Eduardo Martínez-Angeles¹, María Gisela Ríos-Durán¹*, Pamela Navarrete-Ramírez², Luciana Raggi ², C Cristian Martínez-Chávez¹ and Carlos A Martínez-Palacios¹

¹Instituto de Investigaciones Agropecuarias y Forestales, Universidad Michoacana de San Nicolás de Hidalgo, México
²Consejo Nacional de Ciencia y Tecnología CONAHCyT, México - Instituto de Investigaciones Agropecuarias y Forestales, Universidad Michoacana de San Nicolás de Hidalgo, México

*Corresponding Author: María Gisela Ríos-Durán, Instituto de Investigaciones Agropecuarias y Forestales, Universidad Michoacana de San Nicolás de Hidalgo, México.

Received: January 23, 2025; Published: February 17, 2025

Abstract

Probiotics have recently been an important supplement in in fish feeds showing positive effects on growth and survival in several freshwater and marine fish species. However, probiotic viability during the inclusion of these supplements in the diets has been a challenge, greatly depending on the elaboration process such as spray-drying for fish larvae microdiets. Importantly, scarce information exists about the influence of this process and microdiet handling conditions on the viability of probiotics, despite its potential to encapsulate and protect these microorganisms, with minimal chemical modification. This study evaluated the effect of the spray-drying process, storage time, and marine water exposure on the viability of Lactobacillus acidophilus encapsulated in microdiets. Spray-dried microdiets that including L. acidophilus La-14, can be stored for up to 6 months at 4 ºC maintaining an adequate CFU count (>10⁹) and can be used as a probiotic supplement in microdiets for marine and freshwater fish larvae. This raises the possibility of implementing new feeding strategies, such as using enriched microdiets with probiotic strains capable of resisting salinity and long-term storage, which can have an essential application for the aquafeed industry.

