Polymorphism of Myxovirus Resistance (Mx) Gene in Four Nigerian Chicken Genotypes
Okafor OL1, Godwin CN2, Nelson J2, Nwogu CM1 and Agaviezor BO2*
1Department of Animal Science, University of Agriculture and Environmental
Sciences Umuagwo, Imo State, Nigeria
2Animal Science Department, University of Port Harcourt, Port Harcourt, Nigeria
*Corresponding Author: Agaviezor BO, Animal Science Department, University of Port Harcourt, Port Harcourt, Nigeria.
June 23, 2023; Published: July 13, 2023
The study investigated the polymorphism of myxovirus resistance (Mx) gene in four Nigerian chicken genotypes. A total of 200 chickens comprising of 50 Normal feathered, 50 Frizzle feathered, 50 Naked neck, and 50 Noiler chickens. The chickens were kept for 16 weeks during which feed and water were given ad libitum. Other management practices were carried out. Blood samples were collected from the chickens via the wing vein using 2ml syringe into an EDTA bottle for DNA extraction. Polymerase Chain Reaction (RCR) was carried out using the DNA extracted and primers of Mx gene. PCR amplicons were sequences and sequences subjected to analysis. Results obtained showed the highest genetic distance is between the Frizzled feather and Naked neck (0.1537) and the least is between the Noiler and the Normal feather (0.0755). Nucleotide diversity with JC (PiJC) obtained for Normal feathered, Naked neck, Noiler and Frizzled feather genotype were 0.01005, 0.05868, 0.01973 and 0.17961. Gst value obtained (0.04437) indicated that 95.56% genetic variation within populations and only 4.44% between populations. The estimated Nm value (5.38) suggested genetic differentiation among populations of Nigerian chicken genotypes impeded by high gene flow. This could be further supported by the result obtained in the genetic distance which suggested relatedness among the chicken populations studied.
Keywords: Myxovirus Resistance Gene; Polymorphisms; Noiler; Naked Neck; Normal Feather; Frizzled Feather
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