Vitamin E From Microbes: Extraction, Analysis and Application
Patel Prachi K, Gohel Payal K, Vora Axita P, Mistry Parth R, Rathod Zalak R* and Saraf Meenu S
Department of Microbiology and Biotechnology, University school of sciences, Gujarat, India
*Corresponding Author: Rathod Zalak R, Department of Microbiology and Biotechnology, University school of sciences, Gujarat, India.
Received:
December 30, 2021; Published: March 19, 2022
Abstract
Different types of important vitamins can’t be made or can be generated but not in adequate quantity by the higher species, so they must be received externally through diet. Some of the natural sources that produce vitamins include several plants, animals, vegetable oils, most fishes and certain microorganisms. Due to higher demand, significant use of microorganisms to produce vitamins is under development and research. Vitamin E is an important and significant antioxidant that is fat-soluble and protects the body from free radicals. There are mainly two types of vitamin E: tocopherol and tocotrienol, which are subdivided into a total of eight types. Shikimate and/or MVA (Mevalonate) or MEP (2-C-methyl-D-erythritol-4-phosphate) Pathway synthesise this. Vitamin E is needed to keep skeletal, heart, and smooth muscle structure and function healthy. It also aids in the development of red blood cells and the maintenance of fat-soluble vitamins A and K, iron and other minerals. Vitamin E deficiency can result in anemia, heart disease, serious neurological problems, poor vision, etc. Although various strains of microorganisms like bacteria, yeasts, and algae are known to be isolated for the production of vitamin E. Extraction processes are employed to obtain an extract which is further purified by certain purification techniques. Various quantitative and qualitative analyses of extracts are used to check the quality and quantify the vitamin content of the particular strain. Further characterization by DNA sequencing method is used to identify strains from unknown sources. The sequences are analysed and an online local alignment tool is utilised to find out the organism.
Keywords: Antioxidant; Extraction; HGA-homogentisic Acid; Tocopherol; Tocotrienol
References
- Angulo-Molina A., et al. “Magnetite nanoparticles functionalized with α-tocopheryl succinate (α-TOS) promote selective cervical cancer cell death”. Journal of Nanoparticle Research8 (2014): 1-12.
- Augustin J. “Methods of vitamin assay”. Wiley (1985).
- Bhushan B., et al. “Techno-functional differentiation of two vitamin B 12 producing Lactobacillus plantarum strains: an elucidation for diverse future use”. Applied Microbiology and Biotechnology2 (2017): 697-709.
- Cahoon EB., et al. “Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content”. Nature Biotechnology9 (2003): 1082-1087.
- Collakova E and DellaPenna D. “Isolation and functional analysis of homogentisatephytyl transferase from Synechocystis sp. PCC 6803 and Arabidopsis”. Plant Physiology3 (2001): 1113-1124.
- DellaPenna D. “A decade of progress in understanding vitamin E synthesis in plants”. Journal of Plant Physiology7 (2005): 729-737.
- Diplock AT., et al. “Tocopherol, ubiquinones and ubichromenols in yeasts and mushrooms”. Nature4766 (1961): 749-750.
- Dubey S and Vyas SP. “Emulsomes for lipophilic anticancer drug delivery: Development, optimization and in vitro drug release kinetic study”. International Journal of Applied Pharmaceutics 13 (2021): 114-121.
- Emmerie A and Engel C. “Colorimetric determination of α‐tocopherol (vitamin E)”. Recueil des TravauxChimiques des Pays‐Bas12 (1983): 1350-1355.
- Evans HM and Bishop KS. “On the existence of a hitherto unrecognized dietary factor essential for reproduction”. Science1458 (1992): 650-651.
- Food NB and Board N. “Institute of Medicine. Dietary reference intakes for vitamin C, vitamin E, selenium and carotenoids (2000).
- Forbes M., et al. “A New Antioxidant from Yeast. Isolation and Chemical Studies1”. Journal of the American Chemical Society2 (1958): 385-389.
- Frega N., et al. “Identification and estimation of tocotrienols in the annatto lipid fraction by gas chromatography-mass spectrometry”. Journal of the American Oil Chemists' Society12 (1998): 1723-1727.
