Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 12

GC-MS Analysis and Wound Healing Potential of Ficus racemosa L. Gum in Wistar Albino Rats

Madhuribahen Ratishkumar Patel1*, Akash Golaviya2, Anjali Bhatia3, Anshuk Sharma4 and Dinesh Kumar5

1Ph.D research scholar, Division of Pharmacology &Toxicology, ICAR-IVRI, Izatnagar, India
2Ph.D research scholar, Department of Microbiology, Kamdhenu University, Anand, Gujarat
3MVSc research scholar, Division of Pharmacology &Toxicology, ICAR-IVRI, Izatnagar, India
4Scientist, Division of Pharmacology & Toxicology, ICAR-IVRI, UP, India
5Principal Scientist, Division of Pharmacology & Toxicology, ICAR-IVRI, Izatnagar, UP, India

*Corresponding Author: Madhuribahen Ratishkumar Patel, Department of Division of Pharmacology, ICAR- Indian Veterinary Research Institute, Izatnagar, India.

Received: November 24, 2022; Published: November 30, 2022

Abstract

Ficus racemosa is Ficus species plant which is popularly known as Country Fig, Udumbara and Gular. Traditionally it is use in various ailments such as ulcers, wound healing, diarrhea, stomachache and skin diseases. Skin wounds represent a major healthcare problem owing to an increasing number of trauma and pathophysiological conditions. Wound healing is a complex, well-orchestrated and regulated process consisting of series of events. Currently, available treatments are limited due to side effects and cost effectiveness. In line with that, we attempted to explore a natural source to study its potential towards the wound healing process. To the best of our knowledge, till date no documented information and published literature available reporting phytochemical profile using GC-MS (Gas chromatography and mass spectrometry) technique as well as animal studies related to healing properties of gum of F. racemosa plant. This work is the first report about the GC-MS analysis and healing potential of F. racemosa gum (FRG). GC-MS analysis of fine powder of FRG was performed by standard protocol using Perkin Elmer-Autosystem XL GC with TurboMass software which is, interfaced to MS Perkin Elmer TurboMass. GC-MS analysis identified three phytoconstituents such as, pentadecanoic acid (7.59%), hexadecyne (90.38%) and 1-undecene 9-methyl (2.04%). Then, its potential in wound healing on surgically wounded wistar rats was assessed by conducting two trials of 14 days and 24 days. During trials the wounds were visually observed, photographically documented and the wound area was measured. Results revealed that groups treated with F. racemosa gum (3%FRG and 1%FRG) showed considerable and progressive reduction in wound area (mm2) as well as significant percentage wound contraction and faster complete closure of wounds compare to control group. This study suggested that the gum of F. racemosa might be a potential therapeutic agent for skin wound healing, supporting its traditional medicinal uses.

