Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 5 Issue 3

Epitope tailored vaccine construct for goat milk allergy

Nidhi Sukhija1#, Kanaka K K1#*, Sona Sukhija2, Nandhini P B1, Pallavi Rathi1, Vidhyasagar3, Indrajit Ganguly4, SP Dixit4, Sanjeev Singh4 and Chethan Raj5

1ICAR-National Dairy Research Institute, Karnal, Haryana, India
2Government Medical College, Haldwani, Uttarakhand, India
3Veterinary College Bidar, KVAFSU, Karnataka, India
4ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
5ICAR-Indian Veterinary Research Institute, Izatnagar, UP, India
#First two authors contributed equally to the paper

*Corresponding Author:Kanaka K K, ICAR-National Dairy Research Institute, Karnal, Haryana, India.

Received: January 30, 2023; Published: February 10, 2023


Goat milk is less allergic because concentration of milk proteins is lower than that of cattle milk. But we can’t rule out the possibility of allergies from goat milk. This study is conducted to check whether goat milk is allergic and antigenic epitopes are found. Six major milk proteins were downloaded from public domain. CTL, HTL and B cell epitopes were detected and 1 to 5 epitopes from each type in each study proteins crossed threshold, hence proving goat milk would also be allergic. Further topmost epitope from each category and linkers were used to develop vaccine against goat milk allergy. The designed vaccine was of the length 371 amino acid residues and was demonstrated to be strong antigenic while being non-allergic and non-toxic. Molecular docking of the epitopes was done against TLR3 and TLR4 and found to be very well interacting with the epitopes which was indicated in negative binding energies. Further immuno simulations were done and found that the designed vaccine was able to stimulate production of immune molecules

Keywords: Goat; Milk allergy; Epitope; Vaccine; Immunoinformatic


  1. Conesa C., et al. “Isolation of lactoferrin from milk of different species: calorimetric and antimicrobial studies”. Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 1 (2008): 131-139.
  2. McCullough FS. “Nutritional evaluation of goat’s milk”. British Food Journal (2003).
  3. Di Sabatino A and Corazza GR. “Epitope-specific immunotherapy: A vaccine for coeliac disease?” The Lancet Gastroenterology and Hepatology7 (2017): 464-465.
  4. Ehlayel M., et al. “Camel milk: An alternative for cow’s milk allergy in children”. Allergy and Asthma Proceedings: The Official Journal of Regional and State Allergy Societies 32 (2011): 255-258.
  5. Gasteiger E., et al. “ExPASy: The proteomics server for in‑depth protein knowledge and analysis”. Nucleic Acids Research 31 (2003): 3784‑3788.
  6. Doytchinova IA and Flower DR. “VaxiJen: A server for prediction of protective antigens, tumour antigens and subunit vaccines”. BMC Bioinformatics 8 (2007): 1‑7.
  7. Dimitrov I., et al. “AllerTOP‑a server for in silico prediction of allergens. In BMC Bioinformatics”. Bio Med Central 14 (2013): 1‑9.
  8. Chauhan V., et al. “Designing a multi‑epitope-based vaccine to combat Kaposi Sarcoma utilizing immunoinformatics approach”. Scientific Reports 9 (2019): 1‑15.
  9. Geourjon C and Deleage G. “SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments”. Bioinformatics 11.6 (1995): 681-684.
  10. Laskowski R., et al. “PROCHECK: validation of protein-structure coordinates” (2006).
  11. Wiederstein M and Sippl MJ. “ProSA-web: Interactive web service for the recognition of errors in three-dimensional structures of proteins”. Nucleic Acids Research 35.2 (2007): W407-W410.
  12. Kozakov D., et al. “The ClusPro web server for protein-protein docking”. Nature Protocols 12.2 (2017): 255-278.
  13. Rapin N., et al. “Computational immunology meets bioinformatics: The use of prediction tools for molecular binding in the simulation of the immune system”. PloS One 4 (2010): e9862.
  14. Van Regenmortel MH. “Mapping epitope structure and activity: From one-dimensional prediction to four-dimensional description of antigenic specificity”. Methods 3 (1996): 465-472.
  15. Hoft DF., et al. “Optimizing vaccine development”. Cellular Microbiology 13.7 (2011): 934-942.
  16. Perrie Y., et al. “Vaccine adjuvant systems: Enhancing the efficacy of sub-unit protein antigens”. International Journal of Pharmaceutics 364.2 (2008): 272-280.
  17. Ojha R., et al. “Strategic development of a next-generation multi-epitope vaccine to prevent Nipah virus zoonotic infection”. ACS Omega 4.8 (2019): 13069-13079.
  18. Pandey RK., et al. “Exploring dual inhibitory role of febrifugine analogues against Plasmodium utilizing structure-based virtual screening and molecular dynamic simulation”. Journal of Biomolecular Structure and Dynamics 4 (2017): 791-804.
  19. Nezafat N., et al. “Designing an efficient multi-epitope peptide vaccine against Vibrio cholerae via combined immunoinformatics and protein interaction based approaches”. Computational Biology and Chemistry 62 (2016): 82-95.
  20. Bergmann CC., et al. “Flanking residues alter antigenicity and immunogenicity of multi-unit CTL epitopes”. The Journal of Immunology 8 (1996): 3242-3249.
  21. Shahid F., et al. “Immunoinformatics guided rational design of a next generation multi epitope based peptide (MEBP) vaccine by exploring Zika virus proteome”. Infection, Genetics and Evolution 80 (2020): 104199.
  22. Aranda V., et al. “Shared apical sorting of anion exchanger isoforms AE2a, AE2b1, and AE2b2 in primary hepatocytes”. Biochemical and Biophysical Research Communications 319.3 (2004): 1040-1046.
  23. Patil VM., et al. “Chapter 14 - Dengue Virus Polymerase: A Crucial Target for Antiviral Drug Discovery. In S. P. Gupta (Ed.), Viral Polymerases (2019): 387-428. Academic Press.
  24. Kalita P., et al. “Design of a peptide-based subunit vaccine against novel coronavirus SARS-CoV-2”. Microbial Pathogenesis 145 (2020): 104236.
  25. Flicker S., et al. “Passive immunization with allergen-specific antibodies”. Vaccines against Allergies (2011): 141-159.
  26. Srinivasan S., et al. “Epitope identification and designing a potent multi-epitope vaccine construct against SARS-CoV-2 including the emerging variants”. Journal of Global Infectious Diseases 1 (2022): 24.


Citation: Nidhi Sukhija., et al. “Epitope tailored vaccine construct for goat milk allergy". Acta Scientific Veterinary Sciences 5.3 (2023): 23-30.


Copyright: © 2023 Nidhi Sukhija., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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