Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Mini Review Article Volume 4 Issue 7

Microsatellite Markers in Fisheries

Gowrimanohari Rakkannan*

College of Fisheries, CAU(I), Imphal, India

*Corresponding Author: Gowrimanohari Rakkannan, College of Fisheries, CAU(I), Imphal, India.

Received: June 16, 2022; Published: June 24, 2022


Simple Sequence Repeats (SSR) or microsatellites are the genetic markers which are highly abundant and evenly distributed in eukaryotic genome. They have become the ideal markers for a wide range of population genetic, conservation, and evolutionary biology applications. Microsatellites are highly polymorphic because they have many alleles that are highly variable among individuals. Polymorphism is produced by having various numbers of tandem repeat motifs, which results in size variation that may be observed using PCR using pairs of locus-specific flanking primers and electrophoresis of the amplified product.

Keywords:Polymorphism; Locus-Specific Flanking Primers; PCR; Electrophoresis


  1. Abdul-Muneer PM. "Application of microsatellite markers in conservation genetics and fisheries management: recent advances in population structure analysis and conservation strategies”. Genetics Research International2014 (2014).
  2. Bekkevold Dorte., et al. “Male reproductive competition in spawning aggregations of cod (Gadus morhua,)”. Molecular Ecology11.1 (2002): 91-102.
  3. Edwards Albert., et al. “DNA typing and genetic mapping with trimeric and tetrameric tandem repeats”. American Journal of Human Genetics4 (1991): 746.
  4. Estoup A. "Microsatellites and minisatellites for molecular ecology: theoretical and empirical consideration”. Advances in Molecular Ecology(1998).
  5. Goldstein David B., et al. “An evaluation of genetic distances for use with microsatellite loci”. Genetics1 (1995): 463-471.
  6. Hallerman EM. “Population genetics: principles and applications for fisheries scientists”. American Fisheries Society, Bethesda, Maryland (2003): 458.
  7. Jeffreys, Alec J., et al. “Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA”. Nature6161 (1988): 278-281.
  8. Litt, Michael and Jeffrey A Luty. "A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene”. American Journal of Human Genetics3 (1989): 397.
  9. Liu ZJ and JF Cordes. "DNA marker technologies and their applications in aquaculture genetics”. Aquaculture1-4 (2004): 1-37.
  10. Liu Zhanjiang., et al. “Microsatellite-containing genes from the channel catfish brain: evidence of trinucleotide repeat expansion in the coding region of nucleotide excision repair gene RAD23B”. Biochemical and Biophysical Research Communications2 (2001): 317-324.
  11. Luo Wei., et al. “Rapid development of microsatellite markers for the endangered fish Schizothorax biddulphi (Günther) using next generation sequencing and cross-species amplification”. International Journal of Molecular Sciences11 (2012): 14946-14955.
  12. May Bernie. "Allozyme variation”. Population Genetics: Principles and Applications for Fisheries Scientists. American Fisheries Society, Bethesda, Maryland(2003): 23-36.
  13. O'connell Michael and Jonathan M Wright. "Microsatellite DNA in fishes”. Reviews in Fish Biology and Fisheries3 (1997): 331-363.
  14. O'Connell Michael., et al. “Differentiation of rainbow trout (Oncorhynchus mykiss) populations in Lake Ontario and the evaluation of the stepwise mutation and infinite allele mutation models using microsatellite variability”. Canadian Journal of Fisheries and Aquatic Sciences6 (1997): 1391-1399.
  15. Okumuş İbrahim and Yılmaz Çiftci. "Fish population genetics and molecular markers: II-molecular markers and their applications in fisheries and aquaculture”. Turkish Journal of Fisheries and Aquatic Sciences1 (2003).
  16. Queller David C., et al. “Microsatellites and kinship”. Trends in Ecology and Evolution8 (1993): 285-288.
  17. Reilly Anne., et al. “Genetic differentiation between Tasmanian cultured Atlantic salmon (Salmo salar) and their ancestral Canadian population: comparison of microsatellite DNA and allozyme and mitochondrial DNA variation”. Aquaculture173.1-4 (1999): 459-469.
  18. Saiki Randall K., et al. “Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase”. Science4839 (1988): 487-491.
  19. Sundaray Jitendra Kumar., et al. “Simple sequence repeats (SSRs) markers in fish genomic research and their acceleration via next-generation sequencing and computational approaches”. Aquaculture International4 (2016): 1089-1102.
  20. Tautz Diethard and Manfred Renz. "Simple sequences are ubiquitous repetitive components of eukaryotic genomes”. Nucleic Acids Research10 (1984): 4127-4138.
  21. Tautz Diethard. "Hypervariability of simple sequences as a general source for polymorphic DNA markers”. Nucleic Acids Research16 (1989): 6463-6471.
  22. Tessier N., et al. “Population structure and impact of supportive breeding inferred from mitochondrial and microsatellite DNA analyses in land‐locked Atlantic salmon Salmo salar L”. Molecular Ecology8 (1997): 735-750.
  23. Weber James L. "Informativeness of human (dC-dA) n·(dG-dT) n polymorphisms”. Genomics4 (1990): 524-530.
  24. Wright JM. "DNA fingerprinting of fishes”. Biochemistry and Molecular Biology of Fishes2 (1993): 57-91.
  25. Zardoya, Rafael., et al. “Evolutionary conservation of microsatellite flanking regions and their use in resolving the phylogeny of cichlid fishes (Pisces: Perciformes)”. Proceedings of the Royal Society of London. Series B: Biological Sciences1376 (1996): 1589-1598.


Citation: Gowrimanohari Rakkannan. “Microsatellite Markers in Fisheries". Acta Scientific Veterinary Sciences 4.7 (2022): 103-106.


Copyright: © 2022 Gowrimanohari Rakkannan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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