Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 5 Issue 5

Serum Biochemical Effects of Alstonia boonei Leaf Extract Administered against Coccidiosis in Broilers

Ukar IA1,2*, Ifenkwe DC3, Ezea J2, Herbert U2 and Oguike MA2

1Advanced Space Technology Applications Laboratory, National Space Research and Development Agency, Uyo, Akwa Ibom, State, Nigeria
2Department of Animal Breeding and Physiology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
3College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria

*Corresponding Author: Ukar IA, Advanced Space Technology Applications Laboratory, National Space Research and Development Agency, Uyo, Akwa Ibom, State, Nigeria.

Received: March 23, 2023; Published: April 27, 2023


Thirty-six (36) nulliparous mixed-breed rabbit does, aged, 5-6 months were subjected to a scientific study to determine the effects of methanolic extracts of a medicinal plant, king of bitter (Andrographis paniculata) on the haematology and serum biochemical profile of rabbits does. The rabbits were randomly selected and assigned into four (4) treatment groups designated as T1, T2, T3 and T4 in a completely randomized design Each treatment comprised nine (9) animals. The treatments were replicated 3 times with 3 animals per replicate. 1g of dried methanolic extract of A. paniculata was reconstituted in 100ml of distilled water to form a liquid suspension which was administered orally to the rabbit; early in the morning using a sterile syringe for a period of 8weeks, at the rates of 0.0mg/kg, 125mg/kg, 250mg/kg and 500mg/kg respectively for T1, T2, T3 and T4, with T1 being the control experiment. Blood samples were collected from 6 animals per treatment group (a total of twenty-four rabbits) selected randomly across each replicate, at the onset of the experiment for basal studies. Subsequently, samples were collected fortnightly at week 4, 6, and 8, of administration of the extract for haematological and serum biochemical analysis. Data obtained were subjected to Analysis of variance; significant means were separated using Duncan’s multiple range test. The results obtained at the end of the study showed significant differences (P <0.05) in some of the blood parameters evaluated. RBC count increase significantly (P < 0.05), with increase in administration rate of the A. paniculata extract. There was however a decline in Hb, MCH and MCHC values. PCV, WBC, platelets and Lymphocyte counts were observed to be statistically similar. Serum glucose levels decreased significant with increase in consumption rate of the extract. An increase in Serum albumin, globulin and Total protein was recorded. Higher values were recorded in T2 and T3 for Alanine Transaminase while Aspartate Transaminase values tended to decline. It is concluded from this study that methanolic extracts of A. paniculata had significant influence on blood parameters of domestic rabbit.

Keywords: Extracts; Nulliparous; Rabbits; Does; Treatments; Haematology


  1. “Expert consultation on rural poultry and rabbit production”. Rome. FAO secretariat (1981).
  2. Orayaga KT., et al. “Carcass and fur yield, internal organ characteristics and GIT morphometry of rabbits fed diets containing composite mango (Mangifera indica) reject meal”. MOJ Food processing and Technology 3 (2017): 307-312.
  3. Daramola JO., et al. “Haematological and serum biochemical parameters of West African Dwarf (WAD) goats”. Livestock Research for Rural Development8 (2015).
  4. Andrade-Neto VF., et al. “Antimalarial activity of Cinchona-like plants used to treat fever and malaria in Brazil”. Journal of Ethnopharmacology2-3 (2003): 253-256.
  5. Jarukamjorn K and Nemoto N. “Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituent andrographolide”. Journal of Health Science 54 (2008): 370-381.
  6. Blumenthal M., et al. “Therapeutic Guide to Herbal Medicines. The Complete German Commission E Monographs. Austin”. American Botanical Council and Boston (1998).
  7. Coon JT and Ernst E. “Andrographis Paniculata in the Treatment of Upper Respiratory Tract Infections: A Systematic Review of Safety and Efficacy”. Planta Medica (2004).
  8. Agro meteorological unit, National Root Crop Research Institute, Umudike, Nigeria (2007).
  9. Reitma S and Frankel S. “A Calorimetric Method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase”. American Journal of Clinical Pathology 28 (1953): 5663.
  10. Roschlan P., et al. “Enzymatische best immung de gesant cholesterium in serum”. Journal of Clinical Chemistry and Biochemistry 12 (1974): 403-407.
  11. Steel RGD and Torrie JH. “Principles and procedures of statistics. A biometrical approach”. 2nd McGraw-Hill Publications. New York (1980): 633.
  12. Duncan DB.Multiple range and multiple F tests”. Biometrics 11 (1955): 1-4.
  13. Ojebiyi OO., et al. “Haematology, Carcass and Relative Organ Weights of Growing Rabbits on Skip-A-Day Concentrate Feeding Regime”. International Journal of Applied Agricultural and Apicultural Research 1-2 (2013): 167-174.
  14. Ogbuewu IP. “Physiological responses of rabbits fed graded level of neem (Azadirachta indica) leaf meal. M.Sc Thesis. Federal University of Technology, Owerri”. Imo State (2008).
  15. Chmurska-Gąsowska M., et al. “Haematological changes in rabbits (Oryctolagus cuniculus f. domesticus) in the course of pregnancy”. Animal Reproduction 2 (2021): e20210013.
  16. Research Animal Resource [RAR]. “Reference values for laboratory animals: Normal haematological values. RAR Websites, University of Minnesota (2009).
  17. “Mean cell haemoglobin” (2013f).
  18. Husen R., et al. “Screening for antihyperglycemic activity in several local herbs of Malaysia”. Journal of Ethnopharmacology 95 (2004): 205-208.
  19. Dandu AM and Inamdar NM. “Evaluation of beneficial effects of antioxidant properties of aqueous leaf extract of Andrographis paniculata in STZ-induced diabetes”. Pakistan Journal of Pharmaceutical Science 22 (2009): 49-52.
  20. Shahid A. “Andrographis paniculata: A Review of Pharmacological Activities and Clinical Effect”. Alternative Medicine Review1 (2011): 66.


Citation: Ukar IA., et al. “Haematology and Serum Biochemistry of Rabbit does Administered Methanolic Extracts of Andrographis paniculata". Acta Scientific Veterinary Sciences 5.5 (2023): 53-58.


Copyright: © 2023 Ukar IA., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US