Suvekshya Shreshta, Juhi Desai, Mariam Tinawi and Kokila Kota*

Ramapo College of New Jersey, Mahwah, NJ, USA

*Corresponding Author: Kokila Kota, Principle Investigator, Ramapo College of New Jersey, Mahwah, NJ, USA.

Received: August 09, 2022; Published: August 25, 2022

Abstract

Biofilms are a hallmark feature of various opportunistic pathogenic bacteria that allow them to communicate with each other. Pseudomonas aeruginosa is an opportunistic bacterium that results in serious infections in health-care settings, associating with over 70% of all clinical infections. Pseudomonas growing in biofilms exhibit adaptive resistance to essentially all antibiotics and there is an urgent need to work on new methods to supplement our current antibiotics. Therefore, the long-term goal of our research aims to develop anti-biofilm agents targeting polymicrobial communities rather than the planktonic bacteria.

An important group of soil bacteria, recognized as a source of commercially important enzymes and antimicrobials, is the largely underestimated Actinomycetes. Considering the potential of discovering Actinomycetes that can produce enzymes and antimicrobials, this study is aimed to isolate Actinomycetes samples collected from sites across Ramapo College campus soils and test the antimicrobials with industrial and medical applications. Several strains were isolated and screened using standard microbiology and biochemical diagnostic tests. One strain was identified as a thermophile and based on the colony morphology, gram staining and diagnostic tests had identified it to be a gram positive bacteria belonging to the genus Actinomycetes. 

The isolated strain of Actinomycetes was further tested and the extracellular crude extract from the supernatant exhibited anti-bacterial and anti-fungal activity assessed by agar disk diffusion. It also exhibited anti-biofilm activity against the Pseudomonas aeruginosa biofilm. Our research addresses if pre-treatment of the bacterial biofilms with the purified supernatant increases their antibiotic susceptibility. A combined treatment of antibiotics with antibiofilm peptides may offer a very potent treatment of both biofilm and planktonic infections resulting in novel adjuvant therapies.

Keywords: Pseudomonas aeruginosa; Actinomycetes; temperature

References

1. Rijal Nisha. “Starch Hydrolysis Test: Principle, Procedure, Results”. Microbe Online (2021).
2. “MR-VP Test: Introduction, Principle, Procedure, Result Interpretation”. universe84a, (2021).
3. Aryal Sagar. “Gelatin Hydrolysis Test - Principle, Procedure, Uses and Interpretation”. Microbiology Info.com, (2019).
4. Aryal Sagar., et al. “Catalase Test- Principle, Uses, Procedure, Result Interpretation with Precautions”. Microbiology Info.com (2022).
5. Anne Marie Helmenstine, Ph.D. “What Gram Staining Is and How to Do It”. ThoughtCo, ThoughtCo (2019).
6. Aryal Sagar., et al. “Endospore Staining- Principle, Reagents, Procedure and Result”. Microbiology Info.com, (2022).
7. “Ammonium Sulfate Precipitation Protocol”. Accelerating the Pace of Discovery.
8. Tendencia Eleonor. “Disk Diffusion Method”. Southeast Asian Fisheries Development Center (2004).
9. O'Toole George A. “Microtiter Dish Biofilm Formation Assay”. Journal of Visualized Experiments : JoVE, MyJove Corporation (2011).

Citation

Citation: Kokila Kota., et al. “Isolation, Identification and Assessment of the Anti-bacterial, Anti-Fungal and Anti-biofilm Activity of a Novel Temperature Resistant Thermophilic Actinomycetes Gram Positive Bacteria Extracted from the Soil".Acta Scientific Biotechnology 3.4 (2022): 13-20.

Copyright

Copyright: © 2022 Kokila Kota., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.