Greeshma AJ¹*, Ramani Pushpa RN², Lakshmi Kavitha K³ and Srinivasa Rao T⁴

¹Post Graduate, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India ²Professor, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India ³Associate Professor, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India ⁴Associate Professor, Department of Veterinary Public Health, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India

*Corresponding Author:Greeshma AJ, Post Graduate, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India.

Received: October 10, 2022; Published: October 18, 2022

Abstract

It is very important to study and understand the antimicrobial resistance pattern of these infectious agents in order to provide best treatment regime. The present study is on antimicrobial resistance pattern of S. aureus and S. uberis causing mastitis. Disc diffusion test of S. aureus isolates showed that 69.35% of isolates were resistant to Penicillin G, followed by Ampicillin [50%], Erythromycin [30.64%], Methicillin [29.03%], Clindamycin [24.19%], Amoxycillin/Clavulanic acid [20.96%], Streptomycin [20.96%] and least resistance was shown to Gentamicin, Cotrimoxazole, Chloramphenicol, Tetracycline and Vancomycin. MRSA and MSSA has shown a great variation in antibiotic resistance pattern, MRSA was 2 to 4 times more resistant to all tested antibiotics. MRSA showed a high resistance to Penicillin G, Ampicillin, Clindamycin and Streptomycin. All MRSA isolates were biofilm producers which may be favouring to the increased antimicrobial resistance. All isolates were carrying blaZ gene and all except one isolate was carrying mecA gene. In vitro antibiotic sensitivity test of S. uberis revealed that 59% of the isolates were resistant to Ceftriaxone followed by Streptomycin, Erythromycin, Penicillin G, Tetracycline, Amoxycillin clavulanic acid, Enrofloxacin and most of the isolates were sensitive to Gentamicin, Chloramphenicol and Ampicillin/Sulbactam. Non biofilm forming S. uberis isolates were sensitive to Tetracycline, Chloramphenicol, Ampicillin/Sulbactam, Amoxycillin/Clavulanic acid, Gentamicin and Enrofloxacin whereas biofilm formers were resistant to these antibiotics by 30.76%, 30.76%, 2.56%, 25.64%, 15.38% and 25.64% respectively.

Keywords: Antimicrobial Resistance; S, aureus; S, uberis; Antibiotic Sensitivity Test; Methicillin Resistant S, aureus; Biofilm and Antimicrobial Resistance

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Citation

Citation: Greeshma AJ., et al. “Antimicrobial Resistance Pattern of Staphylococcus aureus and Streptococcus uberis Causing Mastitis". Acta Scientific Veterinary Sciences 4.11 (2022): 107-116.

Copyright

Copyright: © 2022 Noura Mohammed Almaimouni., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.