Acta Scientific Biotechnology

Short Communication Volume 1 Issue 10

Enzyme Promiscuity and Molecular Mechanism; Mystery Remain Unsolved

Mahendra Kumar Verma1* and Samba Naik A2

1Scientist, Centre for Molecular Biology Research (CMBR) Bhopal, Madhya Pradesh, India
2Lecturer, Department of Zoology, KBN College, Vijayawada, Andhra Pradesh, India

*Corresponding Author: Mahendra Kumar Verma, Scientist, Centre for Molecular Biology Research (CMBR) Bhopal, Madhya Pradesh, India.

Received: September 17, 2020; Published: September 30, 2020

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  Enzymes/proteins are the most versatile bio-molecules in the living system. In the living system, the enzyme catalyzes biochemical reactions in varying magnitude. The nature of the enzyme can be defined as robust and effective. It is believed enzymes are highly specific towards its substrate. At the same enzyme do catalyze multiple biochemical reactions at varying pace. There is growing research evidence suggests enzymes are highly promiscuous and capable of multiple binding substrates. Promiscuity is a phenomenon where enzymes do develop an affinity for various substrates and catalyze numerous biochemical reactions. Further, promiscuity is a natural phenomenon that can be at the substrate level and or at the catalytic level. However, recent studies have established artificial promiscuity called induced promiscuity. Induced promiscuity is entirely different from protein engineering, where a specific amino acid/s modified for the desired property. Over the last decade, growing research work was carried out to understand the nature and mechanism of enzyme promiscuity along with its commercial implication. Despite extensive research work on enzyme promiscuity still, we are unable to comprehend molecular insights of enzyme promiscuity. As a core area of green chemistry, enzyme promiscuity is crucial not only for the extended use of such unique molecules but also for sustainable development as well. The wide range of applications of enzymes, including healthcare, agriculture, industry, and food industry, the enzyme promiscuity had shown tremendous scope in the future.

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References

  1. Martínez-Núñez MA., et al. “Tracing the Repertoire of Promiscuous Enzymes along the Metabolic Pathways in Archaeal Organisms”. Life3 (2017): 30.
  2. Gupta R. “Recent advances in enzyme promiscuity”. Sustainable Chemical Process 4 (2016): 2.
  3. Hult K and Berglund P. “Enzyme promiscuity: Mechanism and applications”. Trends in Biotechnology 25 (2007): 231-238.
  4. Peregrin-Alvarez J.M., et al. “The phylogenetic extent of metabolic enzymes and pathways”. Genome Research 13 (2003): 422-427.
  5. Arora B., et al. “Enzyme promiscuity: Using the dark side of enzyme specificity in white biotechnology”. Sustainable Chemistry and Pharmacy 2 (2014): 25.
  6. Lamble HJ., et al. “Metabolic pathway promiscuity in the archaeon Sulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase”. Journal of Biological Chemistry 278 (2003): 34066-34072.
  7. Verma MK and Pulicherla KK. “Broad substrate affinity and catalytic diversity of fibrinolytic enzyme from Pheretima posthumous-Purification and molecular characterization study”. International Journal of Biological Macromolecules 95 (2017): 1011-1021.
  8. Verma MK and Pulicherla KK. “Enzyme promiscuity in Earthworm serine protease- Substrate versatility and therapeutic potential”. Amino Acid 4 (2016): 941-948.
  9. Jia B., et al. “Multifunctional enzymes in archaea: promiscuity and moonlight”. Extremophiles2 (2013): 193-203.
  10. Khersonsky O and Tawfik DS. “Enzyme promiscuity: a mechanistic and evolutionary perspective”. Annual Review of Biochemistry 79 (2010): 471-505.
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Citation

Citation: Mahendra Kumar Verma and Samba Naik A. “Enzyme Promiscuity and Molecular Mechanism; Mystery Remain Unsolved". Acta Scientific Biotechnology 1.10 (2020): 24-25.




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