Bikash Kumar Dwivedi*, Anju Maharjan, Alisha Acharya, Anshu Dangol, Anjila Puri and Shyam Prasad Pant

Department of Microbiology/St. Xavier’s College, Maitighar, Kathmandu, Nepal

*Corresponding Author: Bikash Kumar Dwivedi, Department of Microbiology/St. Xavier’s College, Maitighar, Kathmandu, Nepal.

Received: October 18, 2022; Published: November 30, 2022

Abstract

Antibiotic resistance is one of the alarming issues concerning public health worldwide. Although wild animal populations such as non-human primates (NHPs) are not directly exposed to antibiotics, antibiotic resistance has been detected in them. Previous studies have revealed the possibility of bacterial exchange between NHPs and humans living nearby, including strains associated with intestinal pathology. This study aimed to detect antibiotic-resistant E. coli prevalent as a gut flora in NHPs which show genetic and physiological similarities to humans. For this study, fresh stool samples of Macaca mulatta (M. mulatta) were collected from different sites in Kathmandu valley. A total of 25 samples were collected and 21 distinct colonies of E. coli were isolated and identified. After antibiogram of thus obtained colonies, 5 (23.81%) of the isolates were found to be resistant to Cefotaxime, all of them resistant to Ceftazidime, 3 (14.29%) resistant to Cefazolin and Gentamicin, only one resistant to Chloramphenicol, and all the isolates were sensitive to Meropenem and Nalidixic acid which concluded only 3 (14.29%) isolates to be multi-drug resistant (MDR). A total of 15 (71.42%) isolates were also found to be Extended Spectrum β-lactamase (ESBL) producing, and 8 (38.1%) isolates were found to be resistant to Colistin. This study suggested the presence of antibiotic-resistant gut E. coli in NHPs distributed in various areas of Kathmandu valley which can be transferred to the environment and humans causing a public health threat in the future.

Keywords: Antibiotics; Colistin; ESBL; MDR; Non-Human Primates

References

1. Foster-Nyarko E., et al. “Genomic diversity of Escherichia coli isolates from non-human primates in the Gambia”. Microbial Genomics 9 (2020): 1-15.
2. Prestinaci F., et al. “Antimicrobial resistance: a global multifaceted phenomenon”. Pathogens and Global Health 109.7 (2015): 309-318.
3. Cristobal-Azkarate J., et al. “Resistance to antibiotics of clinical relevance in the fecal microbiota of Mexican wildlife”. PLoS ONE9 (2014): 3-10.
4. Habeeb MA., et al. “Rapid emergence of ESBL producers in coli causing urinary and wound infections in Pakistan”. Pakistan Journal of Medical Sciences 29.2 (2013): 540.
5. Poirel L., et al. “Polymyxins: antibacterial activity, susceptibility testing, and resistance mechanisms encoded by plasmids or chromosomes”. Clinical Microbiology Reviews 30.2 (2017): 557-596.
6. HiMedia Labs, India.
7. Performance Standards for Antimicrobial Susceptibility Testing, 29^th Edition (2022).
8. Doi Yohei., et al. “The role of horizontal gene transfer in the dissemination of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in an endemic setting”. Diagnostic Microbiology and Infectious Disease1 (2012): 34-38.
9. Huddleston Jennifer R. “Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes”. Infection and Drug Resistance 7 (2014): 167-176.
10. Albrechtova K., et al. “Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals”. PloS One 9.12 (2014).
11. Rawat D and Nair D. “Extended-spectrum ß-lactamases in gram negative bacteria”. Journal of Global Infectious Diseases 3 (2010): 263.
12. Martínez-Martínez Luis., et al. “Activities of imipenem and cephalosporins against clonally related strains of Escherichia coli hyperproducing chromosomal β-lactamase and showing altered porin profiles”. Antimicrobial Agents and Chemotherapy9 (2000): 2534-2536.
13. Jacoby GA., et al. “Identification of extended-spectrum, AmpC, and carbapenem-hydrolyzing -lactamases in Escherichia coli and Klebsiella pneumoniae by disk tests”. Journal of Clinical Microbiology 44 (2006): 1971-1976.
14. Oteo Jesús., et al. “Extended-spectrum β-lactamase producing Escherichia coli: changing epidemiology and clinical impact”. Current Opinion in Infectious Diseases4 (2010): 320-326.
15. Weiss D., et al. “Antibiotic-resistant Escherichia coli and class 1 integrons in humans, domestic animals, and wild primates in rural Uganda”. Applied and Environmental Microbiology 21 (2018): 1632-1650.
16. Glover BA. “Characterization and resistance profiles of selected enteric bacteria isolated from non-human primates at a wildlife-human interface” (Doctoral dissertation, University of Pretoria) (2014).
17. Zhou Y., et al. “Colistin Combined with Tigecycline: A Promising Alternative Strategy to Combat Escherichia coli Harboring blaND5 and mcr-1”. Frontiers in Microbiology 10 (2020).
18. Levy Stuart B and Bonnie Marshall. "Antibacterial resistance worldwide: causes, challenges and responses”. Nature Medicine12 (2004): S122-S129.
19. Barlow Miriam. "What antimicrobial resistance has taught us about horizontal gene transfer”. Horizontal Gene Transfer (2009): 397-411.
20. Swift Benjamin MC., et al. “Anthropogenic environmental drivers of antimicrobial resistance in wildlife”. Science of the Total Environment 649 (2019): 12-20.

Citation

Citation: Bikash Kumar Dwivedi., et al. “Antibiogram of Escherichia coli Isolated from Stool Samples of Non-human Primates (Macaca mulatta)".Acta Scientific Biotechnology 3.6 (2022): 34-38.

Copyright

Copyright: © 2022 Bikash Kumar Dwivedi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.