Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 6

Histoenzymic Localization of Phosphatases, Oxidoreductases and Nonspecific Esterase in the Uterus of Canines Collected After Ovariohysterectomy

Kaveesh Chahal1, Devendra Pathak1*, Opinder Singh1, Ashwani Kumar2 and Kuldip Gupta3

1Department of Veterinary Anatomy, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of Veterinary Surgery and Radiology, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
3Department of Veterinary Pathology, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

*Corresponding Author: Devendra Pathak, Department of Veterinary Anatomy, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.

Received: May 13, 2022; Published: May 27, 2022

Abstract

The present investigation was aimed at elucidating the localization patterns of the phosphatases, oxidoreductases, and non-specific esterase in the uterus of canines collected after ovariohysterectomy and correlating these enzymes with the stage of reproduction and disease conditions. Cryostat sections of 6-7 μ thickness at -20°C were obtained on glass slides with cryostat microtome and incubated with substrates of different enzymes. A strongly positive reaction of phosphatases and oxidoreductases was seen in the epithelium lining the endometrium and endometrial glands, and blood vessels in the prepubertal, cyclical, and gravid uterus. There was spatial localization of enzymes some of the enzymes (AKPase) were localized in the basal region of epithelium and glands which might be indicative of cellular transport towards the basal compartment while most of the enzymes were localized in the apical region of the cells or in dispersed in the cytoplasm of the cells. The reaction of these enzymes in the diseased uterus was strong to intense in papillary endometrial hyperplasia, cystic papillary endometrial hyperplasia, and cystic endometrial hyperplasia and that could be due to degenerative changes occurring due to infections. It was concluded that the reactivity was higher in the epithelium, glands of pubertal and pregnant animals as compared to the prepubertal group, while the reactivity of most of the enzymes was stronger in diseased animals.

Keywords: Canines; Esterase; Histoenzymic; Oxidoreductases; Phosphatases; Uterus

References

  1. Emadi SM and M Salehnia. "Localization and activity of mouse endometrial alkaline phosphatase after hyperstimulation and progesterone injection at the implantation time”. Iranian Biomedical Journal 8 (2004): 121-126.
  2. Roy SC., et al. “Changes in uterine protein secretion during luteal and follicular phases and detection of phosphatases during the luteal phase of the estrous cycle in buffaloes (Bubalus bubalis)”. Theriogenology7 (2006): 1292-1301.
  3. Pathak Devendra and Neelam Bansal. "Histoenzymic studies on the ovary of Indian buffalo during different reproductive stages”. Buffalo Bulletin1 (2019): 19-33.
  4. Barka Tibor and Paul J Anderson. “Histochemistry: theory, practice, and bibliography”. Hoeber Medical Division, Harper and Row (1963).
  5. Pearse Anthony G. "Histochemistry, theoretical and applied”. Vol. II. Churchill Livingstone, London. (1953).
  6. Mishra OP., et al. “Study on biochemical constituents of caprine ovarian follicular fluid after superovulation”. Asian-Australasian Journal of Animal Sciences12 (2003): 1711-1715.
  7. Sangha GK and SS Guraya. "Histochemical changes in acid and alkaline phosphatase activities in the growing follicles and corpora lutea of the rat ovary”. Acta Morphologica Neerlando-Scandinavica1 (1988): 43-49.
  8. Jaiswal RS and VM Mehta. "Alkaline-phosphatase and peroxidase-activities in uterus and vagina of Marwari goats during estrous-cycle”. Indian Journal of Animal Sciences5 (1992): 429-431.
  9. Larson LL., et al. “Cyclic changes in bovine endometrium”. Journal of Animal Science 3. 1111. North Dunlap Ave, Savoy, IL 61874: American Society of Animal Science (1965).
  10. Kumar Vijay., et al. “Acid and Alkaline Phosphatase Activity in Uterus of Goat in Different Stage of Pregnancy”. International Journal of Livestock Research7 (2017): 92-97.
  11. Uppal V and KS Roy. "Histoenzymic distributions of phosphatases, esterases and oxidoreductases in the uterus of cyclic and pregnant buffaloes (Bubalus bubalis)”. Buffalo Journal2 (2000): 193-205.
  12. Zamiri MJ and Alan W Blackshaw. "Enzyme histochemistry of the sheep uterus during the oestrous cycle”. Australian Journal of Biological Sciences5 (1979): 409-414.
  13. Motta P and ES Hafez. “Biology of the Ovary”. Springer Science and Business Media 12(2012).
  14. Chayen, J., et al. "A guide for practical histochemistry”. Oliver and Boyol, Edinburgh, England (1969): 207.
  15. Roy KS and RP Saigal. "Histoenzymic study on the uterus and early placentome of the sheep”. Indian Journal of Animal Sciences 7 (1987): 695-699.
  16. Krajničáková M., et al. “Activity of alkaline and acidic phosphatase and non-specific esterase in the endometrium and oviduct of postpartum does”. Small Ruminant Research1-2 (2004): 47-51.
  17.  

Citation

Citation: Devendra Pathak., et al. “Histoenzymic Localization of Phosphatases, Oxidoreductases and Nonspecific Esterase in the Uterus of Canines Collected After Ovariohysterectomy". Acta Scientific Veterinary Sciences 4.6 (2022): 75-83.

Copyright

Copyright: © 2022 Devendra Pathak., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is September 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US