Documentation of Seminal Plasma Protein Profiles in Dromedary Camels
Gorakh Mal1,2*, Sumant Vyas1, Birbal Singh2 and Nitin V Patil1
1National Research Centre on Camel, Bikaner, Rajasthan, India
2Indian Veterinary Research Institute, Regional Station, Palampur, H.P, India
*Corresponding Author: Gorakh Mal, National Research Centre on Camel, Bikaner, Rajasthan, India.
October 03, 2022; Published: November 16, 2022
The purpose of this study was to establish the protein profile of seminal plasma of dromedary camels with reference to their rutting behavior. Semen ejaculates were obtained from seven adult male dromedary Jaisalmeri camels. Five camels were performing copulation successfully and two camels not showing all the signs of rutting. Fresh semen samples were centrifuged at 6000 rpm for 30 min. and clear supernatant of seminal plasma was separated and stored at -20oC until used. Total protein from the seminal plasma was estimated by Lowry Method and the molecular weight was analysed by SDS-PAGE. A total of 10 protein bands were observed in the seminal plasma of rutting camels ranged from 11.7-104.0 kDa, whereas, 9 protein bands were observed in the seminal plasma of non-rutting camels. These 9 protein bands were found to be common in rutting and non-rutting camels. However, additional protein band of 40.0 kDa with very strong intensity was observed only in rutting camels and found to be heat resistant at 38 ºC up to 18 hours. The relevance of 40.0 kDa proteins in the fertility status of dromedary camels deserves further investigation.
Keywords: Dromedary Camel; Fertility Marker; Seminal Plasma Protein; SDS-PAGE Analysis
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