Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 6

Histoenzymic Localization of Phosphatases, Oxidoreductases and Nonspecific Esterase in the Uterus of Canines Collected After Ovariohysterectomy

Kaveesh Chahal1, Devendra Pathak1*, Opinder Singh1, Ashwani Kumar2 and Kuldip Gupta3

1Department of Veterinary Anatomy, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of Veterinary Surgery and Radiology, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
3Department of Veterinary Pathology, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

*Corresponding Author: Devendra Pathak, Department of Veterinary Anatomy, College of Veterinary Science (Ludhiana), Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.

Received: May 13, 2022; Published: May 27, 2022

Abstract

The present investigation was aimed at elucidating the localization patterns of the phosphatases, oxidoreductases, and non-specific esterase in the uterus of canines collected after ovariohysterectomy and correlating these enzymes with the stage of reproduction and disease conditions. Cryostat sections of 6-7 μ thickness at -20°C were obtained on glass slides with cryostat microtome and incubated with substrates of different enzymes. A strongly positive reaction of phosphatases and oxidoreductases was seen in the epithelium lining the endometrium and endometrial glands, and blood vessels in the prepubertal, cyclical, and gravid uterus. There was spatial localization of enzymes some of the enzymes (AKPase) were localized in the basal region of epithelium and glands which might be indicative of cellular transport towards the basal compartment while most of the enzymes were localized in the apical region of the cells or in dispersed in the cytoplasm of the cells. The reaction of these enzymes in the diseased uterus was strong to intense in papillary endometrial hyperplasia, cystic papillary endometrial hyperplasia, and cystic endometrial hyperplasia and that could be due to degenerative changes occurring due to infections. It was concluded that the reactivity was higher in the epithelium, glands of pubertal and pregnant animals as compared to the prepubertal group, while the reactivity of most of the enzymes was stronger in diseased animals.

Keywords: Canines; Esterase; Histoenzymic; Oxidoreductases; Phosphatases; Uterus

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Citation

Citation: Devendra Pathak., et al. “Histoenzymic Localization of Phosphatases, Oxidoreductases and Nonspecific Esterase in the Uterus of Canines Collected After Ovariohysterectomy". Acta Scientific Veterinary Sciences 4.6 (2022): 75-83.

Copyright

Copyright: © 2022 Devendra Pathak., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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