Acta Scientific Biotechnology

Research Article Volume 3 Issue 5

Phylogenic Relationship Between West African Dwarf Goat and Red Sokoto Goat in Anambra, Nigerian

VC Ejogugua and JC Okonkwo*

Department of Animal Science and Technology, Nnamdi Azikiwe University, Awka, Nigeria

*Corresponding Author: JC Okonkwo, Department of Animal Science and Technology, Nnamdi Azikiwe University, Awka, Nigeria.

Received: August 22, 2022; Published: September 05, 2022

Abstract

Phylogenetic Relationship between Nigerian Goat Breeds and Ecotypes Using Growth Hormone (GH) gene was studied using fifty goats. DNA extraction and amplification using PCR-RFLP were carried out at Molecular Genetics Laboratory of the Department of Animal Science, University of Port Harcourt, Port-Harcourt, River State, and DNA sequencing at International Institute for Tropical Agriculture (IITA), Ibadan, Nigeria. Using standard protocols, the phylogenic tree was established. Tracing the line of evolutionary descent of West Africa Dwarf (WAD) goat and Red Sokoto (RS) goat from a common ancestor, the phylogenic tree showed two major divergences indicating that there are high genetic diversity and similarity of Nigerian goat sub populations. Greater genetic bind between was observed between the WAD from Onitsha axis and RS goats from Anambra, Awka and Onitsha areas. The first divergence was between WAD goats from Anambra area and those from Onitsha, and RS goat breed. In the second divergence, WAD goats from Awka axis differed out-rightly from other WAD goat ecotypes and RS breed, indicating that there is an indigenous breed or line at Awka axis which are different from the WAD goats. The study revealed more similarity among RS goats than WAD goat breed. Obviously, the WAD goats are not true breed, rather, conglomeration of all the midgets and bantam found in the tropics.

Keywords: DNA Extraction; Amplification and Sequencing; PCR-RFLP; Unweighted Pair Group

References

  1. Adebambo AO., et al. “Genetic distance between two popular Nigerian goat breeds used for milk production”. Livestock Research for Rural Development (2011).
  2. Nwachukwu E N., et al. “Performance of pure and crossbred progenies of Red Sokoto and West African Dwarf goats in the Rainforest Zone of South Eastern Nigeria”. Nigerian Journal of Animal Production2 (2012): 41-48.
  3. Okonkwo J C., et al. “Identification of X-chromatin and determination of its incidence in Nigerian goat breeds”. Livestock Research for Rural Development 22 (2010).
  4. “Food and Agricultural Organization of the United Nations” (2011).
  5. Rotimi E A., et al. “Body Characteristics of West African Dwarf (WAD) Goats in Bassa Local Government Area of Kogi State”. World Scientific News 69 (2017): 179-189.
  6. “Federal Department of Livestock and Pest Control Services”. FLDPCS Nigerian. Livestock Resources. National Synthesis (2007): 440.
  7. Missohou A., et al. “Genetic diversity and differentiation in nine West African local goat breeds assessed via microsatellite polymorphism”. Small Ruminant Research 99 (2011): 20-24.
  8. Ojo OA., et al. “Genetic Analysis of Nigerian Indigenous Goat Populations Using Microsattelite Markers”. Iranian Journal of Applied Animal Science (2018).
  9. An XP., et al. “Polymorphism and DNA methylation in the promoter modulate KISS-1 gene expression and are associated with litter size in goats”. Animal Reproduction Science4 (2015): 36-41.
  10. Othman OE., et al. “Single nucleotide polymorphism in Egyptian cattle insulin-like growth factor binding protein-3 gene”. Journal of Genetic Engineering and Biotechnology2 (2014): 143-147.
  11. Rasouli S., et al. “Evaluation of polymorphism in IGF-I and IGFBP-3 genes and their relationship with twinning rate and growth traits in Markhoz goats”. Annals of Animal Science (2016).
  12. Silveira LGG., et al. “Growth hormone 1 gene (GH1) polymorphism as possible markers of the production potentialof beef cattle using the Brazilian Canchim breed as a model”. Genetics and Molecular Biology4 (2008): 874-879.
  13. Zhang ZJ., et al. “Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds”. Genetics and Molecular Research2 (2013): 965-971.
  14. Amie Marini AB., et al. “Genetic polymorphism of growth hormone gene in Savanna goat”. Proc 31st MSAP Conference, MSAP, KotaBharu. (2010): 184-185.
  15. Jia JL., et al. “Study of the correlation between GH gene polymorphism and growth traits in sheep”. Genetics and Molecular Research 3 (2014): 7190-7200.
  16. Sharma A., et al. “Novel SNPs in IGF1, GHR and IGFBP-3 genes reveal significant association with growth traits in Indian goat breeds”. Small Ruminant Research1 (2013): 714.
  17. Okumus A and Mercan L. “Genetic variation at karayaka sheep herds based on random amplified polymorphic DNA (RAPD) markers”. Biotech 4 (2007): 543-548.
  18. Missohou A., et al. “Diversity and genetic relationships among seven West African goat breeds”. Asian-Australasian Journal of Animal Sciences 19 (2006): 1245-1251.
  19. Murital I., et al. “Genetic diversity and population structure of Nigerian indigenous goat using DNA microsatellite markers”. Archivos de Zootecnia 64 (2015): 93-98.
  20. National Bureau of Statistics (NBS) (2009).

Citation

Citation: VC Ejogugua and JC Okonkwo. “Phylogenic Relationship Between West African Dwarf Goat and Red Sokoto Goat in Anambra, Nigerian".Acta Scientific Biotechnology 3.5 (2022): 18-20.

Copyright

Copyright: © 2021 VC Ejogugua and JC Okonkwo. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate33%
Acceptance to publication20-30 days

Indexed In



News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US