 Keywords: Microdiet; Lactobacillus Acidophilus; Viability; Fish Larvae

References

1. Yúfera M and MJ Darias. “The onset of exogenous feeding in marine fish larvae”. Aquaculture 268 (2007): 53-63.
2. Hamre K., et al. “Fish larval nutrition and feed formulation: knowledge gaps and bottlenecks for advances in larval rearing”. Reviews in Aquaculture5 (2013): S26-S58.
3. Hu, J., et al. “Feeding and development of warm water marine fish larvae in early life”. Emerging Issues in Fish Larvae Research, edited by M Yúfera, Springer, Cham (2018): 275-296.
4. Blair T., et al. “Evaluation of microdiets versus live feeds on growth, survival and fatty acid composition of larval haddock (Melanogrammus aeglefinus)”. Aquaculture225 (2003): 451-461.
5. Conceição LE., et al. “Live feeds for early stages of fish rearing”. Aquaculture Research41(2010): 613-640.
6. Alam MS., et al. “Growth performance, survival and body composition of southern flounder Paralichthys lethostigma larvae fed different formulated microdiets”. Aquaculture Research46 (2015): 1924-1936.
7. Campoverde C., et al. “Early weaning in meagre Argyrosomus regius: Effects on growth, survival, digestion and skeletal deformities”. Aquaculture Research48 (2017): 5289-5299.
8. Waiyamitra P., et al. “Probiotics modulate tilapia resistance and immune response against tilapia lake virus infection”.  Pathogens9 (2020): 919.
9. Taoka Y., et al. “Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system”. Fisheries Science72 (2006): 310-321.
10. Al‐Dohail MA., et al. “Effects of the probiotic, Lactobacillus acidophilus, on the growth performance, haematology parameters and immunoglobulin concentration in African Catfish (Clarias gariepinus, Burchell 1822) fingerling”. Aquaculture Research 40 (2009): 1642-1652.
11. Wang Y. “Use of probiotics Bacillus coagulans, Rhodopseudomonas palustris and Lactobacillus acidophilus as growth promoters in grass carp (Ctenopharyngodon idella) fingerlings”. Aquaculture Nutrition 17 (2011): 372-378.
12. Salaghi Z, Imanpuor M and V Taghizadeh. “Effect of different levels of probiotic primalac on growth performance and survival rate of Persian sturgeon (Acipenser persicus)”. Global Veterinaria11 (2013): 238-242.
13. Lara-Flores M and MA Olvera-Novoa. “The use of lactic acid bacteria isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), as growth promoters in fish fed low protein diets”. Latin American Journal of Aquatic Research41(2013): 490-497.
14. Rodrigues JB., et al. “High protein microparticles produced by ionic gelation containing Lactobacillus acidophilus for feeding pacu larvae”. Food research international 63 (2014): 25-32.
15. Hoseinifar SH., et al. “Probiotic, prebiotic and synbiotic supplements in sturgeon aquaculture: a review”. Reviews in Aquaculture8 (2016): 89-102.
16. Akter MN., et al. “Effect of Lactobacillus acidophilus supplementation on growth performances, digestive enzyme activities and gut histomorphology of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) juveniles”. Aquaculture Research 50 (2019): 786-797.
17. Dawood MA., et al. “Probiotic application for sustainable aquaculture”. Reviews in Aquaculture11 (2019): 907-924.
18. Liu W., et al. “Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains”. Fish and Shellfish Immunology35 (2013): 54-62.
19. Gebara C., et al. “Viability of Lactobacillus acidophilus La5 in pectin-whey protein microparticles during exposure to simulated gastrointestinal conditions”. Food Research International51 (2013): 872-878.
20. Todorov SD., et al. “Bacteriocin production and resistance to drugs are advantageous features for Lactobacillus acidophilus La-14, a potential probiotic strain”. New Microbiologica34 (2011): 357-370.  
21. Villamil L, Reyes C and MA Martínez‐ “In vivo and in vitro assessment of Lactobacillus acidophilus as probiotic for tilapia (Oreochromis niloticus, Perciformes: Cichlidae) culture improvement”. Aquaculture Research45 (2014): 1116-1125.
22. Martínez-Angeles E., et al. “Effects of formulated microdiets on weaning success and larval performance of pike silverside Chirostoma estor (Jordan, 1879)”. Animal Feed Science and Technology288 (2022): 115288.
23. Tripathi MK and SK Giri. “Probiotic functional foods: Survival of probiotics during processing and storage”.  Journal of Functional Foods 9 (2014): 225-241.
24. Terpou A., et al. “Probiotics in food systems: Significance and emerging strategies towards improved viability and delivery of enhanced beneficial value”. Nutrients11 (2019): 1591.
25. Broeckx G., et al. “Enhancing the viability of Lactobacillus rhamnosus GG after spray drying and during storage.” International Journal of Pharmaceutics 534 (2017): 35-41.
26. Paéz R., et al. “Effect of heat treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion”. Food Research International48 (2012): 748-754.
27. Soukoulis C., et al. “Impact of milk protein type on the viability and storage stability of microencapsulated Lactobacillus acidophilus NCIMB 701748 using spray drying”. Food and Bioprocess Technology 7 (2014): 1255-1268.
28. Rajam R., et al. “Effect of whey protein-alginate wall systems on survival of microencapsulated Lactobacillus plantarum in simulated gastrointestinal conditions”. Journal of Functional Foods4 (2012): 891-898.
29. Khem S, Small DM and BK May. “The behaviour of whey protein isolate in protecting Lactobacillus plantarum”. Food Chemistry 190 (2016): 717-723.
30. Kiekens S., et al. “Impact of spray‐drying on the pili of Lactobacillus rhamnosus GG”. Microbial Biotechnology12 (2019): 849-855.
31. Hai NV. “The use of probiotics in aquaculture.” Journal of Applied Microbiology 119 (2015): 917-935.
32. Wang A., et al. “Use of probiotics in aquaculture of China-a review of the past decade”. Fish and Shellfish Immunology86 (2019): 734-755.

Citation

Citation: María Gisela Ríos-Durán., et al. “Unaltered Viability of Lactobacillus Acidophilus La-14 during Storage and After Marine Water Exposure in Fish Larvae Spray-Dried Microdiets". Acta Scientific Veterinary Sciences 7.3 (2025): 06-10.

Copyright

Copyright: © 2025 María Gisela Ríos-Durán., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.