Harinivas Manthira Moorthi, Allan Amalan Jeffrin P and Phibi Philip Naduvathu*

Institute of Fisheries Biotechnology, Tamil Nadu Dr. J. Jayalalithaa Fisheries University OMR campus, Chennai, India

*Corresponding Author: Phibi Philip Naduvathu, Institute of Fisheries Biotechnology, Tamil Nadu Dr. J. Jayalalithaa Fisheries University OMR campus, Chennai, India.

Received: December 28, 2022; Published: January 27, 2023

Abstract

Aquaculture is rapidly replacing wild fish with farmed fish as the principal source of seafood in human diets. Diseases, reduced viability, fertility decline, poor growth, farmed fish escape into wild, and environmental pollution are the major issues faced by the sector. The commercialization of AquaAdvantage salmon and CRISPR/Cas9-developed tilapia (Oreochromis niloticus) initiated using of genetic engineering and genome editing methods, such as CRISPR/Cas, as potential solutions to overcome the challenges. Disease resistance, sterility, and improved growth are among the future features being developed in many fish species. CRISPR/Cas is less expensive, simpler, and more precise than existing genome editing technologies, and it can be employed as a new breeding method in fisheries and aquaculture to address major difficulties. Furthermore, unlike transgenesis, which introduces foreign genes into the host genome and thus alleviates major public safety concerns, CRISPR/Cas genome editing rapidly introduces favourable changes by disrupting genes with targeted minor changes. Although CRISPR/Cas technology has enormous potential, there are a number of technical hurdles as well as regulatory and public issues involving its usage in fisheries and aquaculture. Nonetheless, the exciting point in the CRISPR/Cas9 genome editing is that two CRISPR-edited fish, namely, red sea bream and tiger puffer developed by the Kyoto-based startup, have received approval and are now available for purchase, while another fish, FLT-01 Nile tilapia developed by AquaBounty, is not classified as a genetically modified organism regulatory. However, it is still a long way from revolutionizing and becoming a viable commercial aquaculture breeding technology for aquaculture-important features and species.

