Immunomodulatory Efficacy of Hedychium Spicatum Rhizome Powder in Long Term Indoxacarb Intoxicated White Leghorn (WLH) Cockerels
Govind Kumar Choudhary1,2, Satya Pal Singh1, Manoj Singh1, Anil Kumar1 and Rajeev Ranjan3*
1G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
2ICAR- IIVR, Krishi Vigyan Kendra, Bhadohi, Uttar Pradesh, India
3ICAR-Directorate of Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand, India
*Corresponding Author: Rajeev Ranjan, ICAR-Directorate of Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand, India. Email Address:(Rajeev.Ranjan1@icar.gov.in)
August 08, 2022; Published: August 30, 2022
The present investigation was undertaken to ascertain the immunomodulatory efficacy of Hedychium spicatum rhizome powder (HSRP) in chronic toxicity produced by indoxacarb in white leghorn (WLH) cockerels by determining the levels of serum immunoglobin (IgY), Interferon-γ (INF-γ), Interleukin -2 (IL-2), and lymphocyte stimulation test (LST), Macrophage function test (MFT) and delayed type hypersensitivity (DTH). 49 white leghorn (WLH), 6-8 weeks old chicks were divided into 07 groups consisting of 7 birds in each gr. Group I was maintained at grower ration whereas groups II to VII were given medicated ration upto 16 weeks. The immunomodulant activity of HSRP was determined using both Cell mediated immune response (CMI) i.e., LST, MFT and DTH and Humoral mediated immune response (HMI) i.e., by evaluating antibody titre against bovine serum albumin (BSA) used as antigen. Quantification of chicken IL-2 and INF-γ were carried out by ELISA method as per the manufacture instructions using standard curve method. The result of present finding indicates that the indoxacarb producing the immunosuppression in long term intoxication in WLH cockerels and simultaneous feeding of rhizome powder of H spicatum (HSRP) @ 2000ppm and 4000ppm produced immunomodulatory effects in dose dependent manner by elevation of IgY, INF-γ and IL-2 titres. The decreases in these parameters by indoxacarb reveal the immunosuppression and simultaneous treatment with HSRP improves these values indicating the immunomodulatory properties of H spicatum and can be use Ayurvedic medicines alone.
Key words: Hedychium Spicatum; Immunomodulation; Interleukin-2; Interferon-Y; Indoxacarb; White Leghorn Cockerel
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