GC-MS Analysis and Wound Healing Potential of Ficus racemosa L. Gum in Wistar Albino Rats
Madhuribahen Ratishkumar Patel1*, Akash Golaviya2, Anjali Bhatia3,
Anshuk Sharma4 and Dinesh Kumar5
1Ph.D research scholar, Division of Pharmacology &Toxicology, ICAR-IVRI, Izatnagar, India
2Ph.D research scholar, Department of Microbiology, Kamdhenu University, Anand, Gujarat
3MVSc research scholar, Division of Pharmacology &Toxicology, ICAR-IVRI, Izatnagar, India
4Scientist, Division of Pharmacology & Toxicology, ICAR-IVRI, UP, India
5Principal Scientist, Division of Pharmacology & Toxicology, ICAR-IVRI, Izatnagar, UP, India
*Corresponding Author: Madhuribahen Ratishkumar Patel, Department of Division of Pharmacology, ICAR- Indian Veterinary Research Institute, Izatnagar, India.
November 24, 2022; Published: November 30, 2022
Ficus racemosa is Ficus species plant which is popularly known as Country Fig, Udumbara and Gular. Traditionally it is use in various ailments such as ulcers, wound healing, diarrhea, stomachache and skin diseases. Skin wounds represent a major healthcare problem owing to an increasing number of trauma and pathophysiological conditions. Wound healing is a complex, well-orchestrated and regulated process consisting of series of events. Currently, available treatments are limited due to side effects and cost effectiveness. In line with that, we attempted to explore a natural source to study its potential towards the wound healing process. To the best of our knowledge, till date no documented information and published literature available reporting phytochemical profile using GC-MS (Gas chromatography and mass spectrometry) technique as well as animal studies related to healing properties of gum of F. racemosa plant. This work is the first report about the GC-MS analysis and healing potential of F. racemosa gum (FRG). GC-MS analysis of fine powder of FRG was performed by standard protocol using Perkin Elmer-Autosystem XL GC with TurboMass software which is, interfaced to MS Perkin Elmer TurboMass. GC-MS analysis identified three phytoconstituents such as, pentadecanoic acid (7.59%), hexadecyne (90.38%) and 1-undecene 9-methyl (2.04%). Then, its potential in wound healing on surgically wounded wistar rats was assessed by conducting two trials of 14 days and 24 days. During trials the wounds were visually observed, photographically documented and the wound area was measured. Results revealed that groups treated with F. racemosa gum (3%FRG and 1%FRG) showed considerable and progressive reduction in wound area (mm2) as well as significant percentage wound contraction and faster complete closure of wounds compare to control group. This study suggested that the gum of F. racemosa might be a potential therapeutic agent for skin wound healing, supporting its traditional medicinal uses.
Keywords: Ficus Racemosa; Gum; GS-MS; Phytochemical; Wound Healing
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