Acta Scientific Biotechnology (ASBT)

Research Article Volume 1 Issue 4

Engineered Pseudomonas putida for Biosynthesis of Catechol from Lignin Derived Model Compounds and Biomass Hydrolysate

Priya Upadhyay1 and Arvind Lali1,2*

1DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, Maharashtra, India
2Department of Chemical Engineering, Institute of Chemical Technology, Mumbai, Maharashtra, India

*Corresponding Author: Arvind Lali, DBT-ICT Centre for Energy Biosciences and Department of Chemical Engineering, Institute of Chemical Technology, Mumbai, Maharashtra, India.

Received: March 02, 2020; Published: March 14, 2020

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Abstract

  Catechol is one of the industrially relevant chemicals with myriad applications. Its production via chemical route suffers from several drawbacks the major being a non-green and non-selective route. Currently, bio-based products using biocatalyst are gaining attention due to the growing awareness about the environmental and health hazards over the use of petroleum derived feedstock. Lignocellulosic biomass serves as a promising sustainable and renewable feedstock wherein celluloses have found successful ways to a variety of fuel and biochemical molecules but concomitantly results in surplus lignin being produced. Lignin valorization is the demand of the current scenario to make the cellulosic bio-refineries viable. This task is complicated by complexity, heterogeneity and diversity of lignin structures posing limitations towards lignin valorization via chemical routes. There are several microorganisms that possess the ability to metabolize lignin monomers via their central metabolic pathways and this paves a way to synthesis of a number of products. Pseudomonas putida KT2440 is one such organism and was chosen for genetic manipulations for catechol biosynthesis using lignin derived model compounds and biomass hydrolysate stream comprising of various lignin fragments/monomers. Catechol production was engineered by diverting various lignin monomers and addressing the identified metabolic bottlenecks particularly vanillic acid accumulation towards catechol biosynthesis. The engineered strain could convert the model lignin monomers as well as monomers in the biomass hydrolysates to catechol and vanillic acid in more than 60% and 90% molar yields, respectively.

Keywords: Pseudomonas putida; Catechol; Lignin Valorization; Lignin Derived Model Compounds; Biomass Hydrolysates

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Citation

Citation: Priya Upadhyay and Arvind Lali. “Engineered Pseudomonas putida for Biosynthesis of Catechol from Lignin Derived Model Compounds and Biomass Hydrolysate".Acta Scientific Biotechnology 1.4 (2020): 03-12.




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