Acta Scientific Biotechnology

Research Article Volume 2 Issue 6

A Study: On in Vitro Maturation of CD56+ NK Cells Using the Human Umbilical Cord Blood, Among the Eleven Natural Nutritional Substances Tested, Wheat Grass Extract Orchestrates and Enforces the Naive Fate of CD56+ NK Cells

Bing Tang1,2,3*, Jung-Feng Hsieh4, Chichen Michael Chien5,6,7 and Shui-Tein Chen1,8,9

1Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

2Research and Ethics, Danville, California, USA

3Research Consultant Emeritus, New York College of Traditional Chinese Medicine, Mineola, N.Yt USA

4Department of Food Science, Fu Jen Catholic University, New Taipei City, Taiwan

5Hsinchu Science Park Employeesr Clinic, Taiwan

6Hsinchu County Ton-Yen General Hospital, Taiwan

7Institute of Life Sciences, National TsingHia University, Taiwan

8Institute of Biological Chemistry, National Taiwan University, Taipei, Taiwan

9The Genomics Research Center, Academia Sinica, Taipei, Taiwan

*Corresponding Author: Bing Tang, Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.

Received: August 26, 2021; Published: August 28, 2021


Objective: The immuno-modulating activity of natural substances was evaluated to assess their effects on human umbilical cord blood mononuclear cells (MNC). Most of the MNC might have been immunologically primed as they are exposed to immunological stressors, while MNC from the human umbilical cord blood (hUCB) are not. During pregnancy, MNC of hUCB is isolated from bio-stressors by virtue of the trophoblast stratum between the placenta and its neighboring maternal endometrium. Hence, the MNC is well protected from bio-stresses. The trophoblastic layer prevents MNCs of the hUBC from being affected by various factors circulating in the maternal blood, so that the exposure of MNC to those factors is reduced.

Methods: After treatment with eleven different natural substances, including five herbal extracts and six pure compounds, the immunophenotypic expression of MNC subsets was analyzed via fluorescence activated cell sorting (FACS) analysis. Data reported here indicated that the extract wheat grass (Triticum aestivum L.) intensified the population of CD56+ natural-killer (NK) cells, whereas the propagation of other MNC was insignificant.

Results: NK cells separated with the positive magnetic bead cell isolation method (MACS) and treated with wheat grass extract showed marked cytotoxicity affecting the cells of Modal karyotype of human leukemia cell line, K562.

Conclusions: This pilot study shows that wheat grass extracts likely orchestrates, enforces, and promotes the in vitro maturation of CD56+ NK cells in the hUCB. (Med J South Taiwan 2011;7:79-93).

Keywords: CD56+; Wheat; NK Cells


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Citation: Bing Tang., et al. “A Study: On in Vitro Maturation of CD56+ NK Cells Using the Human Umbilical Cord Blood, Among the Eleven Natural Nutritional Substances Tested, Wheat Grass Extract Orchestrates and Enforces the Naive Fate of CD56+ NK Cells”. Acta Scientific Computer Sciences 3.6 (2021): 10-20.


Copyright: © 2021 Bing Tang., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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