Muhammad UmerAnwer¹*, Saima Aslam², Muhammad Bilal Anwar³, Asad Manzoor² Misbah Ijaz² and Faizan Saleem¹

¹BahauddinZakariyya University, Multan, Pakistan
²University of Agriculture, Faisalabad, Pakistan
³University of Sargodha, Pakistan

*Corresponding Author: Muhammad UmerAnwer, BahauddinZakariyya University, Multan, Pakistan.

Received: April 25, 2023; Published: July 10, 2023

Abstract

The increasing resistance against antimicrobials has encouraged the alertness in the innovation and preparation of various non-antibiotics antimicrobials. The resistance against different antibiotics in bacteria is a vital process usually happening and crucial apprehension in whole of the ecosphere. The principal collective nosocomial pollutant comprises of Staphylococcus (S.) epidermidiscontamination. It is manifestly obvious from the ancient times of the establishment of pharmaceutical agents, that to some extent, a medicine may have diverse functions and has appreciated belongings in exclusively transformed zones of medication. Resistance of medicines against microorganisms documents analysis of firsthand antibiotics which were operational against resistant microorganisms. Subsequently, the simultaneous investigation was programmed to analyze the antimicrobial belongings of a non-antibiotic antimicrobial medicine, diclofenac sodium againstS. epidermidis isolate of bacteria. The research comprised of two sections i.e., In vitro susceptibility examination and minimum inhibitory concentration (MIC) of diclofenac sodium was assessed beside S. epidermidis during the early section of the investigation and in vivo susceptibility examination by topical application of diclofenac sodium over the surgical wounds. Diclofenac sodium generates no area of growth inhibition at 2, 5 and 10µg/disc concentration against all three isolates while creates zone of growth inhibition computing 16, 18 and 20 mm at concentration of 25, 50 and 100µg/disc against S. epidermidis correspondingly, however for judgment sulphamethoxazole discs were applied as standard, which created a region of growth inhibition computing 22 mm against the similar isolate. The standard medicine formed a region of growth retardation measuring 24 mm. Minimum inhibitory concentration values was measured by agar dilution method was 25µg/ml for S. epidermidis. When diclofenac sodium topically applied to the wounds then the cuff/gr determined was varied from 4.86 ± 0.04 X 106 while 5.68 ± 0.07 x 106colony forming units/gram was determined in normal saline cured wounds.

Keywords: Surgical Wounds; Antibacterial Activity; Diclofenac; Staphylococcus; Invivo; Invitro

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Citation

Citation: Muhammad UmerAnwer., et al. “Activity of Diclofenac Sodium (I) In Vitro Susceptibility of Selected Pathogens and, (II) In Vivo Evaluation against Staphylococcus epidermidis in Surgical Wound Infection Model Antibacterial".Acta Scientific Veterinary Sciences 5.8 (2023): 14-19.

Copyright

Copyright: © 2023 Muhammad UmerAnwer., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.