Govind Kumar Choudhary^1,2, Satya Pal Singh¹, Manoj Singh¹, Anil Kumar¹ and Rajeev Ranjan³*

¹G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
²ICAR- IIVR, Krishi Vigyan Kendra, Bhadohi, Uttar Pradesh, India
³ICAR-Directorate of Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand, India

*Corresponding Author: Rajeev Ranjan, ICAR-Directorate of Foot and Mouth Disease, Mukteshwar, Nainital, Uttarakhand, India. Email Address:(Rajeev.Ranjan1@icar.gov.in)

Received: August 08, 2022; Published: August 30, 2022

Abstract

The present investigation was undertaken to ascertain the immunomodulatory efficacy of Hedychium spicatum rhizome powder (HSRP) in chronic toxicity produced by indoxacarb in white leghorn (WLH) cockerels by determining the levels of serum immunoglobin (IgY), Interferon-γ (INF-γ), Interleukin -2 (IL-2), and lymphocyte stimulation test (LST), Macrophage function test (MFT) and delayed type hypersensitivity (DTH). 49 white leghorn (WLH), 6-8 weeks old chicks were divided into 07 groups consisting of 7 birds in each gr. Group I was maintained at grower ration whereas groups II to VII were given medicated ration upto 16 weeks. The immunomodulant activity of HSRP was determined using both Cell mediated immune response (CMI) i.e., LST, MFT and DTH and Humoral mediated immune response (HMI) i.e., by evaluating antibody titre against bovine serum albumin (BSA) used as antigen. Quantification of chicken IL-2 and INF-γ were carried out by ELISA method as per the manufacture instructions using standard curve method. The result of present finding indicates that the indoxacarb producing the immunosuppression in long term intoxication in WLH cockerels and simultaneous feeding of rhizome powder of H spicatum (HSRP) @ 2000ppm and 4000ppm produced immunomodulatory effects in dose dependent manner by elevation of IgY, INF-γ and IL-2 titres. The decreases in these parameters by indoxacarb reveal the immunosuppression and simultaneous treatment with HSRP improves these values indicating the immunomodulatory properties of H spicatum and can be use Ayurvedic medicines alone.

Key words: Hedychium Spicatum; Immunomodulation; Interleukin-2; Interferon-Y; Indoxacarb; White Leghorn Cockerel

