Acta Scientific Biotechnology

Research Article Volume 3 Issue 2

Exploring the Role of Bioactive Polyphenolic Antioxidants in Salinity Tolerance of Two Rice Landraces from Coastal Areas of Bangladesh

Uthpal Krishna Roy1,2, Ananya Dey2 and Soumen Bhattacharjee2*

1Department of Botany, University of Rajshahi, Bangladesh
2Department of Botany, The University of Burdwan, West Bengal, India

*Corresponding Author: Soumen Bhattacharjee, Professor and Coordinator, UGC Centre for Advanced Study, Department of Botany, The University of Burdwan, West Bengal, India.

Received: March 28, 2022; Published: April 29, 2022


The bioactive polyphenolic compounds (BPC), play an important role in the restoration of cellular redox homeostasis but are seldom studied in context of salinity stress tolerance in rice. RP-HPLC based comparative analysis of some important bioactive polyphenolic compounds (gallic acid, protocatechuic acid, para-hydroxy benzoic acid, catechin, chlorogenic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, sinapic acid, salicylic acid, naringin, rutin, ellagic acid, myricetin, quercetin, naringenin, apigenin and kaempferol) from seedling of two rice landraces (Oryza sativa L. landraces Kutepatnai and Charobalam) subjected to post imbibitional salinity stress (PISS) not only exhibited differential landrace specific accumulation but also exhibited strong correlation with salinity tolerance. The landrace Kutepatnai exhibiting the ability to maintain redox homeostasis under PISS (assessed in terms of biomarkers of oxidative stress like the relative reactive oxygen species accumulation, relative total antioxidant competence and relative oxidative membrane damage) showed significantly greater up-regulation of majority of the polyphenolic compounds derived from chalcone synthase, and cinnamic acid dependent pathway as compared to the salt susceptible landrace Charobalam. The positive correlation between bioaccumulation of BPC (protocatechuic acid, ellagic acid, caffeic acid, syringic acid, rutin, catechin, myricetin, quercetin and apigenin) with redox parameters suggests their role in regulation of redox homeostasis necessary for salt tolerance in experimental land races of coastal areas of Bangladesh.

Keywords: Rice Landraces; Salinity Tolerance; Bioactive Polyphenolic Compounds; Antioxidants; Redox Homeostasis


