Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 8

Genetic Diversity of Vancomycin Resistance Enterococcus Spp. Isolated from Animal, Human and Environmental Origin

Chaitanya Gottapu1*, Ch. Bindu Kiranmayi2, T. Srinivasa Rao3, K. Aswani Kumar4, N. Subashini5, B. Swathi vimala6, Srinivas K7, Suresh Yasarla8, Chaitanya Venkata6, B. Bhavana 6

1Doctoral student, College of Veterinary Sciences, University of Florida 2Principal, Veterinary Polytechnic college, V.R. Gudem, Sri Venkateswara Veterinary University, Andhra Pradesh, India 3Professor and Head, Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India 4Professor and Head, Department of Veterinary Biochemistry, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India 5Assistant professor, Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India 6M.V.Sc. Scholar, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India 7Ph. D scholar, ICAR – Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India 8Assistant professor, College of Veterinary Science, Tirupati, Andhra Pradesh, India

*Corresponding Author:Chaitanya Gottapu, Doctoral Student, College of Veterinary Sciences, University of Florida, Florida.

Received: July 18, 2022; Published: July 25, 2022

Abstract

Enterococci are the common opportunistic pathogens having a worldwide food safety concern. The present study was undertaken to characterize the vancomycin resistant Enterococcus species of animal, human and environmental origin by using ERIC-PCR and REP-PCR assays. Out of 608 Enterococcus spp. isolates recovered by phenotypic and genotypic methods, 125 Enterococcus isolates were identified as Vancomycin resistance Enterococcus genotypically. The vancomycin resistant genes vanB, vanC1 and vanC2 were detected in 14 (11.20%), 69 (55.20%) and 42 (36.60%) Enterococcus isolates, respectively. A greater degree of heterogeneity was observed among 124 VRE isolates (one E. gallinarum isolate did not yield any bands for both ERIC-PCR and REP- PCR) of four species of Enterococcus from different sources as revealed by presence of 122 genotypes and 123 genotypes by ERIC and REP-PCR analysis, respectively. Nineteen different E. faecalis, 15 E. faecium, 57 E. gallinarum and 31 E. casseliflavus subtypes were differentiated by ERIC-PCR, whereas 21 different E. faecalis, 15 E. faecium, 56 E. gallinarum and 31 E. casseliflavus subtypes by REP- PCR. Genotyping of VRE species by ERIC- PCR and REP- PCR were found to be highly significant since discriminatory power > 0.9 are considered highly significant (0.9997 for ERIC-PCR and 0.9999 for REP-PCR). Cluster analysis also revealed a great degree of homogeneity among some VRE isolates recovered from different sources and implied at the chance of cross-contamination of foods of animal origin.

Keywords: Enterococci; VRE; ERIC-PCR; REP-PCR; Discriminatory Power; Cluster Analysis

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Citation

Citation: Chaitanya Gottapu., et al. “Genetic Diversity of Vancomycin Resistance Enterococcus Spp. Isolated from Animal, Human and Environmental Origin". Acta Scientific Veterinary Sciences 4.8 (2022): 130-146.

Copyright

Copyright: © 2022 Chaitanya Gottapu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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