Antimicrobial Resistance Pattern of Staphylococcus aureus and Streptococcus uberis Causing Mastitis
Greeshma AJ1*, Ramani Pushpa RN2, Lakshmi Kavitha K3 and Srinivasa Rao T4
1Post Graduate, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India
2Professor, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India
3Associate Professor, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India
4Associate Professor, Department of Veterinary Public Health, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India
*Corresponding Author:Greeshma AJ, Post Graduate, Department of Veterinary Microbiology, NTR College of Veterinary Sciences, Gannavaram, Andhra Pradesh, India.
October 10, 2022; Published: October 18, 2022
It is very important to study and understand the antimicrobial resistance pattern of these infectious agents in order to provide best treatment regime. The present study is on antimicrobial resistance pattern of S. aureus and S. uberis causing mastitis. Disc diffusion test of S. aureus isolates showed that 69.35% of isolates were resistant to Penicillin G, followed by Ampicillin [50%], Erythromycin [30.64%], Methicillin [29.03%], Clindamycin [24.19%], Amoxycillin/Clavulanic acid [20.96%], Streptomycin [20.96%] and least resistance was shown to Gentamicin, Cotrimoxazole, Chloramphenicol, Tetracycline and Vancomycin. MRSA and MSSA has shown a great variation in antibiotic resistance pattern, MRSA was 2 to 4 times more resistant to all tested antibiotics. MRSA showed a high resistance to Penicillin G, Ampicillin, Clindamycin and Streptomycin. All MRSA isolates were biofilm producers which may be favouring to the increased antimicrobial resistance. All isolates were carrying blaZ gene and all except one isolate was carrying mecA gene. In vitro antibiotic sensitivity test of S. uberis revealed that 59% of the isolates were resistant to Ceftriaxone followed by Streptomycin, Erythromycin, Penicillin G, Tetracycline, Amoxycillin clavulanic acid, Enrofloxacin and most of the isolates were sensitive to Gentamicin, Chloramphenicol and Ampicillin/Sulbactam. Non biofilm forming S. uberis isolates were sensitive to Tetracycline, Chloramphenicol, Ampicillin/Sulbactam, Amoxycillin/Clavulanic acid, Gentamicin and Enrofloxacin whereas biofilm formers were resistant to these antibiotics by 30.76%, 30.76%, 2.56%, 25.64%, 15.38% and 25.64% respectively.
Keywords: Antimicrobial Resistance; S, aureus; S, uberis; Antibiotic Sensitivity Test; Methicillin Resistant S, aureus; Biofilm and Antimicrobial Resistance
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