  1. Horie T and Schroeder JI. “Sodium transporter in plants. Diverse genes and physiological functions”. Plant Physiology 136 (2004): 2457-2462.
  2. Roy SJ., et al. “Salt resistant crop plants”. Current Opinion in Biotechnology 26 (2014): 115-124.
  3. Parida AK and Das AB. “Salt tolerance and salinity effects on plants: A review”. Ecotoxicology and Environmental Safety 60 (2005): 324-349.
  4. Munns R and Tester M. “Mechanisms of salinity tolerance”. Annual Review of Plant Biology 59 (2008): 651-658.
  5. Rahnama A., et al. “Stomatal conductance as a screen for osmotic stress tolerance in durum wheat growing in saline soil”. Functional Plant Biology3 (2010): 255-263.
  6. Arzani A and Ashraf M. “Smart engineering of genetic resources for enhanced salinity tolerance in crop plants”. Critical Reviews in Plant Sciences 35 (2016): 146-189.
  7. Benlloch-Gonzalez M., et al. “Strategies underlying salt tolerance in halophytes are present in Cynara cardunculus”. Plant Science 168 (2005): 635-659.
  8. Rout NP and Shaw BP. “Salt tolerance in aquatic macrophytes: possible involvement of the antioxidative enzymes”. Plant Science 160 (2001): 415-423.
  9. Rabara RC., et al. “Conservation of rice genetic resources for food security”. Advances in food technology and Nutritional Sciences SE1 (2015): S51-S56.
  10. Walters SA. “Essential Role of Crop Landraces for World Food Security”. Modern Concept and Developments in Agronomy5 (2018): 91-95.
  11. Mahmoudi H., et al. “The Impact of Genotype and Salinity on Physiological Function, Secondary Metabolite Accumulation, and Antioxidative Responses in Lettuce”. Journal of Agricultural and Food Chemistry 58 (2010): 5122-5130.
  12. Miller G., et al. “Reactive oxygen species homeostasis and signaling during drought and salinity stresses”. Plant, Cell and Environment 33 (2010): 453-467.
  13. Bhattacharjee S. “ROS and oxidative stress: origin and implication”. In: Reactive oxygen species in plant biology. Springer Nature (2019): 1-31.
  14. Anuradha S and Rao SSR. “Effect of Brassinosteroids on Salinity Stress Induced Inhibition of Seed Germination and Seedling Growth of Rice (Oryza sativa L.)”. Plant Growth Regulation 33 (2001): 151-153.
  15. Gill SS and Tuteja N. “Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants”. Plant Physiology and Biochemistry 48 (2010): 909-930.
  16. Kim D., et al. “Antioxidant capacity of phenolic phytochemicals from various cultivars of plums”. Food Chemistry 81 (2003): 321-326.
  17. Posmyk MM., et al. “Antioxidant Enzymes Activity and Phenolic Compounds Content in Red Cabbage Seedlings Exposed to Copper Stress”. Ecotoxicology and Environmental Safety 72 (2009): 596-602.
  18. Bhattacharjee S and Dey N. “Redox metabolic and molecular parameters for screening drought tolerant indigenous aromatic rice cultivars”. Physiology and Molecular Biology of Plants 24 (2018): 7-23.
  19. Banik N and Bhattacharjee S. “Complementation of ROS scavenging secondary metabolites with enzymatic antioxidant defense system augments redox-regulation property under salinity stress in rice”. Physiology and Molecular Biology of Plants 26 (2020): 1623-1633.
  20. Oh MM., et al. “Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce”. Journal of Plant Physiology 166 (2009): 180-191.
  21. Burchard P., et al. “Contribution of hydroxycinnamates and flavonoids to epidermal shielding of UV-A and UV-B radiation in developing rye primary leaves as assessed by ultraviolet-induced chlorophyll fluorescence measurements”. Plant, Cell and Environment 23 (2000): 1373-1380.
  22. Rice-Evans CA., et al. “Structure-Antioxidant Activity Relationships of Flavonoids and Phenolic Acids”. Free Radical Biology and Medicine 20 (1996): 933-956.
  23. Wang Y and Nil N. “Changes in Chlorophyll, Ribulose Biphosphate Carboxylase-Oxygenase, Glycine Betaine Content, Photosynthesis and Transpiration in Amaranthus tricolor Leaves during Salt Stress”. Journal of Horticultural Science and Biotechnology 75 (2000): 623-627.
  24. Tsai PJ., et al. “Anthocyanin and Antioxidant Capacity in Roselle (Hibiscus sabdariffa L.) Extract”. Food Research International 35 (2002): 351-356.
  25. Zhou K., et al. “Comparison of Swiss Red Wheat Grain and Fractions for Their Antioxidant Properties”. Journal of Agricultural and Food Chemistry 52 (2004): 1118-1123.
  26. Liu RH. “Whole Grain Phytochemicals and Health”. Journal of Cereal Science 46 (2007): 207-219.
  27. Dicko MH., et al. “Impact of phenolic compounds and related enzymes in sorghum varieties for resistance and susceptibility to biotic and abiotic stresses”. Journal of Chemical Ecology 31 (2005): 2671-2688.
  28. Simontacchi M., et al. “Oxidative stress affects -tocopherol content in soyabean embryonic axes upon imbibitions”. Plant Physiology 103 (1993): 949-953.
  29. Mensor LL., et al. “Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method”. Phytotherapy Research 15 (2001): 127-130.
  30. Heath RL and Packer L. “Photo-oxidation in isolated chloroplasts: kinetics and stoichiometry of fatty acid oxidation”. Archives of Biochemistry and Biophysics 125 (1968): 189-198.
  31. Rubio-Casal AE., et al. “Influence of salinity on germination and seeds viability of two primary colonizers of Mediterranean salt pans”. Journal of Arid Environment. 53 (2003): 145-154.
  32. Chakrabarty A., et al. “Redox-regulation of germination during imbibitional oxidative and chilling stress in an indica rice cultivar (Oryza sativa L., Cultivar Ratna)”. Physiology and Molecular Biology of Plants (2019).
  33. Aditya M., et al. “RP-HPLC and GC-MS based identification of phenolic acids, flavonoids and hydroxyl containing compounds from one of the Lead accessions of Amaranthus hypochondriacus L. identified on the basis of biomarkers of antioxidant potential”. Basic and Applied Pharmacology1 (2018): 1-8.
  34. Fiorani F., et al. “The alternative oxidase of plant mitochondria is involved in the acclimation of shoot growth at low temperature. A study of Arabidopsis AOX1a transgenic plants”. Plant Physiology 139 (2005): 1795-1805.
  35. Fini A., et al. “Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants“. Plant Signaling and Behavior 6 (2011): 709-711.
  36. Lee SY., et al. “Influence of salicylic acid on rubisco and rubisco activity in tobacco plant grown under sodium chloride in vitro”. Saudi Journal of Biological Sciences 21 (2014): 417-426.
  37. Hossain MS and Dietz KJ. “Tuning of Redox Regulatory Mechanisms, Reactive Oxygen Species and Redox Homeostasis under Salinity Stress”. Frontiers in Plant Science 7 (2016).
  38. Azevedo Neto A D., et al. “Salinity and oxidative Stress in Abiotic stress and Plant Responses”. (eds. Khan, N. A. & Singh, S.) 58-82 (IK International) (2008).
  39. Turan S and Tripathy B C. “Salt genotype impact on antioxidant enzymes and lipid peroxidation in two rice cultivars during de-etiolation”. Protoplasma (2012).
  40. Azevedo Neto A D., et al. “Salinity and oxidative Stress in Abiotic stress and Plant Responses”. (eds. Khan, N. A. and Singh, S.) 58-82 (IK International), (2008).
  41. Bhattacharjee S. Reactive oxygen species in Plant Biology 1-187 (Springer Nature), (2019).
  42. Dey N and Bhattacharjee S. “Oxidative Membrane Lipid Peroxidation and Accumulation of RedoxSensitive Polyphenolic Compounds Serves as Sensitive Redox-Metabolic Biomarkers of Drought Stress of Rice”. Austin Journal of Plant Biology 1 (2019): 1021-1026.
  43. Sarker U and Oba S. “Salinity stress enhances color parameters, bioactive leaf pigments, vitamins, polyphenols, flavonoids and antioxidant activity in selected Amaranthus leafy vegetables”. Journal of the Science of Food and Agriculture 5 (2019): 2275-2284.


Citation: Soumen Bhattacharjee., et al. “Exploring the Role of Bioactive Polyphenolic Antioxidants in Salinity Tolerance of Two Rice Landraces from Coastal Areas of Bangladesh".Acta Scientific Biotechnology 3.2 (2022): 29-40.


Copyright: © 2021 Soumen Bhattacharjee., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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