Prachi Tripathi and Vinay Kumar Singh*

Malacology Laboratory, Department of Zoology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh-273009, India

*Corresponding Author: Deborah Maigawu Buba, University of Jos, Department of Vinay Kumar Singh, Malacology Laboratory, Department of Zoology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh-273009, India. Cell Phone: +91-9807110100; Email: vinaygkpuniv@ gmail.com; vinay.zool@ddugu.ac.in

Received: October 10, 2026; Published: November 04, 2026

Abstract

Background: Lymnaea acuminata is an important intermediate host of fascioliasis affecting humans and livestock, and controlling it is essential to interrupt disease transmission.

Objective: To evaluate the molluscicidal activity of crude aqueous, ethanolic, and column-purified extracts of different parts of Artemisia annua against L. acuminata under laboratory conditions.

Methods: Adult L. acuminata were exposed to different extract concentrations for 24–96 h, using six aquaria with 10 snails each per concentration. Mortality was recorded, and LC₅₀ values were determined using probit analysis.

Results: Molluscicidal activity varied with plant part, extraction method, and exposure duration. The column-purified leaf extract showed the lowest 96-h LC₅₀ (0.84 mg/L), followed by the artemisinin reference compound (3.37 mg/L), ethanolic leaf extract (7.14 mg/L), and ethanolic flower extract (9.49 mg/L). Among crude aqueous extracts, the leaf extract (417.19 mg/L) showed a lower LC₅₀ than the flower extract (765.61 mg/L). No observable mortality occurred with the tested stem extract at any exposure period.

Conclusion: Artemisia annua, particularly its column-purified leaf extract, demonstrated molluscicidal potential against L. acuminata. Further studies are required to evaluate its environmental safety and practical applicability.

Subtitle: Molluscicides from the Plant Artemisia annua against the Harmful Snail Lymnaea acuminata.

Keywords: Artemisia annua; Lymnaea acuminata; Fascioliasis; Molluscicidal Activity; Plant-Derived Molluscicides; Dose-Response Relationship

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Citation

Citation: Prachi Tripathi and Vinay Kumar Singh. “Molluscicidal Efficacy of Artemisia annua: Influence of Plant Part and Extraction Method Against Lym- naea acuminata Snail". Acta Scientific Veterinary Sciences 8.4 (2026): 23-32.

Copyright

Copyright: © 2026 Prachi Tripathi and Vinay Kumar Singh. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.