Alaebo Prince Ogochukwu¹*, Ukpabi-Ugochukwu Jacintha Chigozie¹, Obike Chiemeziem Adanma¹, Onyeabo Chimaraoke¹, Okakpu Joseph Chinemelu¹, Eze Doris Nneamaka², Achilefu Hyacinth Chibueze¹ and Okolie Jennifer Chisom³

¹Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Abia State, Nigeria
²Department of Food Science Technology, Michael Okpara University of Agriculture, Abia State, Nigeria
³Department of Biotechnology, College of Natural Sciences, Federal University of Technology, Owerri, Imo State, Nigeria

*Corresponding Author: Alaebo Prince Ogochukwu, Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Abia State, Nigeria.

Received: May 03, 2023; Published: December 02, 2023

Abstract

The aim of this study was to evaluate the Antioxidative enzymes potentials of; superoxide dismutase (SOD), Glutathione peroxidase (GSH), Catalase (CAT), Lipid peroxidation (MDA), and some liver parameters such as Aspartate transaminase (AST), Alanine transaminase (ALT), Albumin, and Bilirubin level in Antioxidative potential of methanol extract from Citrullus lanatus peel in CCl4 induced oxidative stress in Albino rats. As well as in-vitro antioxidant parameter such as Ferric Reducing Antioxidant Power (FRAP), 2,2-diphenyl-2-picylydrazyl (DPPH), Total Phenolic Content (TPC) and Total Flavonoids Content (TFC) which was both compared with Vitamin C. Twenty-five (25) wistar rats which was grouped into five rats each was used for the in-vivo experiment while crude plant extract was used for the in-vitro determination. The level of Aspartate aminotransferase (AST) in methanol extract of Citrullus lanatus on sillymarin + CCl4, Citrullus lanatus peel low dose + CCl4, Citrullus lanatus peel high dose + CCl4, CCl4 only, and as well as control had 173.66 ± 44.79, 170.90 ± 4.11, 185.00 ± 10.00, 496.66 ± 25.16, 190.00 ± 31.22 respectively shows that Aspartate aminotransferase (AST) has a significant (P > 0.05) increase of CCl4 negative control when compared with sillymarin + CCl4, Citrullus lanatus peel low dose + CCl4, Citrullus lanatus peel high dose + CCl4, and as well as positive control. In ALT, 200mg/kg body weight of sillymarin + CCl4, 200 and 400mg/kg of the extract Citrullus lanatus peel has a significant (P > 0.05) increase of ALT when compared with CCl4 negative control. There was a dose dependent in Albumin which shows non-significant (P > 0.05) in group treated with sillymarin + CCl4, 200 and 400mg/kg of Citrullus lanatus peel and as well as control when compared to negative control. The group treated with sillymarin + CCl4 shows significantly (P < 0.05) decrease of Bilirubin when compared with Citrullus lanatus peel low dose + CCl4, Citrullus lanatus peel high dose + CCl4, and as well as normal control. The result gathered shows that the group treated with 400 mg/kg of Citrullus lanatus peel high dose + CCl4 indicates non-significantly (P > 0.05) increase of glutathione when compared with negative and as well as normal control. The result shows that the group treated with Citrullus lanatus peel low dose + CCl4 indicates non-significantly (P > 0.05) increase of Catalase when compared with negative control. It was observed that the methanol extract contains moderate level of flavonoids and considerably high level of phenolic content. In conclusion, the methanol extract of Citrullus lanatus peel was found to possess antioxidant potentials which can mob up free radical generated in the body and hence manage conditions associated with oxidative stress.

Keywords: Antioxidative Potentials; Methanol Extracts; Citrullus lanatus; CCl4; Oxidative stress; Albino Rats

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Citation

Citation: Alaebo Prince Ogochukwu., et al. “Antioxidative Potential of Methanol Extract of Citrullus lanatus Peel in CCl4 Induced Oxidative Stress in Albino Rats".Acta Scientific Biotechnology 4.5 (2023): 02-06.

Copyright

Copyright: © 2023 Alaebo Prince Ogochukwu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.