Phylogenic Relationship Between West African Dwarf Goat and Red Sokoto Goat in Anambra, Nigerian
VC Ejogugua and JC Okonkwo*
Department of Animal Science and Technology, Nnamdi Azikiwe University, Awka, Nigeria
*Corresponding Author: JC Okonkwo, Department of Animal Science and Technology, Nnamdi Azikiwe University, Awka, Nigeria.
August 22, 2022; Published: September 05, 2022
Phylogenetic Relationship between Nigerian Goat Breeds and Ecotypes Using Growth Hormone (GH) gene was studied using fifty goats. DNA extraction and amplification using PCR-RFLP were carried out at Molecular Genetics Laboratory of the Department of Animal Science, University of Port Harcourt, Port-Harcourt, River State, and DNA sequencing at International Institute for Tropical Agriculture (IITA), Ibadan, Nigeria. Using standard protocols, the phylogenic tree was established. Tracing the line of evolutionary descent of West Africa Dwarf (WAD) goat and Red Sokoto (RS) goat from a common ancestor, the phylogenic tree showed two major divergences indicating that there are high genetic diversity and similarity of Nigerian goat sub populations. Greater genetic bind between was observed between the WAD from Onitsha axis and RS goats from Anambra, Awka and Onitsha areas. The first divergence was between WAD goats from Anambra area and those from Onitsha, and RS goat breed. In the second divergence, WAD goats from Awka axis differed out-rightly from other WAD goat ecotypes and RS breed, indicating that there is an indigenous breed or line at Awka axis which are different from the WAD goats. The study revealed more similarity among RS goats than WAD goat breed. Obviously, the WAD goats are not true breed, rather, conglomeration of all the midgets and bantam found in the tropics.
Keywords: DNA Extraction; Amplification and Sequencing; PCR-RFLP; Unweighted Pair Group
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