Ficus Racemose Extract and Vitamin C Ameliorate Paraquat-Induced Oxidative Stress in Wistar Rats
Supriya Yadav1*, Shivendra K Dixit1, Ujjwal K DE1, Suhas KS2 and Pankaj K Patel2
1Department of Veterinary Medicine, Indian Veterinary Research Institute, India
2Department of Veterinary Pharmacology and Toxicology, Indian Veterinary Research Institute, India
*Corresponding Author: Supriya Yadav, Department of Veterinary Medicine, Indian Veterinary Research Institute, India.
June 10, 2022; Published: June 27, 2022
Ficus racemosa is an important medicinal plant found ubiquitously. The immunomodulatory potential of this plant has not been delved much. In the present study, we evaluated the in vivo antioxidant potential of Ficus racemosa in paraquat-induced oxidative stress in Wistar rats. The ethanolic extract of the leaves of F. racemosa was used for the study. For the in vivo study, 5 groups of 6 rats per group, including paraquat-induced oxidative stress were evaluated for responses in a 30-day experimental trial using doses of 250 mg/kg b wt. for F. racemosa extract. Oxidative stress induced by Paraquat caused an increase in ROS, caused severe liver damage, increased lipid peroxidation but not many hematological alterations. Group using F. racemosa extract and combination of Ficus extract and vitamin C showed significantly proficient results by decreasing the LPO production and helped in recovering the liver alterations.
However, further research is needed to identify the different molecular mechanisms involved in mediating the antioxidant response.
Keywords: Ficus Extract; Antioxidant; Lipid Peroxidation; Superoxide Dismutase
- Rahal A., et al. “Oxidative stress, prooxidants, and antioxidants: the interplay”. BioMed Research International 2014 (2014).
- Embuscado ME. “Spices and herbs: Natural sources of antioxidants-a mini review”. Journal of Functional Foods 18 (2015): 811-819.
- Mohiuddin AK and Lia SA. “Medicinal and Biological Investigations of Ficus Racemosa”. Agricultural Revolution 2 (2020): 29-81.
- Sumi SA., et al. “Investigation of the key pharmacological activities of Ficus Racemosa and analysis of its major bioactive polyphenols by HPLC-DAD”. Evidence-Based Complementary and Alternative Medicine 2016 (2016).
- Carr AC. “Vitamin C in pneumonia and sepsis”. Vitamin. C (2020).
- EL-Gendy KS., et al. “The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid”. Food and Chemical Toxicology 1 (2010): 215-221.
- Xu P., et al. “Protective Effect of Vitamin C on Triptolide-induced Acute Hepatotoxicity in Mice through mitigation of oxidative stress”. An Acad Bras Cienc 2 (2019).
- Ahkang S., et al. “In vivo dual effects of vitamin C on paraquat-induced lung damage: dependence on released metals from the damaged tissue”. Free Radical Research 1 (1998): 93-107.
- Lascano R., et al. “Paraquat: an oxidative stress inducer”. Herbicides - Properties, Synthesis and Control of Weeds (2012): 135-148.
- Cochemé HM and Murphy MP. “Complex I is the major site of mitochondrial superoxide production by paraquat”. Journal of Biological Chemistry 4 (2008): 1786-1798.
- Tsukamoto M., et al. “Paraquat-induced membrane dysfunction in pulmonary microvascular endothelial cells”. Pharmacology and Toxicology 3 (2000): 102-109.
- Hosamani R., et al. “Standardized Bacopa monnieri extract ameliorates acute paraquat-induced oxidative stress, and neurotoxicity in prepubertal mice brain”. Nutritional Neuroscience 10 (2016): 434-446.
- Bonilla E., et al. “Paraquat-induced oxidative stress in Drosophila melanogaster: effects of melatonin, glutathione, serotonin, minocycline, lipoic acid and ascorbic acid”. Neurochemical Research 12 (2006): 1425-1432.
- Ahmad I., et al. “Maneb and paraquat-induced modulation of toxicant responsive genes in the rat liver: comparison with polymorphonuclear leukocytes”. Chemico-Biological Interactions 3 (2010): 566-579.
