Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 5

Identification of Associations Between Ucp2 and Ucp3 Gene Polymorphisms and Meat Quantity Traits in Three Indigenous Cattle Breeds of Pakistan

Muhammad Mehran Mouzam* Amna Awan and Watiba Danish

University of Agriculture Faisalabad

*Corresponding Author: Muhammad Mehran Mouzam, University of Agriculture Faisalabad..

Received: March 28, 2022; Published: April 08, 2022


In identifying mutations occurring in distinct cow breeds, genetic elements must be taken into consideration. More recently, these hereditary features have gained attention throughout the world. As in many underdeveloped nations, to bridge the deficit in molecular genetics, multiple solutions are required. The inner membrane anion carrier superfamily contains the uncoupling proteins (UCPs), vital to energy regulation. Research on heredity has shown that variations in the Ucp2 and Ucp3 genes are connected to obesity and metabolic syndrome. This research aimed to investigate if any mutation in the Ucp2 and Ucp3 genes are related to many characteristics in Pakistan's three indigenous cattle breeds. The results of this study revealed 07 variations in the Exon 4 region of the Ucp2 gene and 03 variants in the Exon 3 region of the Ucp3 gene using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) followed by Sequencing among 215 indigenous cattle breeds. The association study revealed that the g.C35G mutation in the Ucp3 gene is strongly related to meat quantity characteristics such as carcass weight and drip percentage (P0.05), but not with body height or hip-width (P > 0.05). Sequence analysis showed five distinct diplotypes: AA, BC, AC, CC, and CD. Cattle with the novel heterozygous diplotype BC perform better in carcass trait and drip percentage than animals with other genotypes. The study's findings suggest that the Ucp3 gene may be utilized for marker-assisted selection (MAS) and breed mixing in Pakistan cattle breeds to aid in the country's economic growth.

Keywords: DNA; PCR; Gel Electrophoresis; Variations; Ucp2 Gene; Ucp3 Gene


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Citation: Muhammad Mehran Mouzam., et al. “Identification of Associations Between Ucp2 and Ucp3 Gene Polymorphisms and Meat Quantity Traits in Three Indigenous Cattle Breeds of Pakistan".Acta Scientific Veterinary Sciences 4.5 (2022): 38-44.


Copyright: © 2022 Muhammad Mehran Mouzam., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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