Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 11

Antimicrobial Resistance Pattern of Staphylococcus aureus and Streptococcus uberis Causing Mastitis

Greeshma AJ1*, Ramani Pushpa RN2, Lakshmi Kavitha K3 and Srinivasa Rao T4

1Post Graduate, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India 2Professor, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India 3Associate Professor, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India 4Associate Professor, Department of Veterinary Public Health, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India

*Corresponding Author:Greeshma AJ, Post Graduate, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India.

Received: October 10, 2022; Published: October 18, 2022

Abstract

It is very important to study and understand the antimicrobial resistance pattern of these infectious agents in order to provide best treatment regime. The present study is on antimicrobial resistance pattern of S. aureus and S. uberis causing mastitis. Disc diffusion test of S. aureus isolates showed that 69.35% of isolates were resistant to Penicillin G, followed by Ampicillin [50%], Erythromycin [30.64%], Methicillin [29.03%], Clindamycin [24.19%], Amoxycillin/Clavulanic acid [20.96%], Streptomycin [20.96%] and least resistance was shown to Gentamicin, Cotrimoxazole, Chloramphenicol, Tetracycline and Vancomycin. MRSA and MSSA has shown a great variation in antibiotic resistance pattern, MRSA was 2 to 4 times more resistant to all tested antibiotics. MRSA showed a high resistance to Penicillin G, Ampicillin, Clindamycin and Streptomycin. All MRSA isolates were biofilm producers which may be favouring to the increased antimicrobial resistance. All isolates were carrying blaZ gene and all except one isolate was carrying mecA gene. In vitro antibiotic sensitivity test of S. uberis revealed that 59% of the isolates were resistant to Ceftriaxone followed by Streptomycin, Erythromycin, Penicillin G, Tetracycline, Amoxycillin clavulanic acid, Enrofloxacin and most of the isolates were sensitive to Gentamicin, Chloramphenicol and Ampicillin/Sulbactam. Non biofilm forming S. uberis isolates were sensitive to Tetracycline, Chloramphenicol, Ampicillin/Sulbactam, Amoxycillin/Clavulanic acid, Gentamicin and Enrofloxacin whereas biofilm formers were resistant to these antibiotics by 30.76%, 30.76%, 2.56%, 25.64%, 15.38% and 25.64% respectively.

Keywords: Antimicrobial Resistance; S, aureus; S, uberis; Antibiotic Sensitivity Test; Methicillin Resistant S, aureus; Biofilm and Antimicrobial Resistance

