Kartik Samanta², Seema Rani Pegu², Gyanendra Singh Sengar², Kartik Samanta¹, Aditya Pratap Acharya¹ and Hemanta Kumar Maity¹

¹Department of Avian Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India
²Department of Animal Health, ICAR-National Research Centre on Pig, Guwahati, Assam, India

*Corresponding Author: Hemanta Kumar Maity, Department of Avian Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India.

Received: December 16, 2022; Published: January 12, 2023

Abstract

The porcine circovirus 2 (PCV2) is one of the most economically significant swine pathogens, causing PCVAD as well as impaired reproductive efficiency, stillbirth, and weight loss in infected pig populations. The PCV2 ORF2 gene encodes capsid protein (cap protein), which has 233 amino acids (AA) in PCV2a/2b and an additional lysine residue at the C-terminus in PCV2d. In this study, we created a PCV2d capsid sequence (ORF2) for expression in Escherichia coli. His tag chromatography method was used to purify the protein, and western blot was used to detect protein expression. In this study, an indirect enzyme-linked immunosorbent assay (I-ELISA) based on the PCV2d ORF2 protein was developed. The best antigen, serum, and goat anti-porcine IgG conjugate dilutions were 1:1500 (3.245 μg/ml), 1:320, and 1:1600, respectively. The estimated cut-off value was 0.9885, and its analytical sensitivity was 1:2400. Except for PCV2a, PCV2b, and PCV2d antisera, the results of specific evaluation revealed that these antisera did not cross-react with any other common porcine viral pathogens, indicating that this method can be used to detect porcine circovirus infection in pig.

Keywords: PCV2d; ORF2; Capsid protein; Expression; E coli

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Citation

Citation: Hemanta Kumar Maity.,et al. “Expression of PCV2d Capsid Protein in Escherichia coli and its Evaluation for Serodiagnosis of Porcine Circovirus Infection".Acta Scientific Veterinary Sciences 5.2 (2023): 28-33.

Copyright

Copyright: © 2023 Hemanta Kumar Maity.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.