Acta Scientific Biotechnology

Research Article Volume 2 Issue 3

Spectrophotometric and HPLC Estimation of Total Alkaloids of Citrus limon L. Burm. f. (Lemon) and its Endophytes

Rathod Zalak R1 and Saraf Meenu S2*

1Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, India
2Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, India

*Corresponding Author: Saraf Meenu S, Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, India.

Received: April 07, 2021; Published: May 29, 2021

Abstract

Citrus limon (Lemon) belongs to Family Rutaceae, is commonly known as Kagdi Limbu or Lime. The current examination was to decide the total alkaloid content in Citrus limon plant and its endophytes isolated of leaf, steam, root, seed and peel. The total alkaloid content (TAC) was assessed spectrophotometrically utilizing Bromocresol green strategy and HPLC with caffeine as standard. rich wellspring of alkaloids. Among the different parts examinations leaf extracts showed higher alkaloids. The total alkaloid content of different Endophytes isolated from whole Citrus limon plant with respect to extract of Citrus limon plant was well established by spectrophotometric and HPLC studies. The near assessment of all out alkaloid in the Endophytes was accomplished interestingly which can be utilized for additional synthetic and natural examinations and unrefined medication material in planning of home grown definition.

Keywords: Alkaloids; Caffein; Citrus limon (Lemon); HPLC; Spectrophotometer

Citation

Citation: Rathod Zalak R and Saraf Meenu S. “Spectrophotometric and HPLC Estimation of Total Alkaloids of Citrus limon L. Burm. f. (Lemon) and its Endophytes”. Acta Scientific Biotechnology 2.3 (2021): 21-26.

Copyright

Copyright: © 2021 Rathod Zalak R and Saraf Meenu S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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