References

  1. Riffon Renee., et al. "Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR”. Journal of Clinical Microbiology 7 (2001): 2584-2589.
  2. Zecconi A and Piccinini R. “Intramammary infections: epidemiology and diagnosis”. XXII World Buiatrics congress 18-23 Hannover, Germany 8 (2002): 346-359
  3. Bhosale Rohit Rajendra., et al. "Mastitis: an intensive crisis in veterinary science”. International Journal of Pharma Research and Health Sciences 2 (2014): 96-103.
  4. Srivastava AK. "Recent concepts on the use of antimicrobials in the treatment of mastitis”. Verma, R. and Mamtani, AP (eds.) (2000): 62-83.
  5. Barkema H W., et al. "Invited review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis”. Journal of Dairy Science6 (2006): 1877-1895.
  6. Silveira-Filho Vladimir M., et al. "Antibiotic resistance and molecular analysis of Staphylococcus aureus isolated from cow's milk and dairy products in northeast Brazil”. Journal of Food Protection4 (2014): 583-591.
  7. Li Xian-Zhi., et al. "β-Lactam resistance and β-lactamases in bacteria of animal origin”. Veterinary Microbiology3-4 (2007): 197-214.
  8. Young M P and Thomas C M. e-Therapeutics PLC. Antibacterial combination therapy for the treatment of gram positive bacterial infections. U.S. Patent 8 (2013) 614,179.
  9. Singh Shriti., et al. "Understanding the mechanism of bacterial biofilms resistance to antimicrobial agents”. The Open Microbiology Journal 11 (2017): 53.
  10. Melchior MB., et al. "Biofilms: a role in recurrent mastitis infections?”. The Veterinary Journal3 (2006): 398-407.
  11. Vakkamaki Johanna., et al. "Bacteriological etiology and treatment of mastitis in Finnish dairy herds”. Acta Veterinaria Scandinavica 1 (2017): 1-9.
  12. Oliver Stephen P., et al. "Impact of antibiotic use in adult dairy cows on antimicrobial resistance of veterinary and human pathogens: a comprehensive review”. Foodborne Pathogens and Disease3 (2011): 337-355.
  13. Beyene Takele., et al. "Prevalence and antimicrobial resistance profile of Staphylococcus in dairy farms, abattoir and humans in Addis Ababa, Ethiopia”. BMC Research Notes 1 (2017): 1-9.
  14. Oliveira L and P L Ruegg. "Treatments of clinical mastitis occurring in cows on 51 large dairy herds in Wisconsin”. Journal of Dairy Science 9 (2014): 5426-5436.
  15. Pascu Corina., et al. "Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania”. Antibiotics1 (2022): 57.
  16. National Mastitis Council Inc. “Microbiological Procedures for the Diagnosis of Bovine Udder Infection”. 3rd Arlington, Virginia: National Mastitis Council, (1990).
  17. Sudhan N A., et al. "Studies on prevalence, etiology and diagnosis of subclinical mastitis among crossbred cows”. Indian Journal of Animal Research2 (2005): 127-130.
  18. Phillips Ian. "Medical Microbiology. Volume two: the Practice of Medical Microbiology: Edited by R. Cruickshank, JP Duguid, BP Marmion and RHA Swain”. 1975. Edinburgh, London and New York: Churchill Livingstone.
  19. Anand Kumar P. "Evaluation of PCR test for detecting major pathogens of bubaline mastitis directly from mastitic milk samples of buffaloes”. Tropical Animal Health and Production8 (2009): 1643-1651.
  20. Dhanawade Nirmala B., et al. "Detection of intercellular adhesion genes and biofilm production in Staphylococcus aureus isolated from bovine subclinical mastitis”. Veterinary Research Communications1 (2010): 81-89.
  21. Christensen Gordon D., et al. "Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices”. Journal of Clinical Microbiology6 (1985): 996-1006.
  22. Merritt Judith H., et al. "Growing and analyzing static biofilms”. Current Protocols in Microbiology1 (2011): 1B-1.
  23. Moore Glenis Elizabeth. "Biofilm Production by Streptococcus uberis Associated with Intramammary Infections”. (2009).
  24. Wayne PA. "Clinical and laboratory standards institute; 2007”. Performance standards for antimicrobial susceptibility testing. CLSI document M100-S17 (2005).
  25. Mohanty Nihar., et al. "Isolation and antibiogram of Staphylococcus, Streptococcus and Escherichia coli isolates from clinical and subclinical cases of bovine mastitis”. Veterinary World10 (2013).
  26. Bradley Andrew J. "Bovine mastitis: an evolving disease”. The Veterinary Journal2 (2002): 116-128.
  27. Tuteja FC. “Studies on mastitis in buffaloes with reference to serum selenium status and control by treating teat canal infections”. Diss. Ph. D. Thesis, CCS HAU, Hisar, Haryana, India, (1999).
  28. Davies PL., et al. "Molecular epidemiology of Streptococcus uberis clinical mastitis in dairy herds: strain heterogeneity and transmission”. Journal of Clinical Microbiology1 (2016): 68-74.
  29. Petzer Inge-Marie., et al. "Intramammary infection rate during the dry period in cows that received blanket dry cow therapy: efficacy of 6 different dry-cow intra-mammary antimicrobial products”. Journal of the South African Veterinary Association1 (2009): 23-30.
  30. Ebrahimi A and Taheri M Akhavan. "Characteristics of staphylococci isolated from clinical and subclinical mastitis cows in Shahrekord, Iran”. (2009): 273-277.
  31. H Muhamed., et al. "Antimicrobial drug susceptibility of Staphylococcus aureus from subclinical bovine mastitis in Coimbatore, Tamilnadu, South India”. Veterinary World6 (2012): 352.
  32. Ramani RN., et al. "Bacterial etiology and antibiogram of clinical mastitis”. Indian Veterinary Journal2 (2010): 187-188.
  33. Kaliwal Basappa B., et al. "Prevalence and antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis”. Veterinary World4 (2011): 158.
  34. Moon JS., et al. "Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea”. Journal of Dairy Science3 (2007): 1176-1185.
  35. Kaplan Jeffrey B., et al. "Low levels of β-lactam antibiotics induce extracellular DNA release and biofilm formation in Staphylococcus aureus”. Molecular Biology 4 (2012): e00198-12.
  36. Jyothi J Shiva., et al. "Antagonistic effect of ursolic acid on Staphylococcal biofilms”. Veterinary world10 (2018): 1440.
  37. Al-Rubaye., et al. "Biofilm production by Staphylococcus aureus isolated from bovine mastitis related with resistance to the antibiotics”. International Journal of Current Microbiology and Applied Sciences 5 (2016): 33-44.
  38. Elango A., et al. "Bacteriology of sub clinical mastitis and antibiogram of isolates recovered from cross bred cows”. Indian Journal of Animal Research4 (2010): 280-284.
  39. Jain Beenu., et al. "Antibiotic resistance and virulence genes in Streptococcus agalactiae isolated from cases of bovine subclinical mastitis”. Veterinarski arhiv5 (2012): 423-432.
  40. Zhang Tingrui., et al. "Antimicrobial Resistance Profiles and Genes in Streptococcus uberis Associated with Bovine Mastitis in Thailand”. Frontiers in Veterinary Science 8 (2021).
  41. Ghigo Jean-Marc. "Natural conjugative plasmids induce bacterial biofilm development”. Nature 6845 (2001): 442-445.
  42. Donlan Rodney M and J William Costerton. "Biofilms: survival mechanisms of clinically relevant microorganisms”. Clinical Microbiology Reviews2 (2002): 167-193.
  43. Lewis Richard A and Keith GH Dyke. "MecI represses synthesis from the β-lactamase operon of Staphylococcus aureus”. Journal of Antimicrobial Chemotherapy 2 (2000): 139-144.
  44. Berger-Bächi Brigitte and Susanne Rohrer. "Factors influencing methicillin resistance in staphylococci”. Archives of Microbiology3 (2002): 165-171.
  45. Felten Annie., et al. "Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test”. Journal of Clinical Microbiology 8 (2002): 2766-2771.
  46. Prere M F., et al. "Genotype MRSA, a new genetic test for the rapid identification of staphylococci and detection of mecA gene”. Pathologie Biologie 8-9 (2006): 502-505.
  47. Asfour H A E and Somah F Darwish. "Phenotypic and genotypic detection of both mecA-and blaZ-genes mediated β-lactam resistance in Staphylococcus strains isolated from bovine mastitis”. Global Veterinaria1 (2011): 39-50.

Citation

Citation: Greeshma AJ., et al. “Antimicrobial Resistance Pattern of Staphylococcus aureus and Streptococcus uberis Causing Mastitis". Acta Scientific Veterinary Sciences 4.11 (2022): 107-116.

Copyright

Copyright: © 2022 Noura Mohammed Almaimouni., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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