Genetic Diversity of Vancomycin Resistance Enterococcus Spp. Isolated from
Animal, Human and Environmental Origin
Chaitanya Gottapu1*, Ch. Bindu Kiranmayi2, T. Srinivasa Rao3, K.
Aswani Kumar4, N. Subashini5, B. Swathi vimala6, Srinivas K7, Suresh Yasarla8, Chaitanya Venkata6, B. Bhavana 6
1Doctoral student, College of Veterinary Sciences, University of Florida
2Principal, Veterinary Polytechnic college, V.R. Gudem, Sri Venkateswara Veterinary
University, Andhra Pradesh, India
3Professor and Head, Department of Veterinary Public Health and Epidemiology, NTR
College of Veterinary Science, Gannavaram, Andhra Pradesh, India
4Professor and Head, Department of Veterinary Biochemistry, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
5Assistant professor, Department of Veterinary Public Health and Epidemiology, NTR
College of Veterinary Science, Gannavaram, Andhra Pradesh, India
6M.V.Sc. Scholar, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
7Ph. D scholar, ICAR – Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India
8Assistant professor, College of Veterinary Science, Tirupati, Andhra Pradesh, India
*Corresponding Author:Chaitanya Gottapu, Doctoral Student, College of
Veterinary Sciences, University of Florida, Florida.
July 18, 2022; Published: July 25, 2022
Enterococci are the common opportunistic pathogens having a worldwide food safety concern. The present study was undertaken
to characterize the vancomycin resistant Enterococcus species of animal, human and environmental origin by using ERIC-PCR and
REP-PCR assays. Out of 608 Enterococcus spp. isolates recovered by phenotypic and genotypic methods, 125 Enterococcus isolates
were identified as Vancomycin resistance Enterococcus genotypically. The vancomycin resistant genes vanB, vanC1 and vanC2 were
detected in 14 (11.20%), 69 (55.20%) and 42 (36.60%) Enterococcus isolates, respectively. A greater degree of heterogeneity was
observed among 124 VRE isolates (one E. gallinarum isolate did not yield any bands for both ERIC-PCR and REP- PCR) of four species
of Enterococcus from different sources as revealed by presence of 122 genotypes and 123 genotypes by ERIC and REP-PCR analysis,
respectively. Nineteen different E. faecalis, 15 E. faecium, 57 E. gallinarum and 31 E. casseliflavus subtypes were differentiated by
ERIC-PCR, whereas 21 different E. faecalis, 15 E. faecium, 56 E. gallinarum and 31 E. casseliflavus subtypes by REP- PCR. Genotyping
of VRE species by ERIC- PCR and REP- PCR were found to be highly significant since discriminatory power > 0.9 are considered highly
significant (0.9997 for ERIC-PCR and 0.9999 for REP-PCR). Cluster analysis also revealed a great degree of homogeneity among some
VRE isolates recovered from different sources and implied at the chance of cross-contamination of foods of animal origin.
Keywords: Enterococci; VRE; ERIC-PCR; REP-PCR; Discriminatory Power; Cluster Analysis
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