RL Bhagat*, SH Sontakke, HD Kadam, VH Shende and JR Khadse

BAIF Development Research Foundation, Central Research Station, Urulikanchan, Pune, Maharashtra, India

*Corresponding Author: RL Bhagat, BAIF Development Research Foundation, Central Research Station, Urulikanchan, Pune, Maharashtra, India.

Received: August 16, 2023; Published: August 29, 2023

Abstract

The objective of this study was to evaluate the effect of reducing sperm concentration per semen dose in cryopreserved bull semen on the conception rate in artificial insemination technology in dairy animals. For this purpose, semen was collected from six bulls (2 each from HF100%, HF75%, and Murrah buffalo). The ejaculates were split-sampled and packed in 20 million, 15 million, and 10 million spermatozoa per straw. After dilution, the semen was cooled to 4°C, equilibrated for 4 hours, and packaged in French Mini straws. A total of 8,418 inseminations were performed over a period from June 2012 to April 2014. The overall mean conception rate in HF100%, HF75%, and Murrah buffalo was recorded as 57.79 ± 0.83, 58.09 ± 0.94, and 53.63 ± 1.06 percent respectively. Sperm concentration-wise conception rate was recorded as 56.75 ± 0.77, 56.57 ± 1.14 and 57.08 ± 1.01 percent, for 20, 15, and 10 million sperm concentration per straw respectively, non-significant difference in conception rate was noticed on account of different sperm concentrations per straw. In conclusion, the reduction of sperm concentration from existing 20 million to 15 million or 10 million per straw did not affect the fertility of cryopreserved cattle or buffalo bull semen, however, it was felt that more studies are needed to confirm the results before a policy decision is taken in this respect.

Keywords: Sperm Concentration; Cryopreserved Semen; Conception Rate; Artificial Insemination; Hf100%, Hf75%; Murrah Buffalo Bulls

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Citation

Citation: RL Bhagat., et al. “Effect of Reducing Sperm Concentration per Insemination Dose in Cryopreserved Semen on Conception Rate".Acta Scientific Veterinary Sciences 5.9 (2023): 111-113.

Copyright

Copyright: © 2023 RL Bhagat., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.