Acta Scientific Biotechnology

Research Article Volume 3 Issue 6

Antibiogram of Escherichia coli Isolated from Stool Samples of Non-human Primates (Macaca mulatta)

Bikash Kumar Dwivedi*, Anju Maharjan, Alisha Acharya, Anshu Dangol, Anjila Puri and Shyam Prasad Pant

Department of Microbiology/St. Xavier’s College, Maitighar, Kathmandu, Nepal

*Corresponding Author: Bikash Kumar Dwivedi, Department of Microbiology/St. Xavier’s College, Maitighar, Kathmandu, Nepal.

Received: October 18, 2022; Published: November 30, 2022

Abstract

Antibiotic resistance is one of the alarming issues concerning public health worldwide. Although wild animal populations such as non-human primates (NHPs) are not directly exposed to antibiotics, antibiotic resistance has been detected in them. Previous studies have revealed the possibility of bacterial exchange between NHPs and humans living nearby, including strains associated with intestinal pathology. This study aimed to detect antibiotic-resistant E. coli prevalent as a gut flora in NHPs which show genetic and physiological similarities to humans. For this study, fresh stool samples of Macaca mulatta (M. mulatta) were collected from different sites in Kathmandu valley. A total of 25 samples were collected and 21 distinct colonies of E. coli were isolated and identified. After antibiogram of thus obtained colonies, 5 (23.81%) of the isolates were found to be resistant to Cefotaxime, all of them resistant to Ceftazidime, 3 (14.29%) resistant to Cefazolin and Gentamicin, only one resistant to Chloramphenicol, and all the isolates were sensitive to Meropenem and Nalidixic acid which concluded only 3 (14.29%) isolates to be multi-drug resistant (MDR). A total of 15 (71.42%) isolates were also found to be Extended Spectrum β-lactamase (ESBL) producing, and 8 (38.1%) isolates were found to be resistant to Colistin. This study suggested the presence of antibiotic-resistant gut E. coli in NHPs distributed in various areas of Kathmandu valley which can be transferred to the environment and humans causing a public health threat in the future.

Keywords: Antibiotics; Colistin; ESBL; MDR; Non-Human Primates

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Citation

Citation: Bikash Kumar Dwivedi., et al. “Antibiogram of Escherichia coli Isolated from Stool Samples of Non-human Primates (Macaca mulatta)".Acta Scientific Biotechnology 3.6 (2022): 34-38.

Copyright

Copyright: © 2022 Bikash Kumar Dwivedi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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