References

1. Atal CK., et al. “Immunomodulatory agents of plant origin 1: preliminary screening”. Journal of Ethnopharmacology 18 (1986): 131-141.
2. Wagner H. “Immunoprophylaxe und-therapie durch pflanzenpraeparate”. Zeitschrift fur Allgemeinmedizin 24 (1983): 1282-1289.
3. Wagner H. “Phytoproparate zur immunoprophylaxae and immunotherapie”. Biologische Medizin 1 (1984): 3.
4. Wagner H. “In: Krogsgaard Larsen P, Brogze S, Christensen H (Eds.) Alfred Benzon Sympsoium 20. Koford Munksgaard, Copenhagen (1984).
5. Kumari I., et al. “Hedychium spicatum buch-ham (Kuchri), A treasure house of essential oils”. International Journal of Current Pharmaceutical Research 4 (2021): 25-31.
6. Pandeya, K.B., et al. “A critical review on traditional herbal drugs: An emerging alternative drug for diabetes”. International Journal of Organic Chemistry 3 (2013): 1-22.
7. Thapliyal S., et al. “Pharmacognostic Screening of Hedychium spicatum Rhizomes”. International Journal of Chemistry and Pharmaceutical Sciences 4 (2014): 483-488.
8. Kajaria D., et al. “In-vitro evaluation of immunomodulatory effect of polyherbal compound - Bharangyadi”. Journal of Drug Delivery and Therapeutics 1 (2013): 36-39.
9. Puri A., et al. “Immunostimulant activity of certain plants used in Indian traditional Medicine”. Journal of Medicinal Plants Research 7 (2013): 3242-3246.
10. Singh KL and Bagh GC. “Phytochemical analysis and determination of total phenolics content in water extracts of three species of Hedychium”. International Journal of Pharm Tech Research CODEN (USA)4 (2013): 1516-1521.
11. Sravani T and Paarakh PM. “Antioxidant property of Hedychium spicatum Buch-Ham. Rhizomes”. Indian Journal of Natural Products and Resources 3 (2012): 354-358.
12. Divya K., et al. “Study of antiasthmatic properties and chemical characterization of indigenous ayurvedic compounds (polyherbal formulations)”. American Journal of Phytomedicine and Clinical Therapeutics 6 (2013): 457-466.
13. Choudhary GK and Singh SP. “Cytotoxic potential of rhizome extracts of Hedychium spicatum in HepG2 cell line using MTT”. The Indian Journal of Animal Sciences 87 (2017): 313-315.
14. Thapliyal S., et al. “Hepatoprotective and Antioxidant Activity of Methanol Extract of Hedychium spicatum against CCl4-Induced Liver Injury in Rats”. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2 (2014): 1428.
15. Lapied B., et al. “Indoxacarb, an oxadiazine insecticide, blocks insect neuronal sodium channels”. British Journal of Pharmacology 2 (2001): 587-595.
16. Haas PJ. “Evaluation of subacute toxicity of carbamate on hematological and serobiochemical changes in rats”. American Journal of Veterinary Research 44 (1983): 879.
17. Frame SR. “Oncogenicity study with DPX-JW062-106 (50% DPX-KN128, 50% DPX-KN127) eighteen-month feeding study in mice. Unpublished report No. HLR 799-96 from DuPont Haskell Laboratory, Newark, Delaware, USA”. Submitted to WHO by E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA (1997).
18. Mertens JJWM. “Subchronic oral toxicity: 90-day study with DPX-JW062-106 (50% DPX-KN128, 50% DPX-KN127) feeding study in dogs. Unpublished report No. HLO 494-95, RV3 from WIL Research Laboratories, Inc., Ashland, Ohio, USA”. Submitted to WHO by E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA (1997).
19. Boyse EA., et al. “Cytotoxic test for demonstration of mouse antibody”. Methods in Medical Research 10 (1964): 39-47.
20. Rai-el-Balhaa G., et al. “Lymphoblastic transformation assay of sheep peripheral blood lymphocytes: a new rapid and easy-to-read technique”. Comparative Immunology, Microbiology and Infectious Diseases 3-4 (1985): 311-318.
21. Chauhan RS. “Lymphocyte stimulation test in- laboratory Manual of Immunology for DBT sponsored training course on “Immunopathology: Modern trends in diagnosis and control” (1998): 29-30.
22. Talwar GP. “A Handbook of Practical Immunology”. Vikas Publishing House, Pvt. Ltd., New Delhi (1983).
23. Phanuphak P., et al. “Tolerance and contact sensitivity to DNFB in mice”. The Journal of Immunology 112 (1974): 115-123.
24. Engvall E and Perlmann P. “Enzyme linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G”. IImmunochemistry 8 (1971): 871-874.
25. Chalghoumi R., et al. “Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: A review”. Biotechnologie, Agronomie, Société et Environnement 13 (2009): 295-308.
26. Kumar A., et al. “Effect of indoxacarb induced toxicity on biochemical parameters in broilers (Gallus domesticua)”. Journal Of Veterinary Pharmacology and Toxicology 12 (1-2) (2014): 21-23.
27. Kaur S., et al. “Indoxacarb interaction alters immunotoxic and genotoxic potential of endotoxin”. Journal of Pesticide Science 3 (2016): 65-70.
28. Frame SR. “Oncogenicity study with DPX-JW062-106 (50% DPX-KN128, 50% DPX-KN127) two-year feeding study in rats”. Unpublished report No. HLR 1174-96, RV1 from DuPont Haskell Laboratory, Newark, Delaware (1997).
29. Mertens JWM. “Chronic toxicity study with DPX-JW062-106 (50% DPX-KN128, 50% DPX- KN127) one year feeding study in dogs”. Unpublished report No. HLO 885-96, RV1 from WIL Research Laboratories, Inc., Ashland, Ohio, USA. Submitted to WHO by E.I. du Pont de Nemours and Company, Wilmington, Delaware, USA (1997).
30. Bloom JC and Brandt JT. “Toxic responses of the blood,” In Casarett and Doul’s Toxicology (C. D. Klaassen, Ed.), Mcgraw-Hill, New York (2001): 389-417.
31. Setoguchi R., et al. “Homeostatic maintenance of natural Foxp3+ CD25+ CD4+ regulatory T cells by interleukin (IL)-2 and induction of autoimmune disease by IL-2 neutralization”. Journal of Experimental Medicine 5 (2005): 723-735.
32. Taniguchi T., et al. “Structure and expression of a cloned cDNA for human interleukin-2”. Nature 302 (1983): 305-310.
33. Boyman O and Sprent J. “The role of interleukin-2 during homeostasis and activation of the immune system”. Nature Reviews Immunology 12 (2012): 180-190.
34. Farrar MA and Schreiber RD. “The molecular cell biology of interferon- and its receptor”. Annual Review of Immunology 11 (1993): 571-611. 
35. Oberholzer A., et al. “Cytokine signaling-regulation of the immune response in normal and critically ill states”. Critical Care Medicine 4 (2000): N3-N12.
36. Singh BP., et al. “Immunotoxicity of carbaryl in chicken”. Indian Journal of Experimental Biology 45 (2007): 890-895.
37. Uttara J and Mishra SH. “Preliminary evaluation of immunomodulatory and antistress activity of methanol extract of Hedychium spicatum”. Pharmacologyonline 1 (2009): 1057-1071.
38. Luster MI., et al. “Approches and concepts in immunotoxicology, in: Experimental immunotoxicology”. Edited by R. J. Smialowicz and M. P. Holsapple, (CRC Press, New York) (1996): 13.
39. Danneberg AM. “Delayed type hypersensitivity and cell mediated immunity in the pathogenesis of tuberculosis”. Immunology Today 12 (1991): 228.
40. Malik G., et al. “Biochemical and immunological alteration induced by chlorpyriphos intoxication in broiler chicks”. The Indian Journal of Animal Sciences 6 (2005): 668-671.

Citation

Citation: Govind Kumar Choudhary., et al. “Immunomodulatory Efficacy of Hedychium Spicatum Rhizome Powder in Long Term Indoxacarb Intoxicated White Leghorn (WLH) Cockerels". Acta Scientific Veterinary Sciences 4.9 (2022): 129-142.

Copyright

Copyright: © 2022 Rajeev Ranjan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.