Purabi Kaushik¹*, Raj Jyoti Deka¹, Dishanta Dutta², Indu Borah Dutta², Mrinal Bora², BN Bhattacharya¹ and DC Mili¹

¹College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
²Government Duck and Poultry Farm, Joysagar, Sivasagar, Assam, India

*Corresponding Author: Purabi Kaushik, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India.

Received: July 11, 2022; Published: Decemeber 08, 2022

Abstract

The study was conducted at College of Veterinary Science, Assam Agricultural University, Khanapara, Assam. In this study, the genomic DNA was isolated and characterized microsatellite loci in crossbred duck (Pati x White Pekin). Ten microsatellites were used to detect polymorphisms in 50 cross bred ducks. A total of 28 nos of alleles were observed and all loci were polymorphic. Per microsatellite locus number of alleles ranged from 2 to 5 with an average of 2.6 ±0.08. The observed and expected heterozygosity of these polymorphic makers ranged from 0.00 to 0.42 with an average number of 0.047 and 0.41 to 0.82 with an average number of 0.57. The Polymorphic markers consist of observed heterozygosity’s of loci were less than 0.50. The polymorphism information content (PIC) of 10 loci ranged from 0.32 to 0.78 with an average of 0.477. Microsatellite markers can be a tool for mitigating the genetic linkage map of the duck as well as comparative mapping with the chicken.

Keywords: Microsatellite Markers; Polymorphism; PIC; Cross Bred (Pati duck White Pekin)

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Citation

Citation: Purabi Kaushik., et al. “Characterization of Microsatellite Markers in Crossbred Ducks".Acta Scientific Veterinary Sciences 5.1 (2023): 12-16

Copyright

Copyright: © 2022 Purabi Kaushik., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.