured from sea-based bacteria have gained attention for their repository of naturally occurring compounds with advantageous pharmacological properties. These pigments are distinguished by their biological colouring features and are gaining recognition for their viability in diverse sectors, encompassing cosmetics, food production, textile manufacturing, print technology, and pharmaceutical science. The present research centres on the isolation and molecular characterization of a pigment-synthesizing bacterial isolate, named VSB3, sourced from marine samples extracted from the Bapatla and Yarada coastal regions. The unearthing of pigment-producing microbes from these oceanic areas, in conjunction with assessing their raw pigment extracts for antimicrobial effectiveness, presents an exciting frontier for the breakthroughs in antibiotic creation. From ten assorted marine bacterial candidates, eight showed capabilities of pigment biosynthesis; however, only a duo displayed considerable antimicrobial action. Confirmatory analyses of these isolates involved meticulous biochemical testing methods, while precise identification was done through analysis of the 16S rRNA gene sequence and subsequent phylogenetic evaluations. Eventually, VSB3 was taxonomically pinpointed as Pseudomonas aeruginosa following in-depth molecular scrutiny. Remarkably, the unrefined pigment solution yielded from VSB3 exhibited a strong antibacterial response against strains such as Bacillus subtilis, Bacillus cereus, and Proteus vulgaris. Manifestly, this inquiry accentuates the auspicious role of marine-derived bacterial pigments in fostering bioactive substances for industrial innovation and advancing medical treatment modalities.

Keywords: Pseudomonas aeruginosa; 16S rRNA; Phylogenetic Analysis; Antibacterial Activity; GC-MS Analysis; Bacterial Pigment; Marine Bacteria

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Citation

Citation: Shanthi Kumari., et al. “Isolation and Molecular Characterization of a Bacteria VSB3 from the Marine Environment and its Antibacterial Activities".Acta Scientific Biotechnology 5.3 (2024): 03-12.

Copyright

Copyright: © 2024 Shanthi Kumari., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.