Bing Tang^1,2,3*, Jung-Feng Hsieh⁴, Chichen Michael Chien^5,6,7 and Shui-Tein Chen^1,8,9

¹Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

*Corresponding Author: Bing Tang, Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.

Received: August 26, 2021; Published: August 28, 2021

Abstract

Objective: The immuno-modulating activity of natural substances was evaluated to assess their effects on human umbilical cord blood mononuclear cells (MNC). Most of the MNC might have been immunologically primed as they are exposed to immunological stressors, while MNC from the human umbilical cord blood (hUCB) are not. During pregnancy, MNC of hUCB is isolated from bio-stressors by virtue of the trophoblast stratum between the placenta and its neighboring maternal endometrium. Hence, the MNC is well protected from bio-stresses. The trophoblastic layer prevents MNCs of the hUBC from being affected by various factors circulating in the maternal blood, so that the exposure of MNC to those factors is reduced.

Methods: After treatment with eleven different natural substances, including five herbal extracts and six pure compounds, the immunophenotypic expression of MNC subsets was analyzed via fluorescence activated cell sorting (FACS) analysis. Data reported here indicated that the extract wheat grass (Triticum aestivum L.) intensified the population of CD56+ natural-killer (NK) cells, whereas the propagation of other MNC was insignificant.

Results: NK cells separated with the positive magnetic bead cell isolation method (MACS) and treated with wheat grass extract showed marked cytotoxicity affecting the cells of Modal karyotype of human leukemia cell line, K562.

Conclusions: This pilot study shows that wheat grass extracts likely orchestrates, enforces, and promotes the in vitro maturation of CD56⁺ NK cells in the hUCB. (Med J South Taiwan 2011;7:79-93).

Keywords: CD56+; Wheat; NK Cells

Bibliography

1. Nociari MM., et al. “A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity”. Journal of Immunology Methods 213 (1998): 157-167.
2. Joshi SS., et al. “Antitumor activity of human umbilical cord blood cells: A comparative analysis with peripheral blood and bone marrow cells”. International Journal of Oncology 13 (1998): 791-9.
3. Warren HS., et al. “Functional inhibitory human leucocyte antigen class I receptors on natural killer (NK) cells in patients with chronic NK lymphocytosis”. British Journal of Haematology 121 (2003): 793-804.
4. Chien CM., et al. “Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56+ NK-cell cytotoxicity in cord blood”. Bioorganic and Medicinal Chemistry 12 (2004): 5603-5609.
5. Wang YY., et al. “Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities”. Bioorganic and Medicinal Chemistry 10 (2002): 1057-1062.
6. Gritzapis AD., et al. “Large-scale expansion of CD3 (+)CD56 (+) lymphocytes capable of lysing autologous tumor cells with cytokine- rich supernatants”. Cancer Immunology and Immunotherapy 51 (2002): 440-448.
7. Roehm NW., et al. “An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT”. Journal of Immunology Methods 142 (1991): 257-265.
8. Scudiero DA., et al. “Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines”. Cancer Research 48 (1998): 4827-4833.
9. Zhang T and Sentman CL. “Cancer immunotherapy using a bispecific NK receptor fusion protein that engages both T cells and tumor cells”. Cancer Research 71 (2011): 2066-2076.
10. Andriani P and Mckelvey B. “From skew deviation to power-law science”. In: Allen P, Maguire S, editors. Sage Handbook of Complexity an Management. London: Sage; (2011): 254-273.
11. Andriani P and McKelvey B. “Using scale-free theory from complexity science to better management risk”. Risk Management, An International Journal 12 (2010): 54-82.
12. Klersy C., et al. “Advantages of the lognormal approach to determining reference change values for N- terminal propeptide B-type natriuretic peptide”. Clinical Chimica Acta (2011).
13. Huang Y., et al. “Interleukin- 12 treatment down-regulates STAT4 and induces apoptosis with increasing ROS production in human natural killer cells”. Journal of Leukocyte Biology 90 (2011): 87-97.
14. Yamaguchi Y., et al. “Postoperative immunosuppression cascade and immunotherapy using lymphokine- activated killer cells for patients with esophageal cancer: possible application compensatory anti-inflammatory response syndrome”. Oncology Report 15 (2006): 895-901.

Citation

Citation: Bing Tang., et al. “A Study: On in Vitro Maturation of CD56+ NK Cells Using the Human Umbilical Cord Blood, Among the Eleven Natural Nutritional Substances Tested, Wheat Grass Extract Orchestrates and Enforces the Naive Fate of CD56+ NK Cells”. Acta Scientific Computer Sciences 3.6 (2021): 10-20.

Copyright

Copyright: © 2021 Bing Tang., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.