Bhoj R Singh¹*, Dharmendra K Sinha¹, Karam P Singh² and Abhijit M Pawde³

¹Principal Scientist and Head of Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, India
²Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar, India
³Referral Veterinary Polyclinic, ICAR-Indian Veterinary Research Institute, Izatnagar, India

*Corresponding Author: Bhoj R Singh, Principal Scient and Head of Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, India.

Received: January 22, 2024Published: February 25, 2024

Abstract

Infectious reproductive disorders including abortions and metritis are important hurdles towards economic dairy farming. This study was undertaken to determine infectious bacterial causes of metritis and abortions in buffaloes and determination of their antimicrobial susceptibility. Samples of stomach contents of 23 aborted foeti and uterine aspirates of 20 buffaloes suffering from metritis were received for bacteriological analysis from Referral Veterinary Polyclinic and Centre for Animal Disease Research and Diagnosis. To identify the causal bacteria standard bacteriological method for isolation and identification of bacteria and antimicrobial susceptibility assay of isolates were followed. From 13 cases of abortion only single type of bacteria (Aeromonas media 1, Alcaligenees faecalis 2, Brucella abortus 4, Enterobacter agglomerans 1, Escherichia coli 3, Klebsiella pneumoniae ssp. pneumoniae 1, K. varians1) was detected while in 10 samples more than one type of bacteria were isolated. Antibodies in high titres (≥40) were detected in serum samples of 17 aborting buffaloes showing possibility of systemic infection before abortion. Antibodies were detected against A. media (1), A. faecalis (1), B. abortus (4), E. agglomerans (2), Enterobacter amnigenus (1), E. coli (3), K. pneumoniae ssp. pneumoniae (2), Kocuria varians (1), Salmonella enterica ssp. enterica ser Dublin (1) and Xenorhabdus poinarii (1). From uterine aspirates of six animals with metritis single type of bacteria (Acinetobacter lwoffii 3, Aeromonas salmonicida ssp. salmonicida 1, E. coli 1, K. varians 1) was detected while 14 samples had more than one type of bacteria. Irrespective of source, >90% bacterial isolates were susceptible to ajowan oil, cinnamon oil, cinnamaldehyde, carvacrol and thyme oil. Tigecycline was one of the most effective antibiotics but for other antibiotics susceptibility varied with source and type of bacteria. A total of 31.58% bacteria associated with metritis were resistant to carbapenems and were more often resistant to other antibiotics (MRI=0.45) than isolates from aborted foetuses (MRI= 0.33). Though B. abortus and E. coli were main causes of abortions, there was no fixed set of bacteria causing metritis and abortions in buffaloes. Multiple drug-resistant (MDR) and carbapenem drug-resistant (CR) bacteria are more often associated with metritis than with abortions.

Keywords: Aeromonas Media, Alcaligenees Faecalis, Enterobacter Agglomerans, Kocuria Varians, Micrococcus Vaians, Salmonella Dublin, Xenorhabdus Poinarii.

