Shaikh SR¹* and Siddiqui MFMF²

¹PhD Scholar, Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Sciences, MAFSU, Parbhani, Maharashtra, India
²Assistant Professor, Department of Veterinary Clinical Medicine, Ethics and Jurisprudence College of Veterinary and Animal Sciences, MAFSU, Parbhani, Maharashtra, India

*Corresponding Author: Shaikh SR, PhD Scholar, Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Sciences, MAFSU, Parbhani, Maharashtra, India.

Received: September 28, 2023 Published: December 10, 2023

Abstract

The present research work was conducted to investigate the epidemiological comparison of some indirect screening tests for diagnosis of sub-clinical mastitis in dairy cows. A total of 2976 quarter milk samples from 744 milking cows in and around Parbhani district from various organized and unorganized farms, gaushalas and local dairy farms were screened for sub-clinical mastitis using modified California mastitis test (MCMT), pH and electrical conductivity (EC) in comparison to somatic cell count (SCC) as a reference standard test. The threshold value of milk SCC, EC and pH were found to be 196.078×103 cells/ml, 4.50 mS/cm and 6.6, respectively in indigenous cows and 239.651×103 cells/ml, 5.25 mS/cm and 6.7 in crossbred cows, for identification of subclinical mastitis quarters. The prevalence of sub-clinical mastitis was found 27.15%, 24.73%, 20.43 and 18.01% detected by using SCC, MCMT, pH and EC, respectively. Out of 2976 quarter milk samples subjected to MCMT, pH and EC, the per cent accuracies were found to be 92.00, 90.15 and 67.23 respectively. The highest sensitivity was found with MCMT (73.26%) while the highest specificity was found with EC (97.22%). Positive predictive value was found highest in EC (80.95%) whereas highest negative predictive value was found in MCMT (94.30%) as compared to other screening test for detection of sub-clinical mastitis in cattle. Apparent prevalence was found 17.84%, 16.30%, 13.51% and 12.01% detected by SCC, MCMT, pH and EC, respectively. The κ values of all the three screening tests were above 0.4 with highest MCMT (0.7183) indicating significant agreement with the gold standard test control. The results showed that MCMT was more reliable and can be used efficiently with SCC test for pinpoint diagnosis of sub-clinical mastitis in dairy bovines under field condition.

Keywords: Epidemiology; Investigation; Screening Test; Diagnosis; Mastitis; Cow

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Citation

Citation: Shaikh SR and Siddiqui MFMF. “Epidemiological Investigation of Some Indirect and Reference Tests for Screening of Bovine Subclinical Mastitis".Acta Scientific Veterinary Sciences 6.1 (2024): 30-37.

Copyright

Copyright: © 2024 Shaikh SR and Siddiqui MFMF. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.