Muhammad Mehran Mouzam* Amna Awan and Watiba Danish

University of Agriculture Faisalabad

*Corresponding Author: Muhammad Mehran Mouzam, University of Agriculture Faisalabad..

Received: March 28, 2022; Published: April 08, 2022

Abstract

In identifying mutations occurring in distinct cow breeds, genetic elements must be taken into consideration. More recently, these hereditary features have gained attention throughout the world. As in many underdeveloped nations, to bridge the deficit in molecular genetics, multiple solutions are required. The inner membrane anion carrier superfamily contains the uncoupling proteins (UCPs), vital to energy regulation. Research on heredity has shown that variations in the Ucp2 and Ucp3 genes are connected to obesity and metabolic syndrome. This research aimed to investigate if any mutation in the Ucp2 and Ucp3 genes are related to many characteristics in Pakistan's three indigenous cattle breeds. The results of this study revealed 07 variations in the Exon 4 region of the Ucp2 gene and 03 variants in the Exon 3 region of the Ucp3 gene using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) followed by Sequencing among 215 indigenous cattle breeds. The association study revealed that the g.C35G mutation in the Ucp3 gene is strongly related to meat quantity characteristics such as carcass weight and drip percentage (P0.05), but not with body height or hip-width (P > 0.05). Sequence analysis showed five distinct diplotypes: AA, BC, AC, CC, and CD. Cattle with the novel heterozygous diplotype BC perform better in carcass trait and drip percentage than animals with other genotypes. The study's findings suggest that the Ucp3 gene may be utilized for marker-assisted selection (MAS) and breed mixing in Pakistan cattle breeds to aid in the country's economic growth.

Keywords: DNA; PCR; Gel Electrophoresis; Variations; Ucp2 Gene; Ucp3 Gene

References

1. Li J., et al. “UCP2 gene polymorphisms in obesity and diabetes, and the role of UCP2 in cancer”. FEBS Letters 593 (2019): 2525-2534.
2. Fisler JS and Warden CH ., et al. Nutrition and Metabolism (London) (2006).
3. Azzu V., et al. “The regulation and turnover of mitochondrial uncoupling proteins”. Biochimica et Biophysica Acta 35 (2010): 298-307.
4. Reviews HF-N. “Uncoupling proteins: beyond brown adipose tissue”. Nutrition Reviews 6 (1998): 185-189.
5. Pecqueur C., et al. “Uncoupling protein 2, in vivo distribution, induction upon oxidative stress, and evidence for translational regulation”. Journal of Biological Chemistry 276 (2001): 8705-8712.
6. Zhao RZ., et al. “Mitochondrial electron transport chain, ROS generation and uncoupling (Review)”. International Journal of Molecular Medicine 44 (2019): 3-15.
7. Ricquier D and Bouillaud F. “Mitochondrial uncoupling proteins: From mitochondria to the regulation of energy balance”. The Journal of Physiology 529 (2000): 3-10.
8. Clapham A., et al. “Categories of corporate complicity in human rights abuses” 24 (2001).
9. Affourtit C., et al. “Uncoupling protein-2 contributes significantly to high mitochondrial proton leak in INS-1E insulinoma cells and attenuates glucose-stimulated insulin secretion”. Biochemical Journal 409 (2008): 199-204.
10. Khan M., et al. “Genetic resources and diversity in Pakistani cattle”. Pakistan Veterinary Journal 28 (2008): 95-102.
11. Olfert E., et al. “Guide To the Care and Use of Experimental Animals” 2 (1985).
12. Guha P., et al. “A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples”. Journal of Clinical Laboratory Analysis 32 (2018).
13. Hubbard T., et al. “The Ensembl genome database project”. Nucleic Acids Research 30 (2002): 38-41.
14. Badi MA., et al. “Rapid and optimized protocol for efficient PCR-SSCP genotyping for wide ranges of species”. Biologia (Bratisl) 76 (2021): 2413-2420.
15. Arunachalam K and Sasidharan SP. “Gel Electrophoresis and PCR Amplification”. Bioassays in Experimental and Preclinical Pharmacology New York, NY (2021): 241-258.
16. Purwanto A., et al. “Education Research Quantitative Analysis for Little Respondents (2021).
17. Nkrumah JD., et al. “Different measures of energetic efficiency and their phenotypic relationships with growth, feed intake and ultrasound and carcass merit in hybrid cattle”. Journal of Animal Science 8 (2004): 2451-2459.
18. Brand M., et al. “Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3”. Cell Metabolism 2 (2005): 85-93.
19. Sherman E., et al. “Polymorphisms and haplotypes in the bovine neuropeptide Y, growth hormone receptor, ghrelin, insulin-like growth factor 2, and uncoupling proteins 2 and 3 genes”. Journal of Animal Science 86 (2008): 1-16.
20. Azzu V., et al. “The on-off switches of the mitochondrial uncoupling proteins”. Trends in Biochemical Sciences 35 (2010): 298-307.
21. Donadelli M., et al. “UCP2, a mitochondrial protein regulated at multiple levels”. Cellular and Molecular Life Sciences 71 (2014): 1171-1190.
22. Srivastava N., et al. “A common polymorphism in the promoter of UCP2 is associated with obesity and hyperinsulenemia in northern Indians”. Molecular and Cellular Biochemistry 337 (2010): 293-298.
23. Andersen G., et al. “The frequent UCP2- 866G> A polymorphism protects against insulin resistance and is associated with obesity: a study of obesity and related metabolic traits among”. International Journal of Obesity (London) 37 (2012): 1775-181.
24. Say Y., et al. “Uncoupling protein 2 gene (UCP2) 45-bp I/D polymorphism is associated with adiposity among Malaysian women”. Journal of Biosciences 39 (2014): 867-875.
25. Cieslak J., et al. “Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits”. Meat Science 83 (2009): 551-554.
26. Han X., et al. “Investigation of four porcine candidate genes (H-FABP, MYOD1, UCP3 and MASTR) for meat quality traits in Large White pigs”. Molecular Biology Reports 6 (2012): 6599-6605.
27. Brondani L de A., et al. “Association of the UCP polymorphisms with susceptibility to obesity: case-control study and meta-analysis”. Molecular Biology Reports 41 (2014): 5053-5067.
28. Komar AA. “Silent SNPs: Impact on gene function and phenotype”. Pharmacogenomics 8 (2007): 1075-1080.
29. Liu Y., et al. “Molecular cloning, expression and characterization of bovine UQCC and its association with body measurement traits”. Molecules and Cells 30 (2010): 393-401.
30. He H., et al. “Assessment of haplotype variation in bovine AMPD1 gene for association with growth and carcass traits in Qinchuan beef cattle”. Archives Animal Breeding 54 (2011): 18-26.

Citation

Citation: Muhammad Mehran Mouzam., et al. “Identification of Associations Between Ucp2 and Ucp3 Gene Polymorphisms and Meat Quantity Traits in Three Indigenous Cattle Breeds of Pakistan".Acta Scientific Veterinary Sciences 4.5 (2022): 38-44.

Copyright

Copyright: © 2022 Muhammad Mehran Mouzam., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.