References

1. Sharma H. “Explained: Reading the livestock census”. The Indian Express.
2. Singh VP and Kaur I. “Economics of Buffalo in Livestock Production System in Punjab: Current Status and Future Prospectus”. International Journal of Current Microbiology and Applied Science10 (2018): 2702-2708.
3. Naveena BM and Kiran M. “Buffalo meat quality, composition, and processing characteristics: Contribution to the global economy and nutritional security”. Animal Frontiers4 (2014): 18-24.
4. Agricultural and Processed Food Products Export Development Authority APEDA. 2021”. Animal Products (2016).
5. Singh NP., et al. “Prevalence of various diseases and health disorders in Murrah buffaloes at organized herd of hot sub-humid eco-region”. The Indian Journal of Animal Sciences5 (2017): 5579-580.
6. Maherchandani S., et al. “Infectious Causes of bubaline abortions In: Bubaline Theriogenology, Purohit G.N. (Ed.)”. International Veterinary Information Service, Ithaca NY (2018).
7. Kumar A., et al. “Non-infectious causes of bubaline abortions. In: Bubaline Theriogenology, Purohit G.N. (Ed.)”. International Veterinary Information Service, Ithaca NY (2015).
8. Galiero G. “Causes of infectious abortion in the Mediterranean buffalo”. Italian Journal of Animal Science2 (2007): 194-199.
9. Satish SK., et al. “Current status of abortion in buffalo (Bubalus bubalis) associated with infectious agents: A short communication”. Journal of Entomology and Zoology Studies 1 (2019): 1328-1334.
10. Azawi OI., et al. “A study of endometritis causing repeat breeding of cycling iraqi buffalo cows”. Reproduction in Domestic Animals6 (2008): 735-743.
11. Azawi OI. “Pathogenesis of postpartum metritis in buffaloes: A review”. Buffalo Bulletin 1 (2013): 1-11.
12. Kumari S., et al. “Risk Factors for metritis and its effect on productive and reproductive performance in dairy cattle and buffaloes”. Agriculture Research 5 (2016): 72-80.
13. Singh BR. “Labtop for Microbiology Laboratory”. Lambert Academic Publishing: Germany. 2009.
14. Carter GR. “Diagnostic Procedures in Veterinary Microbiology, 2^nd edition”. Charles C Thomas Publishers, Springfield (1975).
15. Holt JG., et al. “Bergey's Manual of Determinative Bacteriology, 9^th edition”. Williams and Wilkins, Baltimore, MD (1994).
16. “Brucellosis, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019)”. Chapter 3.1.4
17. 17.    CLSI. M45. “Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, 3^rd edition”. Clinical and Laboratory Standards Institute, Wayne, USA (2015).
18. “Performance Standards for Antimicrobial Disk Susceptibility Tests, 24th Informational Supplement, Document M100-S24 and M11-A8”. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania (2014).
19. Singh BR. “Evaluation of antibacterial activity of Salvia officinalis [L.] Sage oil on veterinary clinical isolates of bacteria”. Noto-are 15782463: Medicine (2013).
20. Azawi OI., et al. “A study on postpartum metritis in Iraqi buffalo cows: bacterial causes and treatment”. Reproduction in Domestic Animals5 (2008): 556-565.
21. Ntirandekura J., et al. “Association of brucellosis with abortion prevalence in humans and animals in Africa: A review”. African Journal of Reproductive Health/La Revue Africaine De La Santé Reproductive 3 (2018): 120-136.
22. Singh BR., et al. “Antimicrobial susceptibility pattern of Brucella isolates from abortion cases in animals in Northern India”. Austin Journal of Veteterinary Science and Animal Husbandry 6.3 (2020): 1062.
23. Hezil Dj., et al. “Salmonella Dublin associated with abortion in dairy cattle in Algiers and comparison of different diagnostic methods”. African Journal of Clinical and Experimental Microbiology 2 (2021): 211-222.
24. Hinton M. “Salmonella dublin abortion in cattle. II. Observations on the whey agglutination test and the milk ring test”. Journal of Hygiene (London)3 (1973): 471-479.
25. Singh BR., et al. “Evaluation of guinea pig model for experimental Salmonella serovar Abortusequi infection in reference to infertility”. Indian Journal of Experimental Biology 40 (2002): 296-303.
26. Singh BR., et al. “Outbreak of abortions and infertility in thoroughbred mares associated with waterborne Aeromonas hydrophila”. Indian Journal of Microbiology 2 (2011): 212-216.
