Isolation and Biochemical Characterization of Methicillin Resistant Staphylococcus aureus from Poultry Carcass Rinse in Sokoto Live-Birds Market Slaughter Section
Farida Abubakar
Usmanu Danfodiyo University Sokoto, Nigeria
*Corresponding Author:Farida Abubakar, Usmanu Danfodiyo University Sokoto, Nigeria.
Received:
December 20, 2021; Published: March 28, 2022
Abstract
This study was carried out in Sokoto state Nigeria, to determine the prevalence of MRSA in poultry carcass rinse. A total of 122 samples of poultry carcass rinse were taken using distilled water to carefully rinse poultry carcass and safely transport the carcass rinse to the laboratory for inoculation. Three samples of Tab water used in the slaughter slab was also taken, one before and one after rinsing broiler chicken was taken and another sample after rinsing local chicken was taken. Out of the one hundred and twenty-two samples collected, 37.7% (46/122) were positive on Mannitol salt agar for S. aureus out of which 42.6% (26/61) of broilers were positive for S. aureus and 32.8% (20/61) of local chicken are positive for S. aureus. Following subculture on Oxacillin Resistance Screening Agar Base (ORSAB), 30.3% (37/122) were positive out of which 39.3% (24/61) were broilers and 21.3% (13/61) were local chickens. From the three samples of Tab water used in the slaughter slab, one from broiler chicken and one other from local chicken were all positive on Mannitol salt agar and ORSAB and the sample of water before being used for carcass rinse was positive for S. aureus on Mannitol salt agar and negative on ORSAB.
Keywords: Mannitol Salt Agar; Methicillin Resistance Screening Agar Base; Local Chicken; Broiler Chicken; Slaughter Slab; Staphylococcus aureus
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