Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 11

Effects of A1A1 and A2A2 Casein in Cow's Milk on Mice Intestine, Heart, and Spleen

Shikha Yadav1, Bilal Ur Rehman3, Mohammad Ahmad Khan2 and Vijay Pal Singh3

1National Institute of Biologicals, Noida, India 2Department of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India 3CSIR-Institute of Genomics and Integrative Biology, New Delhi, India

*Corresponding Author:Vijay Pal Singh, CSIR-Institute of Genomics and Integrative Biology, New Delhi, India.

Received: October 04, 2022; Published: October 18, 2022


Milk is considered to be a complete dietary food that is consumed all over the world as a protein source. It has various proteins among which A1 and A2 variants of Beta-casein are the major varieties. Amidst them, A1 is believed to possess health issues. One of its metabolite Beta-CasoMorphin-7 (BCM7) is becoming a research point of interest among the scientific community. As the intestine is the site where maximum absorption of milk takes place, exploration of this intestinal tissue is a must. Along with it we also observed Heart and Spleen tissues for the effects of milk but there was no pathological change found in these organs. Histopathology is the key technique that has been used in our study to demonstrate the influence of A1 and A2 milk on the intestine. The goal of our study was to establish any possible relationship between the intestinal health of BALB/c mice and the consumption of milk containing A1A1 beta casein variants while no pathological changes were found in mice fed with the A2A2 variants of Beta casein. To our understanding, this is the opening study showing increased intestinal accessibility and villi atrophy in all the mice given with A1A1 milk which in turn may lead to nutrient deficiency and further health issues in these mice. In contrast, no pathological changes were found in the control mice and in those that were administered with the A2A2 Beta casein variant of cow milk.

Keywords: A1A1 and A2A2; Beta-Casomorphine7 (BCM7); Villous Atrophy; Intestinal Permeability


  1. Sabikhi L. “Designer milk”. Advances in Food and Nutrition Research 53 (2007): 161-198.
  2. Yadav S., et al. “Oral Feeding of Cow Milk Containing A1 Variant of β Casein Induces Pulmonary Inflammation in Male Balb/c Mice”. Scientific Reports1 (2020): 1-8.
  3. Yadav S., et al. “A1 β-Casein variant of cow milk exacerbates House Dust Mite induced Allergic airway disease in a murine model”. IJBPAS1 (2021): 326-345.
  4. Ul Haq MR., et al. “Comparative evaluation of cow beta-casein variants (A1/A2) consumption on Th2-mediated inflammatory response in the mouse gut”. European Journal of Nutrition 4 (2014): 1039-1049.
  5. “Ministry of Fisheries, Animal Husbandry and Dairying”. Census Cattle Buffalo 5 (2022): 57.
  6. Behera R., et al. “A1 versus A2 Milk”. International Journal of Livestock Research 4 (2018): 1-7.
  7. Woodford K. “A1 Beta-casein, Type 1 Diabetes and Links to other Modern Illnesses”. Diabetes Research and Clinical Practice 79 (2018): 1-16.
  8. Guantario B., et al. “A Comprehensive Evaluation of the Impact of Bovine Milk Containing Different Beta-Casein Profiles on Gut Health of Ageing Mice”. Nutrients7 (2020): 2-19.
  9. Murphy LY Wan., et al. “Influence of functional food components on gut health”. Critical Reviews in Food Science and Nutrition 12 (2019): 1927-1936.

10.   Suzuki T. “Regulation of intestinal epithelial permeability by tight junctions”. Cellular and Molecular Life Sciences 70.4 (2013): 631-659.


Citation: Vijay Pal Singh., et al. “Effects of A1A1 and A2A2 Casein in Cow's Milk on Mice Intestine, Heart, and Spleen". Acta Scientific Veterinary Sciences 4.11 (2022): 117-120.


Copyright: © 2022 Noura Mohammed Almaimouni., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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