27. Wolf-Jäckel GA., et al. “Diagnostic studies of abortion in Danish cattle 2015-2017”. Acta Veterinaria Scandinavia 62 (2020):
28. Henker LC., et al. “Fibrinonecrotic placentitis and abortion associated with Pantoea agglomerans infection in a mare”. Journal of Equine Veterinary Science 92 (2020):
29. Singh BR., et al. “An outbreak of equine abortion due to lecithinolytic Enterobacter agglomerans (Pantoea agglomerans)”. Intas Polivet2 (2014): 319-322.
30. Singh BR., et al. “Antimicrobial Susceptibility of Rare Enterobacteriaceae Causing Clinical Infections”. Technical Report No (2020). Clin/Epid/ICAR-IVRI/02/2021.
31. Wolf-Jäckel GA., et al. “Bovine abortions revisited-enhancing abortion diagnostics by 16S rDNA amplicon sequencing and fluorescence in situ hybridization”. Frontiers in Veterinary Science 8 (2021):
32. Kandi V., et al. “Emerging bacterial infection: Identification and clinical significance of Kocuria species”. Cureus8 (2016): e731.
33. Tsai CY., et al. “Kocuria varians infection associated with brain abscess: a case report”. BMC Infectious Disease 10 (2010):
34. Singh BR., et al. “Aerobic mesophilic bacterial flora of vagina of healthy Mithun (Bos frontalis)”. Journal of Genital System and Disorders3 (2014): 1-7.
35. Singh BR and Ebibeni N. “Aerobic microbiome of vagina of apparently healthy pregnant large black sows in Nagaland and antimicrobial resistance in isolates”. Global Journal of Pathology and Microbiology (2016):
36. Chen C., et al. “The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases”. Nature Communications 8 (2017):
37. Singh BR and Singh RK. “Nagamese fermented soybean (Axone) reduces age at first parturition, increases litter size and modulates immune response in mouse model”. Journal of Probiotics and Prebiotics 1/2 (2014): 49-54.
38. Nji E., et al. “High prevalence of antibiotic resistance in commensal Escherichia colifrom healthy human sources in community settings”. Science Reports 11 (2021):
39. Purohit MR., et al. “High levels of drug resistance in commensal  coliin a cohort of children from rural central India”. Science Reports 9 (2019): 6682.
40. Singh BR., et al. “Antimicrobial drug resistance pattern of bacteria isolated from cases of abortion and metritis in animals”. In: International Conference on Reproductive Biology and Comparative Endocrinology (ICRBCE) 9-11 February 2017. Department of Animal biology, University of Hyderabad, India (2017): 1-IL-35.
41. Singh BR., et al. “List of Carbapenem (Meropenem or Imipenem or Ertapenem) Resistant Bacteria Isolated at Epidemiology Laboratory of Indian Veterinary Research Institute, Izatnagar, India from April 2011 to August 2017 from Different Animal Sources and their Environment and Phenotypic and Genotypic characterization of the Isolates for Different Carbapenemases”. Technical Report (2017).
42. Bhardwaj M., et al. “Emergence of carbapenemase producing pathogens in animals”. Pharmaceutica Anallytica Acta 6 (2015): 1-6.
43. Vinodhkumar OR., et al. “Multi-drug Resistant (MDR), extended spectrum beta-lactamase (ESBL) and New Delhi metallo beta-lactamase (NDM) producing Escherichia coli in rescued sloth bears (Melursus ursinus), India”. Veterinary Research Communications (2021).
44. Bhardwaj M., et al. “Potential of Herbal Drug and Antibiotic Combination Therapy: A New Approach to Treat Multidrug Resistant Bacteria”. Pharmaceutica Analytica Acta11 (2016): 1-4.
45. Singh BR., et al. “Potential of herbal antibacterials as an alternative to antibiotics for multiple drug resistant bacteria: An analysis”. Research Journal of Veterinary Sciences1 (2020): 1-9.
46. Singh BR., et al. “Antimicrobial resistance profile of enteropathogens isolated from diarrhea patients: Herbal antimicrobials, a ray of hope”. Annals of Pharmacology and Pharmaceutics 13 (2017): 1068-1078.

Citation

Citation: Bhoj R Singh., et al. “Antimicrobial Susceptibility of Bacterial Causes of Abortions and Metritis in Buffaloes (Bubalus bubalis) in Bareilly Region, Uttar Pradesh, India"Acta Scientific Veterinary Sciences 6.3 (2024): 82-92.

Copyright

Copyright: © 2024 Bhoj R Singh., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.