Acta Scientific Biotechnology (ASBT)

Research Article Volume 1 Issue 7

Genetic Diversity Analysis among Garlic (Allium sativum L.) Genotypes through Molecular Markers

Shekhar Mokate, Maithilee Kesralikar*, DN Vakharia and BA Golakiya

Department of Biotechnology, College of Agriculture, Junagadh, Gujarat, India

*Corresponding Author: Maithilee Kesralikar, Department of Biotechnology, College of Agriculture, Junagadh, Gujarat, India.

Received: May 25, 2020; Published: June 10, 2020

×

Abstract

 An experimental research was conducted among the 16 garlic genotypes for analysis of genetic diversity. The present investigation on “Genetic diversity analysis among garlic (Allium sativum L.) genotypes through molecular markers” was planned to conduct with objective of DNA fingerprinting through RAPD, ISSR and SSR primers. RAPD ISSR and SSR markers are used for DNA fingerprinting. Among all markers ISSR and SSR markers gave diversified results than RAPD.

Keywords: Allium sativum L; Genetic Diversity Analysis; Genotypes; Molecular Markers

×

References

  1. Thompson HC and Kelly WC. “Vegetable crops”. Mcgraw Hill Company Ltd., Bombay, India (1957): 368-370.
  2. Mangum P and Peffley EB. “Inheritance of ADH, 6-PGDH, PGM, and SKDH in Allium fistulosum L”. Journal of the American Society for Horticulture Science2 (1994): 335-338.
  3. Williams J., et al. “DNA polymorphisms amplified by random primers are useful as genetic markers”. Nucleic Acids Research 22 (1990): 6531-6535.
  4. Tingey SV., et al. “Genetic analysis with RAPD markers”. Proceedings of the symposium: Applications of RAPD technology to Plant Breeding. Minneapolis, MN (1992).
  5. Ragot M and Hoisington DA. “Molecular markers for plant breeding: Comparison of RFLP and RAPD genotype costs”. Theoretical and Applied Genetics 86 (1993): 975-984.
  6. Smith ND and Devey ME. “Occurrence and inheritance of microsatellites in Pinus radiata”. Genomics6 (1994): 977-983.
  7. Vosman MI., et al. “Abundance, polymorphism and genetic mapping of microsatellites in Allium”. Plant Breeding 45 (1992): 156-167.
  8. Doyle JJ and Doyle JL. “A rapid DNA isolation procedure for small quantities of fresh leaf tissue”. Phytochemistry Bulletin 19 (1987): 11-15.
  9. Al-Otayk S., et al. “Variation in productive characteristics and diversity assessment of garlic cultivars and lines using DNA markers”. JKAU: Meteorology, Environment and Arid Land Agriculture 20 (2008): 63-79.
  10. Mahajan V., et al. “Genetic fingerprinting of onion cultivars using SSR markers”. Indian Journal of Horticulture 66 (2009): 62-68.
  11. Rohlf FJ. “Numerical taxonomy and multivariate analysis system version 1.80 manual”. Exeter Software, New York (1994).
  12. Abdoli M., et al. “Classification of Iranian garlic (Allium sativum) ecotype using RAPD marker”. Journal of Medicinal Plants 8 (2009): 45-51.
  13. Lee H., et al. “Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique”. Genome3 (2003): 514-520.
  14. Peffley EB and Currah L. “The chromosomal locations of enzyme-coding genes Adh-I and Pgm-1 in Allium fistulosum L”. Theoretical and Applied Genetics 75 (1988): 945-949.
×

Citation

Citation: Maithilee Kesralikar., et al. “Genetic Diversity Analysis among Garlic (Allium sativum L.) Genotypes through Molecular Markers Acta Scientific Biotechnology 1.7 (2020): 03-08.




Member In



News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 15, 2020.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of “Best Article of the Issue”.
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.
  • Contact US