Anandha Narayanan S¹*, Suresh S¹, Prashanth D’souza², Deepak Mundkinajeddu² and Vasudha Kuncham³

¹Animal Health Division, Natural Remedies Private Limited, 5B, Veera Sandra Industrial Area, Electronic City Phase-II, Bengaluru, India
²Research and Development Department, Natural Remedies Private Limited, 5B, Veera Sandra Industrial Area, Electronic City Phase-II, Bengaluru, India
³Head of Research and Development Department, Natural Remedies Private Limited, 5B, Veera Sandra Industrial Area, Electronic City Phase-II, Bengaluru, India
⁴Animal Health Science, Research and Development Department, Natural Remedies Private Limited, 5B, Veera Sandra Industrial Area, Electronic City Phase-II, Bengaluru, India

*Corresponding Author: Anandha Narayanan S, Animal Health Division, Natural Remedies Private Limited, 5B, Veera Sandra Industrial Area, Electronic City Phase-II, Bengaluru, India.

Received: February 05, 2025; Published: February 25, 2025

Abstract

Objective: Rumen is a complex ecosystem comprising a wide range of microbial populations which play a vital role in the fermentation process in livestock. However, the fermentation resulted in the production of an excess amount of methane which is responsible for the loss of energy and environmental pollution. The inclusion of feed additives in the diet may modulate the fermentation as well as the performance of the ruminants. The present study was conducted to evaluate the impact of the Polyherbal formulation (PHF), Kolin Plus on the ruminal fermentation using Rumen Simulation Technique (RUSITEC) culture system.

Materials and Methods: Cattle (n = 4) were fistulated as per ethical guideline to collect rumen digesta. The rumen fluid was placed in the fermenters of RUSITEC system for further analysis. Control group was treated with basal diet. Whereas the Kolin Plus treated groups comprised of addition of 40g and 60g Kolin Plus per 500kg animal per day for 21 days along with basal diet. The parameters like in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), in vitro crude protein digestibility, in vitro crude fiber digestibility, in vitro ether extract digestibility, volatile fatty acid (VFA) production, methane production, ammoniacal nitrogen levels and pH change were assessed.

Result: The results indicated that PHF-Kolin Plus intended for the treatment of indigestion in the ruminants showed a significant effect on ruminal pH, ammoniacal nitrogen, IVDMD, IVOMD, in vitro crude protein digestibility, in vitro crude fiber digestibility, VFA production and methane production.

Conclusion: In conclusion, PHF-Kolin Plus may be considered a promising feed additive that plays an important role in digestion and enhancing the feed efficiency of ruminant livestock.

 Keywords: Polyherbal formulation; RUSITEC; Dry Matter; Crude Protein; Crude Fibre; VFA; IVDMD; IVOMD

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Citation

Citation: Anandha Narayanan S., et al. “Evaluating the Impact of Polyherbal Formulation, Kolin Plus on Ruminal Fermentation Using Rumen Simulation Technique (RUSITEC) Culture System". Acta Scientific Veterinary Sciences 7.3 (2025): 11-20.

Copyright

Copyright: © 2025 Anandha Narayanan S., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.