Parvathy AP¹*, K Murugan², Vilash V¹ and Lakshmi AP¹

¹Department of Botany, Sree Narayana College, Kollam, Kerala, India
²CISSA, Thiruvananthapuram, Kerala, India

*Corresponding Author: Parvathy AP, Sree Narayana College, Kollam, Kerala, India.

Received: March 21, 2023; Published: April 10, 2023

Abstract

Herbals play an immense role in the primary health needs of local communities in developing countries. Nowadays, there is renewed interest in medicinal plants that have emerged because of their availability and less or nontoxic nature. The therapeutic potential of leaf and stem extracts of Tinospora crispa were analyzed by FTIR and UV-Vis spectroscopy. Major active molecules in the stem and leaf extract were interpreted using their functional groups. The peak (cm-1), intensity, corresponding intensity, base (H), base (L), area and corresponding area obtained from FTIR spectra data confirmed the presence of phenol, alkanes, aldehyde, secondary alcohol, amino acid, aromatic amines and halogens. The IR peak at 2800 to 2900 cm-1 refers to the presence of alkanes (C-H stretch), peak at 1700 to 1600 cm-1 corresponds the carboxylic acid group (C = O stretch), peak at 1500 cm-1 denotes the 1 ° amines (N-H bend), peak of 1300 to 1200 cm-1 showed the presence of aromatic amines (C-N stretch), peaks of 1200 to 1100 cm-1 indicate the alcohols, carboxylic acids, esters, ethers (C-O stretch). A peak of 600 -800 cm-1 revealed the alkenes (=C-H bend). Prominent UV-visible spectra peaks at 222, 350, 361, 317 nm, and 408 nm with the corresponding absorption reveal the presents of polyphenolic and alkaloids. Thus, the results of the present study provide a platform for using T. crispa leaves and stem as herbal alternative having multiple therapeutic potentials like anti-inflammatory, antioxidant, immunomodulatory, cytotoxic, antimalarial, cardioprotective, and anti-diabetic potentialities.

Keywords: Tinospora crispa; Secondary Metabolites; Medicinal Plants; FTIR Spectrum; Diseases

References

1. Ahmad Waqas., et al. “Tinospora crispa (L.) Hook. f. and Thomson: A Review of Its Ethnobotanical, Phytochemical, and Pharmacological Aspects”. Frontiers in Pharmacology 7 (2016).
2. Agatonovic-Kustrin Snezana., et al. “The Use of Fourier Transform Infrared (FTIR) Spectroscopy and Artificial Neural Networks (ANNs) to Assess Wine Quality”. Modern Chemistry and Applications4 (2013).
3. Rahman, Mariam Abdul., et al. “Phytochemical and Biological Activity Studies of Tinospora crispa Stem”. The Dhaka University Journal of Science2 (2020): 167-170.
4. Kumar J and Devi Prasad. “Identification and Comparison of Biomolecules in Medicinal Plants of Tephrosia tinctoria and Atylosia albicans by Using FTIR Romanian”. Journal Biophysical 1 (2011): 63-71.
5. Apriandanu D and Yulizar Y. “The Role of Aqueous Leaf Extract of Tinospora crispa as Reducing and Capping Agents for Synthesis of Gold Nanoparticles”. IOP Conf. Series: Materials Science and Engineering, International Symposium on Current Progress in Functional Materials (2017).
6. Rajiv P., et al. “Screening for Phytochemicals and FTIR Analysis of Myristica dactyloids Fruit Extracts”. International Journal of Pharmacy and Pharmaceutical Science 9 (2016): 315-318.
7. Muruganantham, S., et al. “FT-IR and SEMEDS Comparative Analysis of Medicinal Plants, Eclipta alba Hassk and Eclipta prostrata Linn”. Romanian Journal of Biophysics 19 (2009): 285-294.
8. Sahithya S and Krishnaveni. “Ft-Ir and GC-MS Analysis of Stem Extract of Ethnomedicinal Plant: Bridelia montana (Roxb)”. Analysis of Stem Extract of Ethnomedicinal Plant: Bridelia montana (Roxb.) Asian Journal of Biological and Life Sciences2 (2022): 451-456.
9. Njokua DI., et al. “Corrosion inhibition of mild steel in hydrochloric acid solution by the leaf extract of Nicotiana tabacum”. Advanced Materials: Corrosion 1 (2013): 54-61.
10. Karpagasundari C and S Kulothungan. “Analysis of Bioactive Compounds in Physalis minima Leaves Using GC MS, HPLC, UV-VIS and FTIR Techniques”. Journal of Pharmacognosy and Phytochemistry 4 (2014): 196-201.

Citation

Citation: Parvathy AP., et al. “Comparison of UV-Visible Spectrophotometric and FTIR Analysis of Tinospora Crispa (l.) Hook. F. and Thomson Leaves and Stem". Acta Scientific Veterinary Sciences 5.5 (2023): 12-16.

Copyright

Copyright: © 2023 Parvathy AP., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.