Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 12

Documentation of Seminal Plasma Protein Profiles in Dromedary Camels

Gorakh Mal1,2*, Sumant Vyas1, Birbal Singh2 and Nitin V Patil1

1National Research Centre on Camel, Bikaner, Rajasthan, India
2Indian Veterinary Research Institute, Regional Station, Palampur, H.P, India

*Corresponding Author: Gorakh Mal, National Research Centre on Camel, Bikaner, Rajasthan, India.

Received: October 03, 2022; Published: November 16, 2022

Abstract

The purpose of this study was to establish the protein profile of seminal plasma of dromedary camels with reference to their rutting behavior. Semen ejaculates were obtained from seven adult male dromedary Jaisalmeri camels. Five camels were performing copulation successfully and two camels not showing all the signs of rutting. Fresh semen samples were centrifuged at 6000 rpm for 30 min. and clear supernatant of seminal plasma was separated and stored at -20oC until used. Total protein from the seminal plasma was estimated by Lowry Method and the molecular weight was analysed by SDS-PAGE. A total of 10 protein bands were observed in the seminal plasma of rutting camels ranged from 11.7-104.0 kDa, whereas, 9 protein bands were observed in the seminal plasma of non-rutting camels. These 9 protein bands were found to be common in rutting and non-rutting camels. However, additional protein band of 40.0 kDa with very strong intensity was observed only in rutting camels and found to be heat resistant at 38 ºC up to 18 hours. The relevance of 40.0 kDa proteins in the fertility status of dromedary camels deserves further investigation.

Keywords: Dromedary Camel; Fertility Marker; Seminal Plasma Protein; SDS-PAGE Analysis

References

  1. Kulkarni BA. “Current status of research on seminal plasma proteins”. Indian Journal of Animal Reproduction 24 (2003): 1-
  2. El-Naggar M and Abdel-Raouf M. “The electrophoretic pattern and the amino acid content of the seminal plasma protein”. Indian Veterinary Journal 54 (1977): 239-
  3. Ali HA., et al. “On the morphology of the accessory male glands and histochemistry of the ampulla ductus deferent is of the camel (Camelus dromedarius)”. Journal of Anatomy 125 (1978): 277-
  4. Chen BX., et al. “Factors inducing ovulation in the Bactrian camel”. In: Cockrill WR, editor. “The Camelid an All-Purpose Animal”. Scandinavian Institute of African Studies. Uppsala, Sweden 1 (1984): 387-
  5. , et al. “Ovulation-inducing factor: a protein component of llama seminal plasma”. Reproductive Biology and Endocrinology 8 (2010): 44-50.
  6. Ratto MH., et al. “The nerve of ovulation-inducing factor in semen”. The Proceedings of the National Academy of Sciences 109 (2012): 15042-15047.
  7. Kershaw-Young CM., et al. “β-Nerve growth factor is a major component of alpaca seminal plasma and induces ovulation in female alpacas”. Reproduction Fertility Development 24 (2012): 1093-
  8. Druart X., et al. “Proteomic characterization and cross species comparison of mammalian seminal plasma”. Journal of Proteomics 91 (2013): 13-22.
  9. Hafez ESE.“Reproduction in Farm Animals”. 6th Lea and Febiger, Philadelphia (1993).
  10. Adams GP., et al. “Ovulation-inducing factor in the seminal plasma of alpacas and llamas”. Biology of Reproduction 73 (2005): 452-457.
  11. Vyas S., et al. “Use of Tris and lactose extenders in preservation of camel semen at refrigerated temperature”. Indian Veterinary Journal 75 (1998): 810-
  12. Mal G., et al. “Protein and mineral profiles in the seminal plasma of dromedary camels”. Indian Veterinary Journal 88 (2011): 82-
  13. Lowry OH., et al. “Protein measurement with the folin phenol reagent”. Journal of Biological Chemistry 193 (1951): 265-275.
  14. Laemmli UK. “Cleavage of structural proteins during the assembly of the head of Bacteriophage T4”. Nature 227 (1970): 680-685.
  15. Killian G., et al. “Fertility associated proteins in Holstein bull seminal plasma”. Biology of Reproduction 49 (1993): 1202-1207.
  16. Brandon CI., et al. “Two-dimensional polyacrylamide gel electrophoresis of equine seminal plasma proteins and their correlation with fertility”. Theriogenology 53 (1999): 863-873.
  17. Billah M and Skidmore JA. “The collection, evaluation and deep-freezing of dromedary camel semen”. In: Proceedings of the First International Camel Conference, Dubai, UAE (1992): 61.
  18. Deen A and Sahani MS. “Preliminary attempts to collect and cryopreserve camel semen”. Journal of Camel Practice and Research 7 (2000): 181-186.
  19. Singh J., et al. “Seminal plasma electrolytes in the dromedary camel and their correlation with seminal quality”. Indian Journal of Animal Science 71 (2001): 782-
  20. Singh S., et al. “Mating behaviour of one humped camel in relation to body condition score”. Ruminant Science 5 (2016): 235-237.
  21. Deen A., et al. “Semen collection, cryopreservation and artificial insemination in the dromedary camel”. Animal Reproduction Science 77 (2003): 223-233.
  22. Bravo PW., et al. “Reproductive aspects and storage of semen in camelidae”. Animal Reproduction Science 62 (2000): 173-193.
  23. Garnica J., et al. “Physical and biochemical characteristics of alpaca semen”. Animal Reproduction Science 32 (1993): 85-90.
  24. Zeidan AEB and Abbas HE. “Physiological and biochemical changes in the male dromedary camels during rutting and nonbreeding seasons”. Journal of Camel Practice and Research 11 (2003): 183-190.
  25. Mattner PE. “The survival of spermatozoa in bovine cervical mucus and mucus fractions”. Journal of Reproductive Fertility 20 (1969): 193-
  26. Padalino B., et al. “Male camel behavior and breeding management strategies: How to handle a camel bull during the breeding season?” Emiratus Journal of Food Agriculture 27 (2015): 338-
  27. Singer R., et al. “Protein content of human seminal plasma and spermatozoa in relation to sperm counts”. Acta Europaea Fertilities 7 (1976): 281-284.
  28. Qu F., et al. “The role of Zn-alpha2 glycoprotein in sperm motility is mediated by changes in cyclic AMP”. Reproduction 134 (2007): 569-
  29. Kumar S., et al. “Proteomic identification of camel seminal plasma: Purification of β-nerve growth factor”. Animal Reproduction Science 136 (2013): 289-295.
  30. Mal G., et al. “Studies on liquefaction time and proteins involved in the Improvement of seminal characteristics in dromedary camels (Camelus dromedarius)". Scientifica (2016).
  31. Mal G., et al. “Comparative study of seminal plasma proteins in dromedary camels”. Indian Journal of Animal Science 84 (2014): 13-14.

Citation

Citation: Gorakh Mal., et al. “Documentation of Seminal Plasma Protein Profiles in Dromedary Camels".Acta Scientific Veterinary Sciences 4.12 (2022): 70-75.

Copyright

Copyright: © 2022 Gorakh Mal., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US