Madhuribahen Ratishkumar Patel*, Abdul Saddam, Akash Golaviya, W Ramdas Singh, Anshuk Sharma, Sanjay Kumawat and Dinesh Kumar

Department of Division of Pharmacology, ICAR- Indian Veterinary Research Institute, Izatnagar, India

*Corresponding Author: Madhuribahen Ratishkumar Patel, Department of Division of Pharmacology, ICAR- Indian Veterinary Research Institute, Izatnagar, India.

Received: November 18, 2022; Published: November 29, 2022

Abstract

Wound healing is an integral part of reestablishment of damaged structure. Many studies have reported that turmeric/Curcuma longa L. (C. longa) has significant anti-inflammatory, antioxidant, anti-infective and wound healing properties. Curcumin, is a one of the main active constituent of C. longa which acts on various stages of the natural wound healing process to hasten healing. In a previous study from our laboratory, it was found out that individual fractions or components and pure phytochemicals are sometimes less bioactive than crude, multicomponent extracts, i.e., most of the bioactive constituents possessing synergistic activity are lost during fractionation, isolation or processing. So, in the present study, we have compared the wound healing potential of C. longa preparations such as curcumin, C. longa alcoholic extract (CLAE) and crude C. longa rhizome powder (CLRP) in cutaneous wound model in normal rats by creating an open excision-type wound. The ointments were applied topically on the wound area twice daily for 14 days. The wound photography and contraction measurement were done on days 0, 3, 7, 11 and 14 post-wounding. Portion of granulation tissues were harvested, after euthanizing rats on 14th day, for assessment of pro-healing parameters level, oxidative parameters level and histopathological changes. The gross wound assessment revealed that among all groups, CLRP-treated groups showed greater wound contraction and faster wound closure which was followed by CLAE-treated group, curcumin and control groups. The levels of pro-healing and anti-oxidative parameters were high in treatment groups compared to control group. In histopathological study, CLRP-treated groups showed good thickness of epithelialization, marked fibroblast proliferation, angiogenesis, enhanced collagen deposition and low level of polymorph nuclear lymphocytes (PMNL) in granulation tissue compared to other groups. All treatment groups have shown faster wound healing property when compared to control group. Moreover, CLRP showed higher healing potential compared to curcumin and C. CLAE-treated groups. Therefore, findings of the present study revealed that C. longa have efficient antioxidant activities, which might have accelerated the wound healing process by preventing lipid peroxidation mediated damage to the biological structures, and by scavenging free radicals. Our findings further suggest that pure curcumin might not be the only active pharmacological moiety present in C. longa for exhibiting wound healing property.

Keywords: Curcuma longa, Curcumin, cutaneous wound model, wound healing

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Citation

Citation: Madhuribahen Ratishkumar Patel., et al. “Comparison of Wound Healing Potential of Curcumin, Alcoholic Extract and Powder of Curcuma longa L. in Rats".Acta Scientific Veterinary Sciences 4.12 (2022): 195-202.

Copyright

Copyright: © 2022 Madhuribahen Ratishkumar Patel., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.