Epitope tailored vaccine construct for goat milk allergy
Nidhi Sukhija1#, Kanaka K K1#*, Sona Sukhija2, Nandhini P B1, Pallavi Rathi1, Vidhyasagar3, Indrajit Ganguly4, SP Dixit4, Sanjeev Singh4 and Chethan Raj5
1ICAR-National Dairy Research Institute, Karnal, Haryana, India
2Government Medical College, Haldwani, Uttarakhand, India
3Veterinary College Bidar, KVAFSU, Karnataka, India
4ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana, India
5ICAR-Indian Veterinary Research Institute, Izatnagar, UP, India
#First two authors contributed equally to the paper
*Corresponding Author:Kanaka K K, ICAR-National Dairy Research Institute, Karnal, Haryana, India.
January 30, 2023; Published: February 10, 2023
Goat milk is less allergic because concentration of milk proteins is lower than that of cattle milk. But we can’t rule out the possibility of allergies from goat milk. This study is conducted to check whether goat milk is allergic and antigenic epitopes are found. Six major milk proteins were downloaded from public domain. CTL, HTL and B cell epitopes were detected and 1 to 5 epitopes from each type in each study proteins crossed threshold, hence proving goat milk would also be allergic. Further topmost epitope from each category and linkers were used to develop vaccine against goat milk allergy. The designed vaccine was of the length 371 amino acid residues and was demonstrated to be strong antigenic while being non-allergic and non-toxic. Molecular docking of the epitopes was done against TLR3 and TLR4 and found to be very well interacting with the epitopes which was indicated in negative binding energies. Further immuno simulations were done and found that the designed vaccine was able to stimulate production of immune molecules
Keywords: Goat; Milk allergy; Epitope; Vaccine; Immunoinformatic
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