Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 7

Oligo-Probe Conjugated Gold Nanoparticles for Rapid Detection of Unamplified Bluetongue Viral RNA

Upendra P Lambe, Basanti Brar and Prasad Minakshi*

Department of Animal Biotechnology, Lala Lajpatrai University of Veterinary and Animal Sciences, Hisar, India

*Corresponding Author: Prasad Minakshi, Department of Animal Biotechnology, Lala Lajpatrai University of Veterinary and Animal Sciences, Hisar, India.

Received: May 12, 2022; Published: June 15, 2022

Abstract

Bluetongue (BT) is an insect born, non-fatal disease of small ruminants, particularly sheep; characterized by inflammation of buccal mucosa and coronary bands, nasal discharge, facial edema and cyanosis of the tongue, hence the name Bluetongue. The etiological agent being BT virus belonging to the Reoviridae family is a ds-RNA virus with a segmented genome. Though the disease is non-fatal, causes significant losses to wool and meat industry due to decreased wool production and weight losses in animals. Gold nanoparticles (GNPs) is regarded as one of the most stable nanoparticles and also because of their surface charge, they are able to be activated with majority of bio-molecules. Hence in this study we have developed a novel GNPs-based nucleic probe for detection of BT virus with sensitivity of 14.4pg/ul. The GNPs based robust technique can be implemented for diagnosis of other Arboviruses as a potential point of care assay. There is no earlier report on designing of nucleic acid nanoparticles-based assay for detection of Bluetongue virus in India and abroad to the best of our knowledge.

Keywords: Nanoparticles; Unamplified; Bluetongue; Viral RNA

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Citation

Citation: Upendra P Lambe., et al. “Oligo-Probe Conjugated Gold Nanoparticles for Rapid Detection of Unamplified Bluetongue Viral RNA". Acta Scientific Veterinary Sciences 4.7 (2022): 27-34.

Copyright

Copyright: © 2022 Prasad Minakshi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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