Shanti Choudhary*, Binita Nautiyal and Sanjeev Kumar

Department of Animal Science, College of Animal Biotechnology, GADVASU, Ludhiana, Punjab, M.J.P. Rohilkhand University, Bareilly, (U.P.), India

*Corresponding Author: Shanti Choudhary, Department of Animal Science, College of Animal Biotechnology, GADVASU, Ludhiana, Punjab, M.J.P. Rohilkhand University, Bareilly, (U.P.), India.

Received: May 02, 2022; Published: May 19, 2022

Abstract

The interferon-gamma (INFG) is a significant modulator of macrophage function, one of the candidate genes implicated in the immune response to various avian diseases. The objective of this study was to explore the polymorphism of INFG gene promoter of Aseel, the Indian native chicken using polymerase chain reaction -Restriction Fragment Length Polymorphism (PCR-RFLP). Based on immunological traits, 48 Aseel birds (24 males with high and low titer and 24 females with high and low titer values) were selected from 301 birds. DNA was isolated from blood, and PCR amplification of the INFG promoter region was conducted using two sets of primer, set I for the full-length promoter and set II for a partial-length promoter. Primer set I and set II to produce a product length of 670 bp and 495 bp, respectively. Agarose gel electrophoresis of PCR amplified product and DNA sequencing showed amplification of the INFG gene promoter region. The RFLP analysis of full-length promoter with enzymes, EcoR1 and Taq 1 and partial length promoter with enzymes Alu 1, Hinf1, Dde 1, and Taq 1 was monomorphic. However, the full-length promoter with enzyme Tsp5091 was polymorphic and produced 168, 123, 104, 99, 88, 64, and 54 bp size fragments. Allele A (168 bp, 123 bp, 99 bp, 88 bp, 64 bp, and 54 bp fragments) and allele B (123 bp, 104 bp, 99 bp, 88 bp, 64 bp, and 54 bp fragments) showed all possible genotypes A.A., AB, and B.B. with genotypic frequencies of 0.17, 0.30 and 0.53, respectively. Heterozygotes demonstrated more immunocompetence traits (H.A. titer to SRBC, serum lysozyme activity, and IgG level). The INFG gene promoter was mainly monomorphic, and polymorphism was associated with high immunocompetence in Aseel. We conclude that DNA analysis of the IFNG promoter gene may serve as a genetic marker for higher immune responsiveness.

Keywords: Aseel; INFG; polymorphism; PCR-RFLP; Immunocompetence

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Citation

Citation: Shanti Choudhary., et al. “Polymorphism of Interferon-Gamma (INFG) Gene Promoter in High and Low Immunocompetent Aseel Chicken using PCR-RFLP". Acta Scientific Veterinary Sciences 4.6 (2022): 39-44.

Copyright

Copyright: © 2022 Shanti Choudhary et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.