Abstract

The present study describes the ability to degrade biodegradable plastics by bacteria isolated from sludge and soil. The content of plastic are carbon, hydrogen, silicon, oxygen, chloride, nitrogen and are derived from different sources such as coal, oil, and natural gas. Biodegradable plastic was buried in the glass bottle with three layers of different soil like Rhizospheric soil, Sludge and Garden soil for four months. The bacteria was then isolated and identified as Micrococcus sp. VC4 on the basis of biochemical studies. The bacteria Micrococcus sp. VC4 breaks the polymers and used as sole carbon source for their metabolic activities was estimated by FTIR analysis and Strum Test.

Keywords: Hydrocarbons; Biodegradable Plastic; FTIR Analysis; Bushnell’s Broth; Strum Test

References

1. Sangale MK., et al. "A review on Biodegradation of Polythene: The microbial approach”. Journal of Bioremediation and Biodegradation 3 (2012): 164.
2. Mahdiyah Dede and B H Mukti. "Isolation of polyethylene plastic degrading-bacteria”. Bioscience International2 (2013): 29-32.
3. Ali Muhammad Ishtiaq., et al. "Studies on biodegradation of cellulose blended polyvinyl chloride films”. International Journal of Agriculture and Biology 5 (2009): 577-580.
4. Shah Ziaullah., et al. "Degradation of polyester polyurethane by an indigenously developed consortium of Pseudomonas and Bacillus species isolated from soil”. Polymer Degradation and Stability134 (2016): 349-356.
5. Christian Venisha V., et al. "Biodegradation of Polythene by Novel Bacteria Sphingomonas sp. VC1”. International Research Journal of Engineering and Technology 8 (2020).
6. Christian Amita Mishra and Meenu Saraf. “Study of Bacteria Isolated from Biodegradable Polythene”. Bioscience Biotechnology Research Communications1 (2020): 204-209.
7. Chua Adeline SM., et al. "Production of polyhydroxyalkanoates (PHA) by activated sludge treating municipal wastewater: effect of pH, sludge retention time (SRT), and acetate concentration in influent”. Water Research15 (2003): 3602-3611.
8. Dave H., et al. "Biodegradation of starch–polyethylene films in soil and by microbial cultures”. World Journal of Microbiology and Biotechnology6 (1997): 655-658.
9. Hossain Khandkar Shaharina., et al. "Isolation and characterization of polythene degrading bacteria from garbage soil”. International Journal of Agriculture, Environment and Bioresearch 4 (2019).
10. Jain Roopesh., et al. "Polyhydroxyalkanoates: a way to sustainable development of bioplastics”. Chronicles of Young Scientists3 (2010): 10-10.
11. Jeol FR. “Polymer Science and Technology: Introduction to polymer science”. Eds. 3, Pub: Prentice hall PTR lnc., Upper Saddle River, New Jersey 07458 (1995): 4-9.
12. Koutny Marek., et al. "Biodegradation of polyethylene films with prooxidant additives”. Chemosphere8 (2006): 1243-1252.
13. Kumar Santosh., et al. "Isolation and identification of LDPE degrading fungi from municipal solid waste”. Journal of Chemical and Pharmaceutical Research3 (2013): 78-81.
14. Premraj R and Mukesh Doble. "Biodegradation of polymers”. Indian Journal of Biotechnology2 (2005): 186-193.
15. Rudel Ruthann A., et al. "Chemicals causing mammary gland tumors in animals signal new directions for epidemiology, chemicals testing, and risk assessment for breast cancer prevention”. Cancer: Interdisciplinary International Journal of the American Cancer Society109 (2007): 2635-2666.
16. Shah Aamer Ali., et al. "Degradation of polyurethane by novel bacterial consortium isolated from soil”. Annals of Microbiology3 (2008): 381.