Keywords: Ficus Racemosa; Gum; GS-MS; Phytochemical; Wound Healing

References

  1. Pahari N., et al. “Exploring the Pharmacognostic properties and pharmacological activities of phytocompounds present in Ficusracemosalinn.: A concise review”. Pharmacological Research-Modern Chinese Medicine (2022): 100137.
  2. Kosankar KV and Aher AN. “The Phytoconstituents and Pharmacological Actions of Ficusracemosa Linn (Family: Moraceae)-An updated review”. PharmaTutor12 (2018): 55-63.
  3. Paarakh MP. “Ficus racemosa Linn. An overview”. Indian Journal of Natural Products and Resources 1 (2009): 84-90.
  4. Deep P., et al. “Pharmacological potentials of Ficus racemosa-a review”. International Journal of Pharmaceutical Sciences Review and Research1 (2013): 29-34.
  5. Yadav RK., et al. “Phytochemistry, pharmacology, toxicology, and clinical trial of Ficus racemosa”Pharmacognosy Reviews 17 (2015): 73.
  6. Ahmed F and Urooj A. “Traditional uses, medicinal properties, and phytopharmacology of Ficus racemosa: A review”. Pharmaceutical Biology6 (2010): 672-681.
  7. Sumi SA., et al. “Investigation of the key pharmacological activities of Ficus racemosa and analysis of its major bioactive polyphenols by HPLC-DAD”. Evidence Based Complementary and Alternative Medicine (2016).
  8. Murti K and Kumar U. “Enhancement of wound healing with roots of Ficus racemosa in albino rats”. Asian Pacific Journal Tropical Biomedicine 2.4 (2012): 276-280.
  9. Pingale, T., et al. “Antibacterial and antifungal approaches of Ficus racemosa”Pharmacognosy magazine11 (2019): 2.
  10. Bhalerao SA., et al. “Ficus racemosa: a comprehensive review”. Journal of Applied Chemistry 3.4 (2014): 1423-1431.
  11. , et al. “Wound healing: a cellular perspective”. Physiological Reviews 99.1 (2019): 665-706.
  12. Darwin E and Tomic-Canic M. “Healing chronic wounds: current challenges and potential solutions”. Current Dermatology Report4 (2018): 296-302.
  13. Gottrup F. “A specialized wound-healing center concept: importance of a multidisciplinary department structure and surgical treatment facilities in the treatment of chronic wounds”. American Journal of Surgery5 (2004): 38-43.
  14. , et al. “Chronic wounds”. Nature Reviews Disease Primers 8.1 (2022): 1-21.
  15. , et al. “Effects of 3 topical plant extracts on wound healing in beef cattle”. African Journal of Traditional, Complementary and Alternative Medicine 9.4 (2012): 542-547.
  16. https://www.google.com/url?sa=i&url=https%3A%2F%2Fwww.plantsguru.com%2Fgoolarplant&psig=AOvVaw3W4eCzfOSHd4Vms1EKzqab&ust=1669482117996000&source=images&c =vfe&ved=0CBAQjRxqFwoTCIinkdfnyfsCFQAAAAAdAAAAABAE
  17. https://www.google.com/imgres?imgurl=https%3A%2F%2Fthumbs.dreamstime.com%2Fb%2Fficus-racemosa-aththikka-cluster-fig-tree-cluster-fig-red-river-fig-gular-species-familymoraceae-ficus-
  18. Mamani R and Alhaji NM. “GC-MS analysis of phytocomponents in methanolic extract of Coleus aromaticus”. Journal of Pharmacognosy and Phytochemistry4 (2019): 106-109.
  19. Preethi R., et al. "Antimicrobial and antioxidant efficacy of some medicinal plants against food borne pathogens”. Advances in biological Research 2 (2010): 122-125.
  20. El-Beltagi., et al. "GC-MS analysis, antioxidant, antimicrobial and anticancer activities of extracts from Ficus sycomorus fruits and leaves". Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2 (2019): 493-505.
  21. , et al. "Icariin improves cutaneous wound healing in streptozotocin-induced diabetic rats”. Journal of Tissue Viability 31.1 (2022): 197-206.
  22. Sharma A., et al. "Topical application of paraffin-based Curcuma longa L. rhizome powder ointment diminished post-surgical algesia and promoted wound closure”. Indian Journal of Veterinary Surgery1 (2022): 1-5.
  23. , et al. “Angiogenic and MMPs modulatory effects of icariin improved cutaneous wound healing in rats". European Journal of Pharmacology 858 (2019): 172466.
  24. Khalid MN., et al. "The bioactivity and phycochemistry of two stonewort algae (Charophycota) from Sindh”. Proceedings of the Pakistan Academy of Science 4 (2010): 205-214.
  25. Basker and Anusha. "Phytochemical analysis and GC-MS profiling in the leaves of Sauropusandrogynus (l) MERR”. International Journal of Drug Development and Research1 (2012).
  26. , et al. "A review on the phytochemistry, medicinal properties and pharmacological activities of 15 selected Myanmar medicinal plants”. Molecules 24.2 (2019): 293.
  27. , et al. "Linum usitatissimum as an antimicrobial agent and a potential natural healer: A review”. Archives of Orofacial Science 13.2 (2018).
  28. , et al. "The physiology of wound healing”. Surgery (Oxford) 35.9 (2017): 473-477.
  29. Han George and Roger Ceilley. "Chronic wound healing: a review of current management and treatments”. Advances in Therapy3 (2017): 599-610.
  30. , et al. "Wound healing: Cellular mechanisms and pathological outcomes”. Open biology. 10.9 (2020): 200223.
  31. Lavanya A., et al. “Evaluation of Ficus racemosa leaf for wound healing activity”. International Journal of Pharmaceutics 12 (2016): 501-512.
  32. Gupta R and Gupta J. “Ointment of methanolic extract of Ficus religiosa: A traditional approach in wound healing in rats”. International Journal of Pharmaceutical Sciences Research 12 (2016): 5006.
  33. Garg VK and Paliwal SK. “Wound-healing activity of ethanolic and aqueous extracts of Ficus benghalensis”. Journal of Advanced Pharmaceutical Technology and Research2 (2011): 110.

Citation

Citation: Madhuribahen Ratishkumar Patel., et al. “GC-MS Analysis and Wound Healing Potential of Ficus racemosa L. Gum in Wistar Albino Rats".Acta Scientific Veterinary Sciences 4.12 (2022): 203-210

Copyright

Copyright: © 2022 Madhuribahen Ratishkumar Patel., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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