Keywords: CRISPR/Cas; Aquaculture; Gene Editing; Growth; Transgenesis

References

1. Ceasar S Antony., et al. "Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9”. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research9 (2016): 2333-2344.
2. Chakrapani Vemulawada., et al. "Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9”. Developmental and Comparative Immunology61 (2016): 242-247.
3. Chen Ji., et al. "Efficient gene transfer and gene editing in sterlet (Acipenser ruthenus)”. Frontiers in Genetics9 (2018): 117.
4. Cleveland Beth M., et al. "Editing the duplicated insulin-like growth factor binding protein-2b gene in rainbow trout (Oncorhynchus mykiss)”. Scientific Reports1 (2018): 1-13.
5. Dan Cheng., et al. "A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)”. Science Bulletin21 (2018): 1420-1430.
6. Datsomor Alex K., et al. "CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes”. Scientific Reports9.1 (2019): 1-13.
7. Datsomor Alex K., et al. "CRISPR/Cas9-mediated editing of Δ5 and Δ6 desaturases impairs Δ8-desaturation and docosahexaenoic acid synthesis in Atlantic salmon (Salmo salar L.)”. Scientific Reports1 (2019): 1-13.
8. Doudna Jennifer A and Emmanuelle Charpentier. "The new frontier of genome engineering with CRISPR-Cas9”. Science6213 (2014): 1258096.
9. Edvardsen Rolf B., et al. "Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation”. PloS One9 (2014): e108622.
10. The State of World Fsheries and Aquaculture 2020: Sustainability in Action (Rome: FAO) (2020).
11. Gan Rui-Hai., et al. "Functional divergence of multiple duplicated foxl2 homeologs and alleles in a recurrent polyploid fish”. Molecular Biology and Evolution5 (2021): 1995-2013.
12. Güralp Hilal., et al. "Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon”. Scientific Reports1 (2020): 1-12.
13. Hille Frank and Emmanuelle Charpentier. "CRISPR-Cas: biology, mechanisms and relevance”. Philosophical Transactions of the Royal Society B: Biological Sciences1707 (2016): 20150496.
14. https://www.takarabio.com/learning-centers/gene-function/gene-editing/gene-editing-tools-and-information/introduction-to-the-crispr/cas9-system
15. Ibrahim, A., et al. "Genome engineering using the CRISPR/Cas9 system”. Journal of Biomedical and Pharmaceutical Sciences 2 (2019).
16. Jiang Dongneng., et al. "CRISPR/Cas9-induced disruption of wt1a and wt1b reveals their different roles in kidney and gonad development in Nile tilapia”. Developmental Biology1 (2017): 63-73.
17. Jiang Fuguo and Jennifer A Doudna. "CRISPR-Cas9 structures and mechanisms”. Annual Review of Biophysics 1 (2017): 505-529.
18. Jinek Martin., et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity”. Science6096 (2012): 816-821.
19. Khalil Karim., et al. "Generation of myostatin gene-edited channel catfish (Ictalurus punctatus) via zygote injection of CRISPR/Cas9 system”. Scientific Reports1 (2017): 1-12.
20. Kim Julan., et al. "CRISPR/Cas9-mediated myostatin disruption enhances muscle mass in the olive flounder Paralichthys olivaceus”. Aquaculture512 (2019): 734336.
21. Kishimoto, Kenta., et al. "Production of a breed of red sea bream Pagrus major with an increase of skeletal muscle mass and reduced body length by genome editing with CRISPR/Cas9”. Aquaculture495 (2018): 415-427.
22. Kleppe Lene., et al. "Sex steroid production associated with puberty is absent in germ cell-free salmon”. Scientific Reports1 (2017): 1-11.
23. Li Minghui., et al. "Efficient and heritable gene targeting in tilapia by CRISPR/Cas9”. Genetics2 (2014): 591-599.
24. Li Qiuhua., et al. "Effective CRISPR/Cas9-based genome editing in large yellow croaker (Larimichthys crocea)”. Aquaculture and Fisheries1 (2023): 26-32.
25. Liu, et al. "Methodologies for improving HDR efficiency”. Frontiers in Genetics9 (2019): 691.
26. Liu Qingfeng., et al. "Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny”. Science China Life Sciences9 (2019): 1194-1202.
27. Liu Zhenquan., et al. "Application of different types of CRISPR/Cas-based systems in bacteria”. Microbial Cell Factories1 (2020): 1-14.
28. Mei Yue., et al. "Recent progress in CRISPR/Cas9 technology”. Journal of Genetics and Genomics2 (2016): 63-75.
29. Ming S H A O., et al. "The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models”. Zoological Research4 (2016): 191.
30. Porteus Matthew. "Genome editing: a new approach to human therapeutics”. Annual Review of Pharmacology and Toxicology56 (2016): 163-190.
31. Rath Devashish., et al. "The CRISPR-Cas immune system: biology, mechanisms and applications”. Biochimie117 (2015): 119-128.
32. Segev-Hadar Adi., et al. "Genome Editing Using the CRISPR-Cas9 System to Generate a Solid-Red Germline of Nile Tilapia (Oreochromis niloticus)”. The CRISPR Journal4 (2021): 583-594.
33. Shimbun Y. “Kyoto Frm Puts Genome-Edited Tiger Pufer on the Table (Japan: Japan News)” (2021).
34. Straume Anne Hege., et al. "Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors”. BMC Genomics1 (2021): 1-8.
35. Sun Yuan., et al. "Disruption of mstna and mstnb gene through CRISPR/Cas9 leads to elevated muscle mass in blunt snout bream (Megalobrama amblycephala)”. Aquaculture528 (2020): 735597.
36. Sun Yuying., et al. "CRISPR/Cas9-mediated deletion of one carotenoid isomerooxygenase gene (EcNinaB-X1) from Exopalaemon carinicauda”. Fish and Shellfish Immunology97 (2020): 421-431.
37. Tao Binbin., et al. "CRISPR/Cas9 system-based myostatin-targeted disruption promotes somatic growth and adipogenesis in loach, Misgurnus anguillicaudatus”. Aquaculture544 (2021): 737097.
38. Xie Qing-Ping., et al. "Haploinsufficiency of SF-1 causes female to male sex reversal in Nile tilapia, Oreochromis niloticus”. Endocrinology6 (2016): 2500-2514.
39. Xu Xiuwen., et al. "Production of a mutant of large-scale loach Paramisgurnus dabryanus with skin pigmentation loss by genome editing with CRISPR/Cas9 system”. Transgenic Research3 (2019): 341-356.
40. Yamano, et al. "Crystal structure of Cpf1 in complex with guide RNA and target DNA”. Cell165.4 (2016): 949-962.
41. Yang, et al. "Methods favoring homology-directed repair choice in response to CRISPR/Cas9 induced-double strand breaks”. International Journal of Molecular Sciences21.18 (2020): 6461.
42. Yu Hong., et al. "Targeted gene disruption in Pacific oyster based on CRISPR/Cas9 ribonucleoprotein complexes”. Marine Biotechnology3 (2019): 301-309.
43. Zhai Gang., et al. "Successful production of an all-female common carp (Cyprinus carpio) population using cyp17a1-deficient neomale carp”. Engineering8 (2022): 181-189.
44. Zhong Zhaomin., et al. "Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp”. Scientific Reports1 (2016): 1-14.
45. Zu Yao., et al. "Biallelic editing of a lamprey genome using the CRISPR/Cas9 system”. Scientific Reports1 (2016): 1-9.
46.  

Citation

Citation: Phibi Philip Naduvathu., et al. “CRISPR Genome Editing Technology to Revolutionize Aquaculture and Fisheries".Acta Scientific Veterinary Sciences 5.2 (2023): 63-70.

Copyright

Copyright: © 2023 Phibi Philip Naduvathu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.