Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 5 Issue 1

Characterization of Microsatellite Markers in Crossbred Ducks

Purabi Kaushik1*, Raj Jyoti Deka1, Dishanta Dutta2, Indu Borah Dutta2, Mrinal Bora2, BN Bhattacharya1 and DC Mili1

1College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
2Government Duck and Poultry Farm, Joysagar, Sivasagar, Assam, India

*Corresponding Author: Purabi Kaushik, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.

Received: July 11, 2022; Published: Decemeber 08, 2022

Abstract

The study was conducted at College of Veterinary Science, Assam Agricultural University, Khanapara, Assam. In this study, the genomic DNA was isolated and characterized microsatellite loci in crossbred duck (Pati x White Pekin). Ten microsatellites were used to detect polymorphisms in 50 cross bred ducks. A total of 28 nos of alleles were observed and all loci were polymorphic. Per microsatellite locus number of alleles ranged from 2 to 5 with an average of 2.6 ±0.08. The observed and expected heterozygosity of these polymorphic makers ranged from 0.00 to 0.42 with an average number of 0.047 and 0.41 to 0.82 with an average number of 0.57. The Polymorphic markers consist of observed heterozygosity’s of loci were less than 0.50. The polymorphism information content (PIC) of 10 loci ranged from 0.32 to 0.78 with an average of 0.477. Microsatellite markers can be a tool for mitigating the genetic linkage map of the duck as well as comparative mapping with the chicken.

Keywords: Microsatellite Markers; Polymorphism; PIC; Cross Bred (Pati duck White Pekin)

References

  1. Alyethodi Rafeeque and Kumar Sanjeev. “Genetic Characterization of Moti Indian Native Duck Using Microsatellite Markers”. Journal of Applied Animal Research1 (2010): 223.
  2. Botstein D., et al. “Construction of a genetic linkage map in man using restriction fragment length polymorphisms”. The American Journal of Human Genetics 32 (1980): 314-331.
  3. Hamilton MB., et al. “Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites”. Biotechniques 27 (1999): 500-507.
  4. Hsu YC., et al. “Isolation and characterization of microsatellite DNA markers from the Lanyu scops owl (Otus elegans botelensis)”. Molecular Ecology Notes 3 (2003): 595-597.
  5. Huang YH., et al. “Characterization of 35 novel microsatellite DNA markers from the duck (Anas platyrhynchos) genome and cross-amplification in other birds”. Genetics Selection Evolution 37 (2005): 455-472.
  6. Huang YH., et al. “A Genetic and Cytogenetic Map for the Duck (Anas platyrhynchos)”. Genetics 173 (2006): 287-296.
  7. Maak S., et al. “Isolation and characterization of 18 microsatellites in the Peking duck (Anas platyrhynchos) and their application in other waterfowl species”. Molecular Ecology Notes 3 (2003): 224-227.
  8. Yeh FC., et al. “POPGENE Version 1.32: Microsoft Window-Based Freeware for Population Genetics Analysis”. University of Alberta, Edmonton (1999).

Citation

Citation: Purabi Kaushik., et al. “Characterization of Microsatellite Markers in Crossbred Ducks".Acta Scientific Veterinary Sciences 5.1 (2023): 12-16

Copyright

Copyright: © 2022 Purabi Kaushik., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is June 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US