- Kim DO., et al. “Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals”. Journal of Agricultural and Food Chemistry 13 (2002): 3713-3717.
- Van Kampen EJ and Zijlstra WG. “Cyanmethemoglobin method”. Clinica Chimica Acta 6 (1961): 538.
- Placer ZA., et al. “Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems”. Analytical Biochemistry 2 (2003): 359-364.
- Prins HK and Loos JA. “Biochemical Methods in Red Cell Genetics, Academic Press” (1969): 127-129.
- Bergmayer HU. “UV method of catalase assay”. Methods of Enzymatic Analysis 3 (1983): 273.
- Madesh M and Balasubramanian KA. “Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide”. Indian Journal of Biochemistry and Biophysics 3 (1998): 184-188.
- Badgujar PC., et al. “Fipronil induced oxidative stress in kidney and brain of mice: protective effect of vitamin E and vitamin C”. Pesticide Biochemistry and Physiology 118 (2015): 10-18.
- Ismail SM. “Protective effects of vitamin C against biochemical toxicity induced by malathion pesticides in male albino rat”. Journal of Evolutionary Biology Research 1 (2013): 1-5.
- Veerapur VP., et al. “Antidiabetic effect of Ficus Racemosa stem bark in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats: a mechanistic study”. Food Chemistry 132.1 (2012): 186-193.
- Khan N and Sultana S. “Chemomodulatory effect of Ficus Racemosa extract against chemically induced renal carcinogenesis and oxidative damage response in Wistar rats”. Life Sciences 11 (2005): 1194-1210.
- Sophia D and Manoharan S. “Hypolipidemic activities of Ficus Racemosa bark in alloxan induced diabetic rats”. African Journal of Traditional, Complementary and Alternative Medicines 4.3 (2007): 279-288.
- Velayutham R., et al. “Protective effect of tannins from Ficus Racemosa in hypercholesterolemia and diabetes induced vascular tissue damage in rats”. Asian Pacific Journal of Tropical Medicine 5 (2012): 367-373.
- Hong SY., et al. “Effect of vitamin C on plasma total antioxidant status in patients with paraquat intoxication”. Toxicology Letters 1 (2002): 51-59.
- Perla V., et al. “Paraquat toxicity in a mouse embryonic stem cell model”. Toxicology in Vitro 2 (2008): 515-524.
- Jahan IA., et al. “Hypoglycaemic and antioxidant activities of Ficus Racemosa Fruits”. Natural Product Research 23.4 (2009): 399-408.
- Manian R., et al. “The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis and Ficus Racemosa L”. Food Chemistry 107.3 (2008): 1000-1007.
- Khan A., et al. “In vitro Antioxidant and Cytotoxicity Analysis of Leaves of Ficus racemose”. Free Radicals, Antioxidants 1 (2017).
- Amirshahrokhi K and Bohlooli S. “Effect of methylsulfonylmethane on paraquat-induced acute lung and liver injury in mice”. Inflammation5 (2013): 1111-1121.
- Awadalla EA. “Efficacy of vitamin C against liver and kidney damage induced by paraquat toxicity”. Experimental and Toxicologic Pathology 5 (2012): 431-434.
- Badroo IA., et al. “Toxicological impacts of herbicide paraquat dichloride on histological profile (gills, liver, and kidney) of freshwater fish Channa punctatus (Bloch)”. Environmental Science and Pollution Research (2002): 1-14.
- Suntres ZE. “Role of antioxidants in paraquat toxicity”. Toxicology 1 (2002): 65-77.
- Rodrigues da Silva M., et al. “Beneficial effects of ascorbic acid to treat lung fibrosis induced by paraquat”. PLoS One11 (2018): e0205535.
- Ikeda K., et al. “Change in the concentration of vitamins C and E in rat tissues by paraquat administration”. Bioscience, Biotechnology, and Biochemistry 5 (2003): 1130-1131.
- Mandal SC., et al. “Hepatoprotective activity of Ficus Racemosa leaf extract on liver damage caused by carbon tetrachloride in rats”. Phytotherapy Research 5 (1999): 430-432.