- Gamna F and Spriano S. “Vitamin E: A Review of Its Application and Methods of Detection When Combined with Implant Biomaterials”. Materials13 (2021): 3691.
- Green J., et al. “Tocopherols in micro-organisms”. Nature4695 (1959): 1339-1339.
- Hallmann CA., et al. “More than 75 percent decline over 27 years in total flying insect biomass in protected areas”. Plos One10 (2017): e0185809.
- Hardbower DM., et al. “Chronic inflammation and oxidative stress: the smoking gun for Helicobacter pylori-induced gastric cancer?” Gut Microbes6 (2013): 475-481.
- https://www.healthline.com/health/pregnancy/prenatal-vitamins-not-pregnant
- https://www.healthline.com/nutrition/fat-soluble-vitamins
- https://www.medicalnewstoday.com/articles/321800#takeaway
- https://www.Shimadzu.com/an/service-support/technical-support/analysis-basics/basic/w hat_is_hplc.html#4
- https://www.webmd.com/diet/supplement-guide-vitamin-e
- https://www.webmd.com/diet/supplement-guide-vitamins
- Hughes PE and Tove SB. “Occurrence of alpha-tocopherolquinone and alpha-tocopherolquinol in microorganisms”. Journal of Bacteriology3 (1982): 1397-1402.
- Jensen WB. “The origin of the Soxhlet extractor”. Journal of Chemical Education12 (2007): 1913.
- Jiang Q. “Natural forms of vitamin E: metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy”. Free Radical Biology and Medicine 72 (2014): 76-90.
- Kanikireddy V., et al. “Carboxymethyl cellulose-based materials for infection control and wound healing: A review”. International Journal of Biological Macromolecules 164 (2020): 963-975.
- Knecht K., et al. “Tocopherol and tocotrienol analysis in raw and cooked vegetables: a validated method with emphasis on sample preparation”. Food Chemistry 169 (2015): 20-27.
- Lee GY and Han SN. “The role of vitamin E in immunity”. Nutrients11 (2018): 1614.
- Lee IM., et al. “Vitamin E in the primary prevention of cardiovascular disease and cancer: the Women’s Health Study: a randomized controlled trial”. JAMA1 (2005): 56-65.
- Lee WL., et al. “Does a simple hematological examination predict the response and side effects in patients undergoing induction chemotherapy and/or neoadjuvant chemotherapy?” Journal of the Chinese Medical Association2 (2020): 107-108.
- Mène-Saffrané L. “Vitamin E biosynthesis and its regulation in plants”. Antioxidants1 (2017): 2 (2017): 375-392.
- Naguib MM and Valvano MA. “Vitamin E increases antimicrobial sensitivity by inhibiting bacterial lipocalin antibiotic binding”. Msphere6 (2018): e00564-618.
- Neophytou CM and Constantinou AI. “Drug delivery innovations for enhancing the anticancer potential of vitamin E isoforms and their derivatives”. BioMed Research International (2015).
- Norris SR., et al. “Complementation of the Arabidopsis pds1 mutation with the gene encoding p-hydroxyphenylpyruvate dioxygenase”. Plant Physiology4 (1998): 1317-1323.
- Ozsoz M., et al. “Analysis Tocopherol Using Chromatographic and Electrochemical Techniques”. Vitam Miner186 (2019): 2.
- Qi N., et al. “Simultaneous analysis of eight vitamin E isomers in Moringaoleifera Lam. leaves by ultra-performance convergence chromatography”. Food Chemistry 207 (2016): 157-161.
- Raederstorff D., et al. “Vitamin E function and requirements in relation to PUFA”. British Journal of Nutrition8 (2015): 1113-1122.
- Reiter E., et al. “Anti-inflammatory properties of α-and γ-tocopherol”. Molecular Aspects of Medicine5-6 (2007): 668-691.
- Rigotti A. “Absorption, transport, and tissue delivery of vitamin E”. Molecular Aspects of Medicine5-6 (2007): 423-436.
- Ruggeri BA., et al. “Effects of low-temperature acclimation and oxygen stress on tocopheron production in Euglena gracilis Z”. Applied and Environmental Microbiology6 (1985): 1404-1408.